A year living with SARS-CoV-2: an epidemiological overview of viral lineage circulation by whole-genome sequencing in Barcelona city (Catalonia, Spain).
Abstract: But some mutations of concern, such as E484K from B.1.351 and P.1 lineages are currently under monitoring, together with those observed in the receptor-binding domain or N-terminal domain, such as L452R and T478K from B.1.617.2 lineage.
Result: Other mutations of concern such as Discussion: Nonetheless, the prevalence of E484K mutation among other lineages also increased in many countries due to its benefits for SARS-CoV-2, not only for its major transmissibility, but also evading antibody neutralization from host immune response.
Discussion: The same activity is described for E484K substitution, shared by B.1.351 and P.1 lineages, and reported during the initial weeks of the third wave in our study, as in other European regions.
Computational modelling of potentially emerging SARS-CoV-2 spike protein RBDs mutations with higher binding affinity towards ACE2: A structural modelling study.
PMID: 34979405
2022
Computers in biology and medicine
Introduction: For instance, we previously reported that the E484K variant could be more devastating than anyone else and thus emerged as a Delta variant which is more lethal and contagious.
Introduction: These variants, such as B.1.17 (Alpha variant) harbouring a mutation N501Y reported in the UK, D614G
Result: Among the reported mutations, N501Y, E484K, K417 N, E484Q and L452R elevate the pathogenicity scale.
Result: Recently our group also reported that E484K mutation alone in the RBD domain may increase Spike variant binding to ACE2 and hence enhance virus infectivity and transmissibility.
Analysis of SARS-COV2 spike protein variants among Iraqi isolates.
Introduction: Beta variant (B.1.351) lineage is another variant of concern (VOC), which was first reported in South Africa in October 2020, and characterized by eight mutations in the spike protein region, including K417N, E484K, and N501Y.
Introduction: There are other variants, such as P.1 (Gamma) lineage (20J/501Y.V3) which is mainly spread in Brazil, carrying 17 mutations; three of them are located in the spike protein regions (K417T, E484K, and N501Y).
Inadequate design of mutation detection panels prevents interpretation of variants of concern: results of an external quality assessment for SARS-CoV-2 variant detection.
PMID: 34751522
2022
Clinical chemistry and laboratory medicine
Abstract: For five samples containing the VoC Alpha + E484K, Beta, Gamma, Delta, or B.1.1.318 (as a variant of interest), 848 results for SARS-2-CoV mutation detection were reported, 824 (97.2%, range per sample 88-100%) of which were correct.
Engineered small extracellular vesicles displaying ACE2 variants on the surface protect against SARS-CoV-2 infection.
PMID: 34982509
2022
Journal of extracellular vesicles
Abstract: Furthermore, engineered sEVs inhibit the entry of wild-type (WT), the globally dominant D614G variant, Beta (K417N-E484K-N501Y) variant, and Delta (L452R-T478K-D614G) variant SARS-CoV-2 pseudovirus, and protect against authentic SARS-CoV-2 and Delta varia
Introduction: Further, pseudoviruses of the B1.351 lineage, also known as Beta variant, containing S protein receptor-binding domain (RBD) mutations, including N501Y, E484K, and K417N, exhibited increased resistance to neutralization by therapeutic monoclonal antibodies and convalescent plasma (Wibmer et al.,).
Developing an Amplification Refractory Mutation System-Quantitative Reverse Transcription-PCR Assay for Rapid and Sensitive Screening of SARS-CoV-2 Variants of Concern.
Introduction: Another study revealed that, due to mutations K417N, E484K, and N501Y in the spike protein of B.1.351, convalescent and some vaccine sera might only offer limited protection against B.1.351.
Introduction: These B.1.351 strains were about 9.4-fold more refractory to neutralization by convalescent plasma, which was largely due to the E484K mutation in the spike protein.
Result: It is worth noting that the hot spot amino acid substitutions currently of interest, such as N501Y, E484K, and P681R, were not unique or specific mutations able to differentiate the VOCs.
Identification of genotypic variants and its proteomic mutations of Brazilian SARS-CoV-2 isolates.
Introduction: Because the mutants of B.1.351 exert the highest possibility of immune evasion so far, its RBD was chosen as the representative immunogen for E484K/Q mutant lineages.
Introduction: Given that the B.1.351 and P.1 strains harbor K417N/T, E484K, and N501Y mutations in the RBD domain, B.1.526 harbors E484K, and B.1.617.1 harbors L452R and E484Q (Figure S4C), we wanted to develop a D614G- and E484K/Q-specific bivalent vaccine.
Introduction: Importantly, compared with the D614 and D614G lineages, most of these variants, especially thos
Emergence of two distinct variants of SARS-CoV-2 and an explosive second wave of COVID-19: the experience of a tertiary care hospital in Pune, India.
Result: In addition, instead of the E484K mutation seen in strains from Brazil and South Af
Table: E484K
Discussion: In two different sequences, E484K was present without any change at position 452 (Table 2).
Discussion: Interestingly, two Indian strains (CD211295-MW969753, CD210761-MZ021503) had a combination of two substitutions, L18F and E484K.
Discussion: The E484K variant is of serious concern because of its resistance to some therapeutic monoclonal antibodies and decreased in vitro neutralization by the serum samples from patients infected with the original SARS-CoV-2.
Discussion: The role of simultaneous L18F and E484K mutations in virus transmission needs to be evaluated.
Haplotype distribution of SARS-CoV-2 variants in low and high vaccination rate countries during ongoing global COVID-19 pandemic in early 2021.
PMID: 34848355
2022
Infection, genetics and evolution
Introduction: Emerging SARS-CoV-2 variants in early 2021, including B.1.1.7/B.1.1.7 + E484K lineage (United Kingdom), B.1.351 lineage (South Africa), P.1 lineage (Brazil), and B.1.427/B.1.429 lineage (California) has rapidly become dominant in domestic and arousing global concerns.
Table: E484K
Discussion: Notably, sub-haplotypes 2A_3 and 2B_1 had the mutations at K417T, and E484K in the spike was a small proportion in the high partly vaccinated rate group in May 2021, which was found to decrease neutralization of human immune serum and cause severe disease even in patients that have been previously infected.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Emerging microbes & infections
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