In silico investigations of heparin binding to SARS-CoV-2 variants with a focus at the RBD/ACE2 interface.
PMID: 35194375
2022
Process biochemistry (Barking, London, England)
Abstract: Herein, using the molecular docking and interaction energy analysis, we showed that N501Y, L452R-E484Q, and E484K mutations bind strongly with heparin in the range of - 7.4 to - 8.0 kcal/mol.
Abstract: The triple mutations, K417N-E484K-N501Y, and K417T-E484K-N501Y displayed weaker binding affinity to heparin (-6.6 kcal/mol).
Introduction: The variant, B.1.351 and B.1.1.28 carries 9 and 11 mutations in the spike (S) protein, respectively, including three mutations in the receptor binding domain ( PMID: 35194675
2022
Archives of virology
Introduction: Following the scheme described above, in August 2021, we detected three strains that presented K417N, N501Y, and E484K mutations but did not present delH69/V70, W152C, K417T, L452R, or V1176F mutations.
Introduction: Samples containing E484K and N501Y mutations and lacking delH69/V70 mutation were also subjected to rRT-PCR melting curve analysis of K417N and V1176F (VirSNiP Assays, TIB MOLBIOL, Germany), as this assay was commercially available before the Allplex SARS-CoV-2 Variants II Assay.
Introduction: The first assay, A
Table: E484K
Spread of Gamma (P.1) Sub-Lineages Carrying Spike Mutations Close to the Furin Cleavage Site and Deletions in the N-Terminal Domain Drives Ongoing Transmission of SARS-CoV-2 in Amazonas, Brazil.
Discussion: One of the most divergent Gamma sequences detected in Brazil was a P.1.4 lineage variant sampled in the Amazonas in October 2021 (EPI_ISL_5621224) that harbors 20 S mutations (eight more than the parental P.1 lineage), including several in common with (Delta144, Q498R, N501Y, H655Y, and N679K) or like (T95N, K417T, and E484K) those detected in Omicron.
Emergency SARS-CoV-2 Variants of Concern: Novel Multiplex Real-Time RT-PCR Assay for Rapid Detection and Surveillance.
Result: Using the LightCycler 96 Thermocycler (Roche, Mannheim, Germany), the LoD obtained from the 20 replicate tests was 30 copies/muL for the mutant
Table: E484K
Figure: (A) VOI (K417T and P681H) and VOC (DeltaHV69-79, K417N, L452R, E484K, E484Q, N501Y, and P681R).
Figure: Yellow, DeltaHV69-70; light violet, K417N and 417T; green, L452R; brown, E484K and E484Q; gray, N5011Y; and pink, P681H and P681R.
Characterization of Two Heterogeneous Lethal Mouse-Adapted SARS-CoV-2 Variants Recapitulating Representative Aspects of Human COVID-19.
Discussion: In addition, several studies have confirmed that N439K, S477N, E484K, and N501Y mutation resulted in immune escape by developing resistance to the SARS-CoV-2 neutralizing antibody.
Classical and Next-Generation Vaccine Platforms to SARS-CoV-2: Biotechnological Strategies and Genomic Variants.
PMID: 35206580
2022
International journal of environmental research and public health
Conclusion: In laboratory tests, SARS-CoV-2 variants containing the L452R or E484K substitution in the spike protein cause a marked reduction in susceptibility to bamlanivimab and possibly also etesevimab and casirivimab.
Introduction: About 14/30 substitutions including N501Y and E484K are presented in other variants as the Beta, Gamma, Theta, and Omicron.
Introduction: B.1.1.351 has three genomic variants (K417N, E484K, and N501Y) in the RBD and is more transmissible than the parental strain; it shows an increased viral load and resistance to neutralization by antibodies generated either by natural infection or by vaccination.
Introduction: Both variants (50
Establishment of a Rapid Typing Method for Coronavirus Disease 2019 Mutant Strains Based on PARMS Technology.
Introduction: A study found that compared with the USAWA1/2020 strain, the variant strain containing the E484K mutation was vaccinated with two doses of SARS-CoV-2 BNT162b2 vaccine, the vaccine-induced immunity level was still lower.
Introduction: Relevant data show that for strains containing E484K variants, the antibody titer induced by the vaccine should be increased.
Introduction: The E484K mutation in the Beta and Gamma variants may use the receptor binding domain on the S protein to escape neutralizing antibodies caused by previous infections or vaccination.
Introduction: The key mutation sites N501Y, E484K, L452R, etc.
Introduction: These evidences preliminarily indicate that the
Comparative Analysis of Five Multiplex RT-PCR Assays in the Screening of SARS-CoV-2 Variants.
Abstract: Then, specimens were screened for the detection of L452R, W152C, K417T, K417N, E484Q, E484K and N501Y mutations using the SARS-CoV-2 Variants II Assay Allplex, UltraGene Assay SARS-CoV-2 452R & 484K & 484Q Mutations V1,
Result: In particular, in the former assay, L452R mutations were observed for two samples positive for E484K.
Result: In the latter assay, one sample was identified as wild type instead of Delta (B.1.617.2) for the L452R mutation, and in another sample, Gamma (P.1) was only positive for N501Y with a drop-out for the E484K mutation.
Genomic Diversity of SARS-CoV-2 in Algeria and North African Countries: What We Know So Far and What We Expect?
Discussion: Comparative genomic analysis of SARS-CoV-2 genomes revealed multiple crucial mutations to the Spike gene including K417N, K417T, E484K, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H, which may aggravate the severity of SARS-CoV-2 more than the wild type variant, and potentially raise the concern of vaccine efficacy against novel strains.
Detection and isolation of SARS-CoV-2 Eta variant from the international travelers and local residents of India.
Abstract: The detection of this variant with lethal E484K mutation across the globe and India necessitates persistent genomic surveillance of the SARS-CoV-2 variants, which would aid in taking preventive action.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Process biochemistry (Barking, London, England)
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