Impact of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Variant-Associated Receptor Binding Domain (RBD) Mutations on the Susceptibility to Serum Antibodies Elicited by Coronavirus Disease 2019 (COVID-19) Infection or Vaccination.
Result: Anti-RBD IgG ELISA shows that antibodies induced by infection with wild-type Wuhan SARS-CoV-2 fail to recognize the RBD mutants L452R/E484Q (variant B.1.617) and E484K (variants P.1 and B.1.351), while recognition of RBDN440K is unaltered.
Result: As shown previously, vaccination induces antibodies with broader specificity than viral infection, resulting in antibodies that showed reduced binding to RBD carrying mutation E484K while all other RBDs, including the Indian variants of concern were recognized well by the vaccine-induced immune sera (Figure 3C).
Result: Binding kinetics of RBD mutants to ACE2 show increased af
Emergence of a novel SARS-CoV-2 Pango lineage B.1.1.526 in West Bengal, India.
PMID: 34896696
2022
Journal of infection and public health
Abstract: CONCLUSIONS: The D614G, P618H and
Table: E484K
Figure: (C) The sub-branch of the new lineage (B.1.1.526) illustrating 27 strains with additional S: E484K mutation.
Discussion: Interestingly, among 129 SARS-CoV-2 strains that encompass the novel lineage B.1.1.526, 27 strains harbor E484K mutation, which described to have role in immune evasion.
Discussion: Therefore, the novel lineage B.1.1.526 having D614G, P681H, V1230L and E484K mutations in the S glycoprotein is expected to have increased infectivity, high transmissibility, and enhanced immune evasion properties.
Omicron N501Y mutation among SARS-CoV-2 lineages: Insilico analysis of potent binding to tyrosine kinase and hypothetical repurposed medicine.
PMID: 34929375
2022
Travel medicine and infectious disease
Introduction: The B.1.1.7 is reported to be more infectious with higher spreading rate and increased binding affinity to ACE2 in particular due to N501Y, compared with other mutations such as E484K.
Reduced sensitivity of the SARS-CoV-2 Lambda variant to monoclonal antibodies and neutralizing antibodies induced by infection and vaccination.
Discussion: In addition, our previous studies revealed that escape of the L452R and E484 K mutants from mAbs frequently occurred in conjunction.
SARS-CoV-2 infections in mRNA vaccinated individuals are biased for viruses encoding spike E484K and associated with reduced infectious virus loads that correlate with respiratory antiviral IgG levels.
Abstract: The titers and NAbs of secretory IgA (SIgA)/IgA, secretory IgM (IgM)/IgM, and IgG against SARS-CoV-2 RBD with mutations N501Y or E484K were measured by using ELISA and a surrogate virus neutralization assay.
Abstract: The titers of SIgM/IgM and the inhibition of NAbs were higher against the mutation E484K than N501Y.
Surveillance of SARS-CoV-2 variants of concern by identification of single nucleotide polymorphisms in the spike protein by a multiplex real-time PCR.
PMID: 34822912
2022
Journal of virological methods
Method: Details of the primers and duel labelled probes used are previously described and used to detect the presence of the following spike protein mutations: N501Y, E484 K and L452R with K417 as reference: N501Y, E484 K, K417 and L452R.
Discussion: Furthermore, it would also help to monitor mutations of concern such as the E484 K that could arise in the delta variant itself.
Discussion: In fact, we have recently detected the E484 K mutation in the delta variant itself and the use of this multi-plex RT-q PCR would help us to further monitor the spread of this variants, in a larger sample cohort.
Discussion: Therefore, this multiplex real-time PCR approach woul
Mutation profile of SARS-CoV-2 genome in a sample from the first year of the pandemic in Colombia.
PMID: 34933126
2022
Infection, genetics and evolution
Conclusion: Also, we found important mutations such as E484K<
Result: The mutations p.Lys1191Asp (K1191D), p.Glu484Lys (E484K), p.Leu18Phe (L18F), p.Pro26Ser (P26S), p.His655Tyr (H655Y), p.Asp614Gly (D614G), that along with other nucleotide substitutions constitute some of the VOC and VOI, were also identified, but not necessarily defining a complete lineage of interest.
Enhanced fitness of SARS-CoV-2 variant of concern Alpha but not Beta.
Introduction: E484K is thought to be responsible for the ability of Beta to escape neutralization by plasma from convalescent individuals.
Introduction: Beta has nine mutations in S, including N501Y, and two in the S receptor-binding domain (RBD), K417N and E484K.
Longitudinal analysis of SARS-CoV-2 spike and RNA-dependent RNA polymerase protein sequences reveals the emergence and geographic distribution of diverse mutations.
PMID: 34801754
2022
Infection, genetics and evolution
Figure: 1 associated with the Iota variant of interest are L5F, D253G, E484K, S477N, D614G, A701V).
Figure: L18F, E484K, N501Y, D614G, H655Y, and V1176F are associated with the Gamma variant of concern, and S13I, W152C, L452R are associated with the Epsilon variant of concern.
Figure: Some mutations are present in multiple variants: the RBD substitution E484K is also observed in the Beta,
SARS_CoV_2 mutation literature information.
PMID: 
2022
Clinical infectious diseases
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