Abstract: Vaccine breakthrough infections were more commonly associated with circulating antibody-resistant variants carrying >=1 mutation associated with decreased antibody neutralization (L452R/Q, E484K/Q and/or F490S) than infections in unvaccinated individuals (78% versus 48%, P = 1.96 x 10-8).
Modeling SARS-CoV-2 spike/ACE2 protein-protein interactions for predicting the binding affinity of new spike variants for ACE2, and novel ACE2 structurally related human protein targets, for COVID-19 handling in the 3PM context.
Abstract: By using our pipeline, we built 3D comparative models of the SARS-CoV-2 spike RBD/ACE2 protein complexes for the VoC B.1.1.7-United Kingdom (carrying the mutations of concern/interest N501Y, S494P, E484K at the RBD), P.1-Japan/Brazil (RBD mutations: K417T, E484K, N501Y), B.1.351-South Africa (RBD mutations: K417N, E484K, N501Y), B.1.427/B.1.429-California (RBD mutations: L452R), the B.1.141 (
Omicron escapes the majority of existing SARS-CoV-2 neutralizing antibodies.
Introduction: 6c, 7c), such as the E484K mutation found in Beta.
Figure: b, E484K/E484A escape scores and corresponding E484K pseudovirus neutralizing IC50 fold change compared to D614G pseudovirus of antibodies within epitope group C.
Severe breakthrough COVID-19 with a heavily mutated variant in a multiple myeloma patient 10 weeks after vaccination.
PMID: 34909634
2022
Clinical infection in practice
Discussion: The E484Q mutation in the RBD, not present in the original B.1.628 isolate, is also present in the kappa variant of interest and thought to confer immune evasion similar to E484K, which alone reduced neutralizing titers by a median of 2.8-fold among recipients of mRNA vaccines for COVID-19.
Integrin/TGF-beta1 inhibitor GLPG-0187 blocks SARS-CoV-2 Delta and Omicron pseudovirus infection of airway epithelial cells which could attenuate disease severity.
Method: Hyeryun Choe, The Scripps Research Institute, Jupiter, FL), or S protein with N501Y, E484K, N501Y+E484K or N501Y+E484K+K417N mutations.
Result: In addition to D614G, several other SARS-CoV-2 pseudovirus variants were also tested including D614, N501Y, E484K, N501Y + E484K (N+E), N501Y + E484K + K417N (NEK), R685A.
SARS-CoV-2 variants with reduced infectivity and varied sensitivity to the BNT162b2 vaccine are developed during the course of infection.
Introduction: Interestingly, despite a much lower mutation rate, recent studies showed that SARS-CoV-2 has the potential to escape neutralizing antibodies, namely by introducing the E484K, N501Y or K417N/E484K spike mutations.
But Mouse, You Are Not Alone: On Some Severe Acute Respiratory Syndrome Coronavirus 2 Variants Infecting Mice.
Abstract: This effect could be enhanced by mutations in positions 417, 484, and 493 (especially K417N, E484K, Q493K, and Q493R), and to a lesser extent by mutations in positions 486 and 499 (such as F486L and P499T).
A short plus long-amplicon based sequencing approach improves genomic coverage and variant detection in the SARS-CoV-2 genome.
Abstract: Analysis showed 26 SARS-CoV-2 lineage defining mutations including 4 known variants of concern K417N, E484K, N501Y, P618H in spike gene.
Result: K417N, E484K, N501Y, P618H and variants of interest i.e.
Result: Long-amplicon data captured 20 key lineage defining mutations including spike gene variants of concern K417N, E484K, N501Y and P618H (S3 File).
SARS-CoV-2 multiplex RT-PCR to detect variants of concern (VOCs) in Malaysia, between January to May 2021.
PMID: 35026305
2022
Journal of virological methods
Abstract: We evaluated Allplex SARS-CoV-2 Master Assay and Variants I Assay to detect HV69/70 deletion, Y144 deletion, E484K, N501Y, and P681H spike mu
Result: All 77 spike variants had detectable spike mutations using the Variants I Assay, comprising 18 with HV69/70 deletion and N501Y mutation confirmed as lineage B.1.1.7 using NGS, and 59 positive for E484K and N501Y mutations confirmed as B.1.351 by NGS (Table 1).
Result: Similarly, the Variants I Assay showed similar Ct values of RdRp and HV69/70 deletion/E484K/N501Y, with average deviation of 1.0 cycles.
Aggregation of high-frequency RBD mutations of SARS-CoV-2 with three VOCs did not cause significant antigenic drift.
Introduction: Another important amino acid mutant is E484K, reaching more than 5.7% of total sequences.
Introduction: As of March 2021, the 15 most commonly observed mutations in the RBD were as follows: V367F, P384L, K417N, N439K, L452R, Y453F, S477N, S477R, T478K, E484K, S494P, N501T, N501Y, A520S, and A522S, which were located at 13 sites in the RBD.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Nature microbiology
Browser Board
Co-occurred Entities
Filtrator
ViMRT