Result: Spike protein D614G mutation favors Elastase-2 binding.
Result: The S:p.D614G might assist in rapid entry into the host cells followed by quick dissemination, and the RdRp:p:P323L may instead boost the replication by a faster RNA processing (exiting).
Result: We speculated no interlinked functional relationship between p:D614G of the S protein and p:P323L of the RdRp, two important G clade-featured co-occurring mutations (Figure 1).
Subtle differences in the pathogenicity of SARS-CoV-2 variants of concern B.1.1.7 and B.1.351 in rhesus macaques.
Introduction: Therefore, we studied the pathogenesis of B.1.1.7 and B.1.351 in rhesus macaques and compared it to a recent SARS-CoV-2 isolate containing the D614G substitution in spike that rapidly became dominant globally in March 2020 because of its increased transmissibility.
Result: All the spike proteins of the three isolates:D614G, B.1.1.7, and B.1.351:bound more efficiently to human and rhesus macaque ACE2 than the WA1 spike.
Result: Although B.1.1.7 appears to be detected in the various tissue samples more frequently than D614G and B.1.351, these differences were not statistically significant in individual tissues.
Result: Analysis of the abundance of antigen presence in each lung lobe of all animals again indicated a gradient of viral antigen abundance from
In Vitro Effect of Taraxacum officinale Leaf Aqueous Extract on the Interaction between ACE2 Cell Surface Receptor and SARS-CoV-2 Spike Protein D614 and Four Mutants.
Abstract: This could be shown for the wild type and mutant forms (D614G, N501Y, and a mix of K417N, E484K, and N501Y) in human HEK293-hACE2 kidney and A549-hACE2-TMPRSS2 lung cells.
Introduction: Here we report on the inhibitory potential of dandelion on the binding of the spike S1 protein RBD to the hACE2 cell surface receptor an
Discussion: To date, several studies indicate that the D614G viral lineage is more infectious than the D614 virus.
Discussion: We observed stronger binding of the variants D614G and N501Y to the ACE2 surface receptor of human cells, but all tested variants were sensitive to binding inhibition by T.
SARS-CoV-2 Evolution among Oncological Population: In-Depth Virological Analysis of a Clinical Cohort.
Discussion: Compared with the Wuhan reference strain, all of the samples sequenced in the present study had a single-nucleotide variation (SNV) at positions 241, 3037, 14408 and 23403, the last one corresponding to the D614G amino acid mutation on the S gene.
Discussion: It is considered that minority variants under specific selective pressure may become predominant and provide a fitness advantage, ultimately influencing the epidemic, as seems to have occurred with the D614G mutation.
Discussion: Similarly, in our study, except for D614G, no mutation or minority variants were observed in the S gene coding for the spike protein; most were observed within the ORF1ab gene, which encodes the replication complex responsible for RNA synthesis.|
Rapid Detection and Inhibition of SARS-CoV-2-Spike Mutation-Mediated Microthrombosis.
Result: Fibrin surface coverage for Lipo-S was 9.53% +- 1.46% (**p < 0.01 vs control, n = 4), similarly to the Lenti-S variant D614G which also promoted significant fibrin deposition surface coverage at 9.56% +- 3.23% (**p < 0.01 vs control, n = 4) (Figure 2a,b).
Result: Furthermore, anti-IL-6 staining was prominent in Lenti-S D614G transduced HAECs (Figure 3c).
Result: Lenti-S D614G exposure upregulated cytokines (e.g., TNF-alpha, IL-6, and IL-15) and chemokines (e.g., MCP1), together with aberrantly elevated level of endothelial markers, such as v
Result: c) Immunocytochemical analysis showed Lenti-S D614G increasing protein level of IL-6 (in red).
Evidence for retained spike-binding and neutralizing activity against emerging SARS-CoV-2 variants in serum of COVID-19 mRNA vaccine recipients.
Discussion: The least negative impact on neutralization was seen for Iota variant, which is reasonable since this isolate has very few changes in the spike protein (T95I, D253G and D614G) compared to the wild type WA1 isolate.
Prior infection with SARS-CoV-2 boosts and broadens Ad26.COV2.S immunogenicity in a variant-dependent manner.
Figure: (A) Neutralization of the SARS-CoV-2 D614G pseudovirus by plasma pre- and post-vaccination from participants with no prior infection (green, n = 19) and those infected in the first (blue, n = 20) and second waves (red, n = 19).
Figure: (B) Cross-reactive ADCC activity 28 days post-vaccina
Discussion: However, although Beta infection resulted in the preserved neutralization of D614G, we, like others, noted significant loss of activity against Delta.
Discussion: However, several studies have now characterized the neutralizing activity against VOCs, including Delta, which now dominates globally and shows approximately 4- to 6-fold reduced sensitivity to convalescent plasma, in comparison with D614G.
Discussion: Prior exposure to D614G resulted in reduced titers against both Beta and Delta, consistent with previous studies.
Emerging SARS-CoV-2 variants expand species tropism to murines.
Result: Our results showed that permissibility to mouse cells was not due to the D614G mutation as B.1 did not replicate in the nasal turbinate and lung of the inoculated mice.
Result: Since B.1.1.7, B.1.351, and P.3 all shares both the D614G and N501Y mutation in spike, we included B.1 as a control virus, which carries the D614G but not the N501Y mutation, to exclude the possibility that D614G conferred infectivity to mice.
Biological Significance of the Genomic Variation and Structural Dynamics of SARS-CoV-2 B.1.617.
Introduction: Some of the aa changes or similar ones in B.1.617 were also identified in other circulating lineages: D614G was also found in B.1 lineage, L452R in B.1.526 (Iota), and P681H in B.1.1.7 (Alpha).
Introduction: Though the amino acid (aa) changes of G142D, L452R, D614G, and P681R occurring in the
Result: We found that the B.1.617 viruses and H1 were distinguished by four non-synonymous mutations: U1355G (L452R), G1450C (E484Q), A1841G (D614G), and C2042G (P681R).
Genomic epidemiology and the role of international and regional travel in the SARS-CoV-2 epidemic in Zimbabwe: a retrospective study of routinely collected surveillance data.
Abstract: 151 (97%) of 156 had the Asp614Gly mutation in the spike protein.
Result: 151 (97%) of 156 samples contained the Asp614Gly mutation.
Result: We established the distribution and frequency of the Asp614Gly variant in SARS-CoV-2 genomes from Zimbabwe.
Discussion: The spike protein is thought to be important for entry of the virus into host cells and Asp614Gly has been implicated in increased cell entry.
Discussion: There has been considerable interest in the emergence of a variant lineage of SARS-CoV-2 with a non-synonymous mutation resulting in substitution of 614Gly in which a glycine residue was replaced by an aspartate residue at position 614 (Asp614Gly) of the spike protein.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Journal of medical virology
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