Inadequate design of mutation detection panels prevents interpretation of variants of concern: results of an external quality assessment for SARS-CoV-2 variant detection.
PMID: 34751522
2022
Clinical chemistry and laboratory medicine
Abstract: For five samples containing the VoC Alpha + E484K, Beta, Gamma, Delta, or B.1.1.318 (as a variant of interest), 848 results for SARS-2-CoV mutation detection were reported, 824 (97.2%, range per sample 88-100%) of which were correct.
Integrin/TGF-beta1 inhibitor GLPG-0187 blocks SARS-CoV-2 Delta and Omicron pseudovirus infection of airway epithelial cells which could attenuate disease severity.
Method: Hyeryun Choe, The Scripps Research Institute, Jupiter, FL), or S protein with N501Y, E484K, N501Y+E484K or N501Y+E484K+K417N mutations.
Result: In addition to D614G, several other SARS-CoV-2 pseudovirus variants were also tested including D614, N501Y, E484K, N501Y + E484K (N+E), N501Y + E484K + K417N (NEK), R685A.
SARS-CoV-2 variants with reduced infectivity and varied sensitivity to the BNT162b2 vaccine are developed during the course of infection.
Introduction: Interestingly, despite a much lower mutation rate, recent studies showed that SARS-CoV-2 has the potential to escape neutralizing antibodies, namely by introducing the E484K, N501Y or K417N/E484K spike mutations.
But Mouse, You Are Not Alone: On Some Severe Acute Respiratory Syndrome Coronavirus 2 Variants Infecting Mice.
Abstract: This effect could be enhanced by mutations in positions 417, 484, and 493 (especially K417N, E484K, Q493K, and Q493R), and to a lesser extent by mutations in positions 486 and 499 (such as F486L and P499T).
Identification of genotypic variants and its proteomic mutations of Brazilian SARS-CoV-2 isolates.
Abstract: Analysis showed 26 SARS-CoV-2 lineage defining mutations including 4 known variants of concern K417N, E484K, N501Y, P618H in spike gene.
Result: K417N, E484K, N501Y, P618H and variants of interest i.e.
Result: Long-amplicon data captured 20 key lineage defining mutations including spike gene variants of concern K417N, E484K, N501Y and P618H (S3 File).
SARS-CoV-2 multiplex RT-PCR to detect variants of concern (VOCs) in Malaysia, between January to May 2021.
PMID: 35026305
2022
Journal of virological methods
Abstract: We evaluated Allplex SARS-CoV-2 Master Assay and Variants I Assay to detect HV69/70 deletion, Y144 deletion, E484K, N501Y, and P681H spike mu
Result: All 77 spike variants had detectable spike mutations using the Variants I Assay, comprising 18 with HV69/70 deletion and N501Y mutation confirmed as lineage B.1.1.7 using NGS, and 59 positive for E484K and N501Y mutations confirmed as B.1.351 by NGS (Table 1).
Result: Similarly, the Variants I Assay showed similar Ct values of RdRp and HV69/70 deletion/E484K/N501Y, with average deviation of 1.0 cycles.
Aggregation of high-frequency RBD mutations of SARS-CoV-2 with three VOCs did not cause significant antigenic drift.
Introduction: Another important amino acid mutant is E484K, reaching more than 5.7% of total sequences.
Introduction: As of March 2021, the 15 most commonly observed mutations in the RBD were as follows: V367F, P384L, K417N, N439K, L452R, Y453F, S477N, S477R, T478K, E484K, S494P, N501T, N501Y, A520S, and A522S, which were located at 13 sites in the RBD.
A 48-Year-Old Immunocompetent Female Resident of Southern Florida with Confirmed Reinfection with P.1 (Gamma) Variant of SARS-CoV-2.
PMID: 35041639
2022
The American journal of case reports
Abstract: Whole-genome sequencing (WGS) of viral RNA from the patient's second infection confirmed that the viral strain was P.1 variant containing the E484K spike protein substitution.
Conclusion: WGS of viral RNA from the patient's second infection showed the viral strain to be P.1 variant containing the E484K spike protein substitution, which is considered a substitution of therapeutic concern (SOTC) by the CDC.
Unique Evolution of SARS-CoV-2 in the Second Large Cruise Ship Cluster in Japan.
Introduction: This extensive molecular surveillance of the virus has enabled researchers to identify critical mutations that might contribute to higher transmissibility or immune escape, such as D614G, N501Y, and E484K amino acid substitutions in the spike (S) protein.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Clinical chemistry and laboratory medicine
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