Result: List of variations displayed in structure (nearest residue if in loop/termini region): H69del V70del(69) Y144del(143) N501Y A570D D614G P681H(674) T716I S982A D1118H as seen in Table 3.
Table: D614G
Dominant clade-featured SARS-CoV-2 co-occurring mutations reveal plausible epistasis: An in silico based hypothetical model.
Introduction: GISAID referred to these co-occurring mutations containing viruses as clade G (named after the spike D614G mutation) or PANGO (https://cov-lineages.org/) lineage B.1.
Introduction: The most frequently observed mutation is D614G of the S protein, which has direct roles in receptor binding and immunogenicity, thus viral immune-escape, transmission, and replication fitness.
Introduction: Yin reported that the 5'-untranslated region (5'-UTR) mutation 241C>T is co-occurring with three other mutations, 3037 C>T (NSP3: C318T), 14408C>T (RdRp:
Table: D614G
Subtle differences in the pathogenicity of SARS-CoV-2 variants of concern B.1.1.7 and B.1.351 in rhesus macaques.
Result: All the spike proteins of the three isolates:D614G, B.1.1.7, and B.1.351:bound more efficiently to human and rhesus macaque ACE2 than the WA1 spike.
Result: Although B.1.1.7 appears to be detected in the various tissue samples more frequently than D614G and B.1.351, these differences were not statistically significant in individual tissues.
Result: Analysis of the abundance of antigen presence in each lung lobe of all animals again indicated a gradient of viral antigen abundance from D614G to B.1.1.7 and B.1.351.
Result: Analysis of the histology scores assigned to each lung lobe of all animals indicated that lesions in D614G-, B.1.1.7-, and B.1.351-inoculated animals occurred on a gradient from more to less severe, respectively; these differences were statist
In Vitro Effect of Taraxacum officinale Leaf Aqueous Extract on the Interaction between ACE2 Cell Surface Receptor and SARS-CoV-2 Spike Protein D614 and Four Mutants.
Method: Then, 500 ng/mL SARS-CoV-2 Spike S1 (Trenzyme GmbH, Konstanz, Germany), spike S1 D614G, N50Y, or a mix of K417N, E484K, and N501Y (Sino Biological Europe GmbH, Eschborn, Germany)-His recombinant protein were added into each sample, and samples were further incubated for 30-60 min.
Result: After 30 s, this was 58.2 +- 28.7% for D614, 88.2 +- 4.6% for D614G, and 88 +- 1.3% for N501Y binding inhibition by T.
Result: Cell treatment with equal amounts of spike D614 and its variants D614G and N501Y confirmed a stronger binding aff
SARS-CoV-2 Evolution among Oncological Population: In-Depth Virological Analysis of a Clinical Cohort.
Discussion: Compared with the Wuhan reference strain, all of the samples sequenced in the present study had a single-nucleotide variation (SNV) at positions 241, 3037, 14408 and 23403, the last one corresponding to the D614G amino acid mutation on the S gene.
Discussion: It is considered that minority variants under specific selective pressure may become predominant and provide a fitness advantage, ultimately influencing the epidemic, as seems to have occurred with the D614G mutation.
Discussion: Similarly, in our study, except for D614G, no mutation or minority variants were observed in the S gene coding for the spike protein; most were observed within the ORF1ab gene, which encodes the replication complex responsible for RNA synthesis.|
Rapid Detection and Inhibition of SARS-CoV-2-Spike Mutation-Mediated Microthrombosis.
Result: Fibrin surface coverage for Lipo-S was 9.53% +- 1.46% (**p < 0.01 vs control, n = 4), similarly to the Lenti-S variant D614G which also promoted significant fibrin deposition surface coverage at 9.56% +- 3.23% (**p < 0.01 vs control, n = 4) (Figure 2a,b).
Result: Furthermore, anti-IL-6 staining was prominent in Lenti-S D614G transduced HAECs (Figure 3c).
Result: Lenti-S D614G exposure upregulated cytokines (e.g., TNF-alpha, IL-6, and IL-15) and chemokines (e.g., MCP1), together with aberrantly elevated level of endothelial markers, such as v
Result: c) Immunocytochemical analysis showed Lenti-S D614G increasing protein level of IL-6 (in red).
Evidence for retained spike-binding and neutralizing activity against emerging SARS-CoV-2 variants in serum of COVID-19 mRNA vaccine recipients.
Discussion: The least negative impact on neutralization was seen for Iota variant, which is reasonable since this isolate has very few changes in the spike protein (T95I, D253G and D614G) compared to the wild type WA1 isolate.
Prior infection with SARS-CoV-2 boosts and broadens Ad26.COV2.S immunogenicity in a variant-dependent manner.
Result: ADCC assays performed with the Beta and Delta variants showed no significant loss in activity when compared to D614G in the vaccine-only group (Figure 3B) or in individuals with prior infection (Figures 3B and 3C), demonstrating cross-reactive ADCC responses to VOCs.
Result: By using a SA
Discussion: However, although Beta infection resulted in the preserved neutralization of D614G, we, like others, noted significant loss of activity against Delta.
Discussion: However, several studies have now characterized the neutralizing activity against VOCs, including Delta, which now dominates globally and shows approximately 4- to 6-fold reduced sensitivity to convalescent plasma, in comparison with D614G.
Discussion: Prior exposure to D614G resulted in reduced titers against both Beta and Delta, consistent with previous studies.
Emerging SARS-CoV-2 variants expand species tropism to murines.
Result: Our results showed that permissibility to mouse cells was not due to the D614G mutation as B.1 did not replicate in the nasal turbinate and lung of the inoculated mice.
Result: Since B.1.1.7, B.1.351, and P.3 all shares both the D614G and N501Y mutation in spike, we included B.1 as a control virus, which carries the D614G but not the N501Y mutation, to exclude the possibility that D614G conferred infectivity to mice.
Biological Significance of the Genomic Variation and Structural Dynamics of SARS-CoV-2 B.1.617.
Introduction: Some of the aa changes or similar ones in B.1.617 were also identified in other circulating lineages: D614G was also found in B.1 lineage, L452R in B.1.526 (Iota), and P681H in B.1.1.7 (Alpha).
Introduction: Though the amino acid (aa) changes of G142D, L452R, D614G, and P681R occurring in the
Result: We found that the B.1.617 viruses and H1 were distinguished by four non-synonymous mutations: U1355G (L452R), G1450C (E484Q), A1841G (D614G), and C2042G (P681R).
SARS_CoV_2 mutation literature information.
PMID: 
2021
Journal of medical virology
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