Discussion: In addition, several studies have confirmed that N439K, S477N, E484K, and N501Y mutation resulted in immune escape by developing resistance to the SARS-CoV-2 neutralizing antibody.
Classical and Next-Generation Vaccine Platforms to SARS-CoV-2: Biotechnological Strategies and Genomic Variants.
PMID: 35206580
2022
International journal of environmental research and public health
Conclusion: In laboratory tests, SARS-CoV-2 variants containing the L452R or E484K substitution in the spike protein cause a marked reduction in susceptibility to bamlanivimab and possibly also etesevimab and casirivimab.
Introduction: About 14/30 substitutions including N501Y and E484K are presented in other variants as the Beta, Gamma, Theta, and Omicron.
Introduction: B.1.1.351 has three genomic variants (K417N, E484K, and N501Y) in the RBD and is more transmissible than the parental strain; it shows an increased viral load and resistance to neutralization by antibodies generated either by natural infection or by vaccination.
Introduction: Both variants (50
Establishment of a Rapid Typing Method for Coronavirus Disease 2019 Mutant Strains Based on PARMS Technology.
Introduction: A study found that compared with the USAWA1/2020 strain, the variant strain containing the E484K mutation was vaccinated with two doses of SARS-CoV-2 BNT162b2 vaccine, the vaccine-induced immunity level was still lower.
Introduction: Relevant data show that for strains containing E484K variants, the antibody titer induced by the vaccine should be increased.
Introduction: The E484K mutation in the Beta and Gamma variants may use the receptor binding domain on the S protein to escape neutralizing antibodies caused by previous infections or vaccination.
Introduction: The key mutation sites N501Y, E484K, L452R, etc.
Introduction: These evidences preliminarily indicate that the
Comparative Analysis of Five Multiplex RT-PCR Assays in the Screening of SARS-CoV-2 Variants.
Method: Each assay can differentiate among some of these viral variants by identifying typical mutations: Alpha (B.1.1.7) by means of N501Y probe and, in some sequences, E484K probe; Beta (B.1.351) with N501Y, E484K and K417N probes; Gamma (P.1) with N501Y, E484K and K417T probes; Delta (B.1.617.2) through L452R probe and, in some sequences but not all, together with K417N probe; Iota (B.1.526) with E484K probe and, in some sequences, L452R probe; and Kappa (B.1.617.1) through L452R and E484Q probes.
Genomic Diversity of SARS-CoV-2 in Algeria and North African Countries: What We Know So Far and What We Expect?
Discussion: Comparative genomic analysis of SARS-CoV-2 genomes revealed multiple crucial mutations to the Spike gene including K417N, K417T, E484K, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H, which may aggravate the severity of SARS-CoV-2 more than the wild type variant, and potentially raise the concern of vaccine efficacy against novel strains.
Detection and isolation of SARS-CoV-2 Eta variant from the international travelers and local residents of India.
Abstract: The detection of this variant with lethal E484K mutation across the globe and India necessitates persistent genomic surveillance of the SARS-CoV-2 variants, which would aid in taking preventive action.
Whole genome sequence analysis showing unique SARS-CoV-2 lineages of B.1.524 and AU.2 in Malaysia.
Result: Taken together, the missense mutations, L18F, N501Y, A701V and G1223C seem to have increased the binding affinity of the spike protein, whereas mutations D80A, D215G, K417N, N439K, E484K and A688S had the opposite effect.
Discussion: Higher infectivity of the SARS-CoV-2 variants is associated with increased in binding affinity between spike protein and ACE2 due to K417N, E484K, N439K and N501Y mutations in the
Spike Gene Evolution and Immune Escape Mutations in Patients with Mild or Moderate Forms of COVID-19 and Treated with Monoclonal Antibodies Therapies.
Method: For each sample, screening of the E484K mutation was performed with a real-time PCR (TIB MOLBIOL VirSNiP Assay 484K, ref: 53-0789).
Result: At baseline, the E484K mutation was detected in only one sample issued from patient P6, and Sanger sequencing confirmed the presence of a Variant of Concern (VOC), the Gamma (20J/501Y.V3) variant.
Discussion: Our results also corroborate in vitro results observed under selective pressure from bamlanivimab, with the emergence of immune escape mutations, such as E484K and Q493R/K.
Characterization of a Broadly Neutralizing Monoclonal Antibody against SARS-CoV-2 Variants.
Introduction: It has been reported that several mutations in the SARS-CoV-2 RBM, such as N439K, L452R, S477N, T478K, E484K, S494P, N501Y, and A502S, have increased the infectivity and stability of SARS-CoV-2.
Discussion: However, a reduced binding efficacy was observed against variants (Beta, Kappa, and Gamma) containing amino acid mutation at E484K or Q (Table 1).
Rapid SARS-CoV-2 Intra-Host and Within-Household Emergence of Novel Haplotypes.
Introduction: Another mutation of note is E484K, which is found in the Beta and Gamma variants of concern and is related to a decrease in the susceptibility to monoclonal antibodies and convalescent plasma.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Frontiers in immunology
Browser Board
Co-occurred Entities
Filtrator
ViMRT