Result: For N501Y, E484K and T478K, the results of Mutabind2 are contrary to the experimental phenomenon.
Result: In Table 2, the DeltaDeltaEele and DeltaDeltaEgb change in E484K mutation and resulted in an increased binding affinity.
Result: In our calculation, the E484K increased the binding free energy contribution with 3.7 kcal/mol, which is overestimated relative to the experimental data.
Result: Our calculation result also shows that the E484K mutation enhances the binding.
Result: The binding free energy values of E484K (1.9 kcal/mol), Result: and that of E484K is 1.8 kcal/mol., as shown in Figure 1b.
Molecular Dynamics and MM-PBSA Analysis of the SARS-CoV-2 Gamma Variant in Complex with the hACE-2 Receptor.
Result: A closer inspection on the specific contributions of each of the three mutations in the RBM indicate that the highest binding energy contribution variation was observed in the E484K mutation (Table 2).
Result: As expected, the G linked to mutations K417T (G = 2.32 kcal/mol) and E484K (G = -3.32 kcal/mol) has a compensatory character.
Result: In the literature, it has been suggested that the E484K mutation confers to the virus the ability to evade the humoral immune system rather than imprinting a higher affinity for the receptor.
Result: Regarding the E484K mutation, the results suggest a long-range electrostatic stabilization rather than a residue specific interaction.
Result: Specifically, in the gamma variant, a loss of affinity due to the E484K
Inhibitor screening using microarray identifies the high capacity of neutralizing antibodies to Spike variants in SARS-CoV-2 infection and vaccination.
Introduction: Similar results were obtained when testing a B.1.1.7 variant with an additional E484K mutation.
Introduction: The B.1.351 and P.1 variants contain three RBD mutations at E484K, N501Y, and K417N or
Discussion: Moreover, resistance could be enhanced with the combination of E484K/K417N mutations.
Discussion: The results are consistent to our data using monoclonal and polyclonal antibodies and the serum from convalescent patients and vaccinees (Figure 4 and 5), suggesting that N501Y/E484K/K417N mutations improve the inhibition of S-ACE2 interactions.
SARS-CoV-2 infections in mRNA vaccinated individuals are biased for viruses encoding spike E484K and associated with reduced infectious virus loads that correlate with respiratory antiviral IgG levels.
Abstract: The predominant virus variant was the Alpha (B.1.1.7), however a significant association between lineage B.1.526 and amino acid change S: E484K with positives after vaccination was noted.
Result: Spike substitution analysis showed that the S: E484K was associated with genomes of the vaccinated group (p = 0.0032.
Discussion: Genomic analysis revealed a statistically significant increased representation of lineage B.1.526 as well as the Spike amino acid change S: E484K in genomes from the vaccinated group.
Discussion: Our data showed a significant association of S: E484K with positives after full vaccination using a well-controlled analysis and a relatively large sample size in a time when t
Immune evasion and chronological decrease in titer of neutralizing antibody against SARS-CoV-2 and its variants of concerns in COVID-19 patients.
Introduction: Even therapeutic monoclonal antibodies have been reported to have reduced or lost neutralizing ability against variants with the E484K mutation.
Result: In particular, the Beta and Gamma variants, which commonly have the E484K mutation, are shifted more than the red line in the center.
Dynamic Ca(2+) sensitivity stimulates the evolved SARS-CoV-2 spike strain-mediated membrane fusion for enhanced entry.
Result: We found that Beta fusion activity is ~15% lower compared to the Alpha strain, which could be due to the lower affinity of ACE2 for B1.351 than B.1.1.7 spike variant, due to additional mutation on B.1.351 variant (K417N and E484K), consistent with a previous report.
Emergence and phenotypic characterization of the global SARS-CoV-2 C.1.2 lineage.
Method: The SARS-CoV-2 Wuhan-1 spike, cloned into pCDNA3.1, was mutated using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies) and NEBuilder HiFi DNA Assembly Master Mix (NEB) to include D614G (wild-type) or lineage defining mutations for Beta (L18F, D80A, D215G, 241-243del, K417N, E484K, N501Y, D614G<
Figure: Key mutations known/predicted to influence neutralization sensitivity (C136F and P25L, Delta144Y, Delta242L/243A, and E484K), or furin cleavage (H655Y and N679K) are indicated.
SARS-CoV-2 variants of concern alpha, beta, gamma and delta have extended ACE2 receptor host ranges.
PMID: 35377298
2022
The Journal of general virology
Abstract: All four VOCs were able to overcome a previous restriction for mouse ACE2, with demonstrable differences also seen for individual VOCs with rat, ferret or civet ACE2 receptors, changes that we subsequently attributed to N501Y and E484K substitutions within the spike RBD.
Multiple SARS-CoV-2 Variants Exhibit Variable Target Cell Infectivity and Ability to Evade Antibody Neutralization.
Method: The following variant spikes were made (SA, Alpha and mink) (all mutations listed with reference to the NCBI sequence YP_009724390.1): 2P Alpha (Delta69-70, Delta144, N501Y, A570D, D614G, P681H, T716A, S982A, D1118H, and K986P, V987P), FUR2P Alpha (Delta69-70, Delta144, N501Y, A570D, D614G, T716A, S982A, D1118H, and R682S, R685S, K
SARS_CoV_2 mutation literature information.
PMID: 
2022
Biomolecules
Browser Board
Co-occurred Entities
Filtrator
ViMRT