literature

SARS_CoV_2 mutation literature information.

Emergence of SARS-CoV-2 Variant B.1.575.2, Containing the E484K Mutation in the Spike Protein, in Pamplona, Spain, May to June 2021.

PMID: 34495709 2021 Journal of clinical microbiology

Result: Between week 20 and week 26 of 2021, we identified 194 cases with lineage B.1.575, which had acquired another S mutation, E484K, classified in the GISAID EpiCoV and Pangolin databases as representing sublineage B.1.575.2.

Result: In contrast, we did not identify any genomes of the B.1.575 or B.1.575.1 lineage carrying the E484K mutation.

Result: In the TaqMan assay, all samples identified by sequencing as B.1.575.2 showed the E484K mutation.

Understanding the molecular interaction of SARS-CoV-2 spike mutants with ACE2 (angiotensin converting enzyme 2).

PMID: 34495817 2021 Journal of biomolecular structure & dynamics

Introduction: For this purpose: i) two single mutants (N501Y and E484K) and a multiple-mutant (K417N + N501Y + E484K) were constructed on the initial crystallographic structure of the spike protein, ii) the obtained mutant structures were submitted to energy minimization, iii) protein-protein dockings were performed on the HADDOCK server, iv) the docked spike/ACE2 conformations with the most negative binding energy (including control) were subjected to 200-nanosecond long molecular dynamics simulations in order to observe the stability of these interactions and, v) the binding free energies of all complexes were calculated using the endpoint MM-PBSA method.

Introduction: Furthermore, vaccines manufactured by Novavax and Johnson & Johnson which have b

Breakthrough Infections of E484K-Harboring SARS-CoV-2 Delta Variant, Lombardy, Italy.

PMID: 34499599 2021 Emerging infectious diseases

Introduction:

Introduction: E484K causes resistance to many class 2 RBD-directed antibodies, including bamlanivimab.

Introduction: E484K has been additionally reported in 1 of 6,011 B.1.617.1 (Kappa variant) sequences.

Possible Link between Higher Transmissibility of Alpha, Kappa and Delta Variants of SARS-CoV-2 and Increased Structural Stability of Its Spike Protein and hACE2 Affinity.

PMID: 34502041 2021 International journal of molecular sciences

Introduction: Further, the B.1.618 (triple mutant), recently detected in the four Indian states (Maharashtra, Delhi, West Bengal and Chhattisgarh), has been characterized by the deletion of Tyr145 and His146 as well as E484K and D614G mutation in the spike protein (accessed on 10 July 2021).

Introduction: In October 2020, B.1.351 (Beta variant) was detected in the South African population, which could infect more younger people and had three primary mutations in the RBD of spike protein, namely, N501Y, K417N and E484K.

Introduction: Similarly, the lineage P.1 (Gamma variant) detected in January 2021 in the Brazilian population had three mutations of concern in spike&nbs

Dynamics prediction of emerging notable spike protein mutations in SARS-CoV-2 implies a need for updated vaccines.

PMID: 34508827 2021 Biochimie

Discussion: However, some variants that have destabilized the protein with increased flexibility (e.g., E484K, K417 N, E484Q, Q675P, and Q675H) might impact the virus's ability to escape the antibodies neutralization by changing the antigenicity drift of the protein 3D structure; this is in agreement with a previous study.

Discussion: Moreover, the results from DynaMut thermodynamic predictions indicated 10 variants had destabilized the spike protein (e.g., E484K, K417 N, V1176F, E484Q, Q675P, and Q675H), two of them, the E484K and the

Probing the Increased Virulence of Severe Acute Respiratory Syndrome Coronavirus 2 B.1.617 (Indian Variant) From Predicted Spike Protein Structure.

PMID: 34513478 2021 Cureus

Introduction: Mutations at this same position (E484K) have been observed in the variants Gamma and Zeta, as well as in the variant Beta.

Introduction: Mutations in the Beta and Gamma variants, including E484K and K417N/T, are of high concern since they partly compromise neutralization generated by previous infection or vaccination or affect viral stability.

Computational decomposition reveals reshaping of the SARS-CoV-2-ACE2 interface among viral variants expressing the N501Y mutation.

PMID: 34516024 2021 Journal of cellular biochemistry

Abstract: These SARS-Cov-2 variants comprise the two Alpha variants (B.1.1.7, United Kingdom and B.1.1.7 with the additional E484K mutation), the Beta variant (B.1.351, South Africa), and the Gamma variant (P.1, Brazil).

Emerging vaccine-breakthrough SARS-CoV-2 variants.

PMID: 34518803 2021 ArXiv

Abstract: Finally, we have identified the co-mutations that have the great likelihood of becoming dominant: [A411S, L452R, T478K], [L452R, T478K, N501Y], [V401L, L452R, T478K], [K417N, L452R, T478K], [L452R, T478K, E484K, N501Y], and [P384L, K417N, E484K, N501Y].

Introduction: By analyzing

Bispecific antibodies targeting distinct regions of the spike protein potently neutralize SARS-CoV-2 variants of concern.

PMID: 34519517 2021 Science translational medicine

Abstract: Furthermore, a bispecific antibody that neutralized the Beta variant protected hamsters against SARS-CoV-2 expressing the E484K mutation.

Safety and immunogenicity of SARS-CoV-2 variant mRNA vaccine boosters in healthy adults: an interim analysis.

PMID: 34526698 2021 Nature medicine

Method: SARS-CoV-2 neutralizing antibodies were quantified using lentivirus particles that incorporate SARS-CoV-2 S protein (Wuhan-Hu-1 isolate mutated to contain D614G) or the B.1.351 variant S protein (L18F-D80A-D215G- L242- A243- L244-K417N-E484K-N501Y-D614G-A701V) on their surface and express firefly luciferase reporter gene for quantitative measurements of infection by relative luminescence units (RLUs) as described.

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