Result: Since the dominant S sequence variant seen in clinical isolates is D614G, all the SARS-CoV-2 pseudovirus variants we constructed were coupled with the D614G variant.
The Emergence of the New P.4 Lineage of SARS-CoV-2 With Spike L452R Mutation in Brazil.
Method: Next, in the set of genomes found, we verified all mutations, excluding those that define the B.1.1.28 lineage (C241T, F924F, P4715L, D614G, V1176F, R203K, R203R and G204R).
SARS-CoV-2 promotes RIPK1 activation to facilitate viral propagation.
Introduction: In addition, the SARS-CoV-2 viral variants carrying P323L mutation in NSP12 and the associated D614G mutation in spike protein were reported to be enriched in severely affected group.
The evaluation of potential global impact of the N501Y mutation in SARS-COV-2 positive patients.
Result: List of variations displayed in structure (nearest residue if in loop/termini region): H69del V70del(69) Y144del(143) N501Y A570D D614G P681H(674) T716I S982A D1118H as seen in Table 3.
Table: D614G
Dominant clade-featured SARS-CoV-2 co-occurring mutations reveal plausible epistasis: An in silico based hypothetical model.
Result: Spike protein D614G mutation favors Elastase-2 binding.
Result: The S:p.D614G might assist in rapid entry into the host cells followed by quick dissemination, and the RdRp:p:P323L may instead boost the replication by a faster RNA processing (exiting).
Result: We speculated no interlinked functional relationship between p:D614G of the S protein and p:P323L of the RdRp, two important G clade-featured co-occurring mutations (Figure 1).
Subtle differences in the pathogenicity of SARS-CoV-2 variants of concern B.1.1.7 and B.1.351 in rhesus macaques.
Introduction: Therefore, we studied the pathogenesis of B.1.1.7 and B.1.351 in rhesus macaques and compared it to a recent SARS-CoV-2 isolate containing the D614G substitution in spike that rapidly became dominant globally in March 2020 because of its increased transmissibility.
Result: All the spike proteins of the three isolates:D614G, B.1.1.7, and B.1.351:bound more efficiently to human and rhesus macaque ACE2 than the WA1 spike.
Result: Although B.1.1.7 appears to be detected in the various tissue samples more frequently than D614G and B.1.351, these differences were not statistically significant in individual tissues.
Result: Analysis of the abundance of antigen presence in each lung lobe of all animals again indicated a gradient of viral antigen abundance from
In Vitro Effect of Taraxacum officinale Leaf Aqueous Extract on the Interaction between ACE2 Cell Surface Receptor and SARS-CoV-2 Spike Protein D614 and Four Mutants.
Abstract: This could be shown for the wild type and mutant forms (D614G, N501Y, and a mix of K417N, E484K, and N501Y) in human HEK293-hACE2 kidney and A549-hACE2-TMPRSS2 lung cells.
Introduction: Here we report on the inhibitory potential of dandelion on the binding of the spike S1 protein RBD to the hACE2 cell surface receptor an
Discussion: To date, several studies indicate that the D614G viral lineage is more infectious than the D614 virus.
Discussion: We observed stronger binding of the variants D614G and N501Y to the ACE2 surface receptor of human cells, but all tested variants were sensitive to binding inhibition by T.
SARS-CoV-2 Evolution among Oncological Population: In-Depth Virological Analysis of a Clinical Cohort.
Discussion: Compared with the Wuhan reference strain, all of the samples sequenced in the present study had a single-nucleotide variation (SNV) at positions 241, 3037, 14408 and 23403, the last one corresponding to the D614G amino acid mutation on the S gene.
Discussion: It is considered that minority variants under specific selective pressure may become predominant and provide a fitness advantage, ultimately influencing the epidemic, as seems to have occurred with the D614G mutation.
Discussion: Similarly, in our study, except for D614G, no mutation or minority variants were observed in the S gene coding for the spike protein; most were observed within the ORF1ab gene, which encodes the replication complex responsible for RNA synthesis.|
Rapid Detection and Inhibition of SARS-CoV-2-Spike Mutation-Mediated Microthrombosis.
Result: Fibrin surface coverage for Lipo-S was 9.53% +- 1.46% (**p < 0.01 vs control, n = 4), similarly to the Lenti-S variant D614G which also promoted significant fibrin deposition surface coverage at 9.56% +- 3.23% (**p < 0.01 vs control, n = 4) (Figure 2a,b).
Result: Furthermore, anti-IL-6 staining was prominent in Lenti-S D614G transduced HAECs (Figure 3c).
Result: Lenti-S D614G exposure upregulated cytokines (e.g., TNF-alpha, IL-6, and IL-15) and chemokines (e.g., MCP1), together with aberrantly elevated level of endothelial markers, such as v
Result: c) Immunocytochemical analysis showed Lenti-S D614G increasing protein level of IL-6 (in red).
SARS_CoV_2 mutation literature information.
PMID: 
2021
Nature
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