Abstract: Thus, the spike Q677H substitution should be considered as another example of convergent evolution, as it is the case of spike substitutions L18F, E484K, L452R, and N501Y which also independently appeared in various lineages.
Introduction: The variants recently considered to be of greatest concern are those carrying amino acid substitutions N501Y and/or E484K within the spike protein, as they have increased affinity for the ACE2 cellular receptor, decreased sensitivity to neutralising antibodies, and may escape the immune responses elicited by the vaccines currently used in Western countries.
A monoclonal antibody that neutralizes SARS-CoV-2 variants, SARS-CoV, and other sarbecoviruses.
Introduction: As an example, a single mutation, E484 K, found in several variants could knock out a class of antibodies binding the receptor binding motif (RBM) on the viral spike.
Result: Here we assessed 2-36 activity on more variants, including pseudoviruses representing the combination of key spike mutations of B.1.427/B.1.429, R.1, B.1.1.1, B.1.525, B.1.617.1, B.1.617.2 and B.1.1.7 with E484 K, as well as many pseudoviruses with single spike mutations which are naturally circulating in COVID-19 patients with high frequency and located in the N-terminal domain, RBD, or S2.
Enzymatic Beacons for Specific Sensing of Dilute Nucleic Acid.
Discussion: There was another sequence sampled from Shiraz in Oct 2020 that contained five specific mutations (D138Y, D614G, E484K, K417T, N501Y) as indicator of the Gamma variant (GR/501Y.V3 (P.1+P.1.x)) and was clustered along with the representative Gamma variant spike sequence in phylogenetic tree.
Emergence of a novel SARS-CoV-2 Pango lineage B.1.1.526 in West Bengal, India.
PMID: 34896696
2022
Journal of infection and public health
Abstract: CONCLUSIONS: The D614G, P618H and
Table: E484K
Figure: (C) The sub-branch of the new lineage (B.1.1.526) illustrating 27 strains with additional S: E484K mutation.
Discussion: Interestingly, among 129 SARS-CoV-2 strains that encompass the novel lineage B.1.1.526, 27 strains harbor E484K mutation, which described to have role in immune evasion.
Discussion: Therefore, the novel lineage B.1.1.526 having D614G, P681H, V1230L and E484K mutations in the S glycoprotein is expected to have increased infectivity, high transmissibility, and enhanced immune evasion properties.
Efficacy of mRNA, adenoviral vector, and perfusion protein COVID-19 vaccines.
Introduction: In the spike protein of the B.1.1.28 strain, there are eleven mutations, including L18F, T20N, P26S, D138Y, R190S, H655Y, T1027I, D614G, K417T, E484K, and N501Y.
Introduction: Strain B.1.351 contains nine mutations within spike protein, including the L18F, D80A, D215G, R246I, K417N, Table: E484K
Surveillance of SARS-CoV-2 variants of concern by identification of single nucleotide polymorphisms in the spike protein by a multiplex real-time PCR.
PMID: 34822912
2022
Journal of virological methods
Method: Details of the primers and duel labelled probes used are previously described and used to detect the presence of the following spike protein mutations: N501Y, E484 K and L452R with K417 as reference: N501Y, E484 K, K417 and L452R.
Discussion: Furthermore, it would also help to monitor mutations of concern such as the E484 K that could arise in the delta variant itself.
Discussion: In fact, we have recently detected the E484 K mutation in the delta variant itself and the use of this multi-plex RT-q PCR would help us to further monitor the spread of this variants, in a larger sample cohort.
Discussion: Therefore, this multiplex real-time PCR approach woul
Severe breakthrough COVID-19 with a heavily mutated variant in a multiple myeloma patient 10 weeks after vaccination.
PMID: 34909634
2022
Clinical infection in practice
Discussion: The E484Q mutation in the RBD, not present in the original B.1.628 isolate, is also present in the kappa variant of interest and thought to confer immune evasion similar to E484K, which alone reduced neutralizing titers by a median of 2.8-fold among recipients of mRNA vaccines for COVID-19.
Reduced sensitivity of the SARS-CoV-2 Lambda variant to monoclonal antibodies and neutralizing antibodies induced by infection and vaccination.
Discussion: In addition, our previous studies revealed that escape of the L452R and E484 K mutants from mAbs frequently occurred in conjunction.
SARS-CoV-2 infections in mRNA vaccinated individuals are biased for viruses encoding spike E484K and associated with reduced infectious virus loads that correlate with respiratory antiviral IgG levels.
Abstract: The titers and NAbs of secretory IgA (SIgA)/IgA, secretory IgM (IgM)/IgM, and IgG against SARS-CoV-2 RBD with mutations N501Y or E484K were measured by using ELISA and a surrogate virus neutralization assay.
Abstract: The titers of SIgM/IgM and the inhibition of NAbs were higher against the mutation E484K than N501Y.
SARS_CoV_2 mutation literature information.
PMID: 
2022
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