Introduction: Delta variants have a number of characteristic mutations in their spike protein, including single-nucleotide polymorphisms (SNPs) resulting in T19R, R158G, L452R, T478 K, D614G, P681R, and D950N, which are usually chosen as the detection targets of the molecular diagnostics for Delta genotyping.
SARS-CoV-2 spike E156G/Delta157-158 mutations contribute to increased infectivity and immune escape.
Method: All other plasmids expressing spike protein mutants such as pcDNA 3.1 bs(-) T19R, pcDNA 3.1 bs(-) T95I, pcDNA 3.1 bs(-) E156G/Delta157-158, pcDNA 3.1 bs(-) L452R, pcDNA 3.1 bs(-) E484Q, pcDNA 3.1 bs(-) E156G/Delta157-158/L452R, pcDNA 3.1 bs(-) E156G/Delta157-158/E484Q, pcDNA 3.1 bs(-) E156G/Delta157-158/L452R/E484Q, and pcDNA 3.1 bs(-) ICS-05 were generated using site-directed mutagenesis by PCR using the pcDNA 3.1 bs(-) spike D614G plasmid as the template.
Identification of a novel SARS-CoV-2 variant with a truncated protein in ORF8 gene by next generation sequencing.
Introduction: At the same time, the enhanced exposure of the RBM in the D614G variant led to increased sensitivity to neutralizing antibodies.
Introduction: By the summer of 2020, the SARS-CoV-2 S variant D614G (B.1 lineage) had supplanted the ancestral virus (strain Wuhan-1) worldwide, and structural analysis showed that D614G disrupts an interprotomer contact.
Introduction: Nonetheless, antibodies that target the S2 stalk further promoted the RBD-up conformation on the D614G spike.
Introduction: The D614G spike existed in an equilibrium where the RBD favors the up conformation prior to antibody binding.
Interaction Analysis of the Spike Protein of Delta and Omicron Variants of SARS-CoV-2 with hACE2 and Eight Monoclonal Antibodies Using the Fragment Molecular Orbital Method.
PMID: 35312321
2022
Journal of chemical information and modeling
Introduction: The S protein mutations in the alpha variant are DeltaH69/DeltaV70, Delta144/144, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H.
Introduction: The mutations of the S protein in the Delta variant are T19R, E156G, Delta157/158, L452R, T478K, D614G, P681R, and D950N.
The SARS-CoV-2 Delta variant induces an antibody response largely focused on class 1 and 2 antibody epitopes.
Introduction: We compare the specificity of Delta-elicited antibodies to those elicited by earlier SARS-CoV-2 variants, including the early 2020 (i.e., Wuhan-Hu-1 and D614G) and Beta variants.
Result: As expected, Delta breakthrough infection resulted in higher neutralizing titers against both D614G (by ~4.8-fold) and Delta (by ~3.8-fold) spikes than 2x BNT162b2 alone.
Result: As expected, the 2x BNT162b2 and Delta breakthrough plasmas most potently neutralized the D614G spike, and primary Delta infection plasmas most potently neutralized the Delta spike (Fig 6 and S7).
Result: Compared to the Wuhan-Hu-1 prototypical early 2020 virus, Delta has multiple mutations in the spike protein: T19R, Delt
SARS-CoV-2 Omicron variant: Immune escape and vaccine development.
Introduction: Previous studies illustrated that D614G reduces the binding affinity to ACE2 but enhances the protease cleavage of S1/S2, leading to higher transmissibility.
Introduction: Specifically, BA.1 and BA.2 display 20 identical spike mutations, which are G339D, S373P, S375F, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P68
Reduced DMPC and PMPC in lung surfactant promote SARS-CoV-2 infection in obesity.
Method: The mutant D614G SARS-CoV-2 Spike gene was obtained from Addgene (158075).
Result: D614G, the common mutation of the SARS-CoV-2 Spike protein in alpha, beta, gamma, and delta SARS-CoV-2 variants, has been reported to enhance virus replication and transmission.
Result: DMPC and PMPC also potently inhibited infection of SARS-CoV-2 pseudovirus harboring Spike D614G mutation in HEK293T-ACE2 and Vero E6 cells.
Result: Importantly, trimyristin treatment also inhibited D614G mutant SARS-CoV-2 infection.
Discussion: Importantly, DMPC and PMPC inhibit both wild-type and D614G mutant pseudotyped SARS-CoV-2 infe
Coronavirus Genomes and Unique Mutations in Structural and Non-Structural Proteins in Pakistani SARS-CoV-2 Delta Variants during the Fourth Wave of the Pandemic.
Result: The most common non-synonymous and indel mutations, present in all complete genome samples were spike_T478K, spike_T19R, spike_L452R, spike_F157del, spike_E156G, spike_P681R, spike_D614G, spike_R158del, and spike_G142D (Table 3).
Neutralisation Hierarchy of SARS-CoV-2 Variants of Concern Using Standardised, Quantitative Neutralisation Assays Reveals a Correlation With Disease Severity; Towards Deciphering Protective Antibody Thresholds.
Introduction: As the pandemic progressed, a number of single amino acid mutations in the Spike protein were detected, such as D614G and A222V.
Introduction: Referred to as Cluster 5 or B.1.1.298, several different groups of mutations were identified, with the most abundant population containing missense and deletion mutations on the Spike; 69/70del, Y453F and D614G.
Introduction: The D614G mutation was found to increase the density of Spike protein on virions and infectivity.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Emerging microbes & infections
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