Introduction: As an example, a single mutation, E484 K, found in several variants could knock out a class of antibodies binding the receptor binding motif (RBM) on the viral spike.
Result: Here we assessed 2-36 activity on more variants, including pseudoviruses representing the combination of key spike mutations of B.1.427/B.1.429, R.1, B.1.1.1, B.1.525, B.1.617.1, B.1.617.2 and B.1.1.7 with E484 K, as well as many pseudoviruses with single spike mutations which are naturally circulating in COVID-19 patients with high frequency and located in the N-terminal domain, RBD, or S2.
Genomic characterization of the dominating Beta, V2 variant carrying vaccinated (Oxford-AstraZeneca) and nonvaccinated COVID-19 patient samples in Bangladesh: A metagenomics and whole-genome approach.
Abstract: Noteworthily, the receptor binding domain (RBD) region of S protein of all the isolates harbored similar substitutions including K417N, E484K, and N501Y that signify the Beta, while D614G, D215G, D80A, A67V, L18F, and A701V substitutions were commonly found in the non-RBD region of Spike proteins.
Structural and functional insights into the major mutations of SARS-CoV-2 Spike RBD and its interaction with human ACE2 receptor.
PMID: 34955621
2022
Journal of King Saud University. Science
Introduction: Due to the E484K substitution, the 501Y.V2 variant is more resistant to multiple monoclonal antibodies, convalescent plasma, and vaccine sera, whereas the N501Y substitution enhanced the affinity to human ACE2 and infectivity.
Introduction: Substitution mutations like D614G, N501Y, Y453F, N439K/R, P681H, K417N/T, and E484K, as well as deletion mutations like DeltaH69/V70 and Delta242-244 are the most common in the spike protein.
Introduction: The E484K mutation is present in the Beta variants, whereas the E484K and
Surveillance of SARS-CoV-2 variants of concern by identification of single nucleotide polymorphisms in the spike protein by a multiplex real-time PCR.
PMID: 34822912
2022
Journal of virological methods
Method: Details of the primers and duel labelled probes used are previously described and used to detect the presence of the following spike protein mutations: N501Y, E484 K and L452R with K417 as reference: N501Y, E484 K, K417 and L452R.
Discussion: Furthermore, it would also help to monitor mutations of concern such as the E484 K that could arise in the delta variant itself.
Discussion: In fact, we have recently detected the E484 K mutation in the delta variant itself and the use of this multi-plex RT-q PCR would help us to further monitor the spread of this variants, in a larger sample cohort.
Discussion: Therefore, this multiplex real-time PCR approach woul
Isolation of SARS-CoV-2 B.1.1.28.2 (P2) variant and pathogenicity comparison with D614G variant in hamster model.
PMID: 34959053
2022
Journal of infection and public health
Introduction: During January 2021, lineage P.1, also known as Gamma variant, a VOC with 17 amino acid changes which includes N501Y, E484K, and K417N on the spike protein; ORF1b deletion outside the <
Result: The amino acid substitutions detected in both the isolates were D614G, E484K, V1176F in the spike, A119S, G204R, M234I, R203K in the N, L205V in NSP5, L71F in NSP7 and P32L in NSP 12.
Reduced sensitivity of the SARS-CoV-2 Lambda variant to monoclonal antibodies and neutralizing antibodies induced by infection and vaccination.
Discussion: In addition, our previous studies revealed that escape of the L452R and E484 K mutants from mAbs frequently occurred in conjunction.
Functional Antibodies Against SARS-CoV-2 Receptor Binding Domain Variants with Mutations N501Y or E484K in Human Milk from COVID-19-Vaccinated, -Recovered, and -Unvaccinated Women.
Abstract: The titers and NAbs of secretory IgA (SIgA)/IgA, secretory IgM (IgM)/IgM, and IgG against SARS-CoV-2 RBD with mutations N501Y or E484K were measured by using ELISA and a surrogate virus neutralization assay.
Abstract: The titers of SIgM/IgM and the inhibition of NAbs were higher against the mutation E484K than N501Y.
Longitudinal analysis of SARS-CoV-2 spike and RNA-dependent RNA polymerase protein sequences reveals the emergence and geographic distribution of diverse mutations.
PMID: 34801754
2022
Infection, genetics and evolution
Result: The RBD substitution E484K was observed in South America.
Result: first detected in New York (L5F, T95I, D253G; E484K or S477N; D614G, and A701V), appear together consistently after the week of October 4th, 2020, though the fact that many of these mutations are shared with other phylogenetic lineages makes it difficult to conclude that the variant originated at this time point.
Figure: 1 associated with the Iota variant of interest are L5F, D253G, E484K, S477N, D614G,
Comparative mutational analysis of SARS-CoV-2 isolates from Pakistan and structural-functional implications using computational modelling and simulation approaches.
PMID: 34968862
2022
Computers in biology and medicine
Result: Recently reported mutations in the spike proteins of SARS-CoV-2 from South Africa (Lys417Asn, Glu484Lys, Asn501Tyr) (501Y.V2Variant) and Brazil (Lys417Asn, Glu484Lys, Asn501Tyr) had led these strains to evade the vaccines successfully.
Computational modelling of potentially emerging SARS-CoV-2 spike protein RBDs mutations with higher binding affinity towards ACE2: A structural modelling study.
PMID: 34979405
2022
Computers in biology and medicine
Introduction: For instance, we previously reported that the E484K variant could be more devastating than anyone else and thus emerged as a Delta variant which is more lethal and contagious.
Introduction: These variants, such as B.1.17 (Alpha variant) harbouring a mutation N501Y reported in the UK, D614G
Result: Among the reported mutations, N501Y, E484K, K417 N, E484Q and L452R elevate the pathogenicity scale.
Result: Recently our group also reported that E484K mutation alone in the RBD domain may increase Spike variant binding to ACE2 and hence enhance virus infectivity and transmissibility.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Emerging microbes & infections
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