Evaluating the Neutralizing Ability of a CpG-Adjuvanted S-2P Subunit Vaccine Against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Variants of Concern.
Abstract: In humans, vaccinated phase 1 subjects still showed appreciable neutralization abilities against the D614G, Alpha, and Beta variants, although neutralizing titers were significantly reduced against the Beta variant.
Abstract: The neutralizing titers of serum antibodies were assayed with pseudoviruses coated with the SARS-CoV-2 spike protein of the wild-type (WT), D614G, Alpha, or Beta variants.
Functional differences among the spike glycoproteins of multiple emerging severe acute respiratory syndrome coronavirus 2 variants of concern.
Abstract: Compared with D614G, the emerging variants exhibited an increased affinity for the receptor, ACE2, and increased ability to infect cells with low ACE2 levels.
Abstract: We compared the functional properties of spike (S) glycoproteins from the original SARS-CoV-2 strain (D614) (Wuhan, China), the globally dominant D614G strain, and emerging geographic variants: B.1.1.7 (United Kingdom), B.1.351 (South Africa), P.1 (Brazil), and B.1.1.248 (Brazil/Japan).
Result: All four variants exhibited modest (1.7-8.2-fold) increases in the estimated binding affinity for sACE2-Fc, relative to that of D614G.
Result: As was seen for the VSV pseudotypes, the authentic P.1 SARS-CoV-2 variant exhibited more S1 shedding over time at 4 C than the Table: D614G
Transmission dynamics, clinical characteristics and sero-surveillance in the COVID-19 outbreak in a population dense area of Colombo, Sri Lanka April- May 2020.
Abstract: Of the two viruses that were sequenced and were of the B.1 and B.4 lineages with one carrying the D614G mutation.
Abstract: Since the majority of those who were in this underserved settlement were not infected despite circulation of the D614G variant, it would be important to further study environmental and host factors that lead to disease severity and transmission.
Result: One of the viruses had the D614G mutation.
Table: D614G
Discussion: Despite one of the viruses carrying the D614G mutation and despite this outbreak occurring in an extremely overcrowded area, it appears that only 89/2722 living in the 6,955 square meters (0.007 km2) area of the Bandaranayaka watta were infected.
Discussion: Of the two viruses, one carried the D614G m
Mutation Y453F in the spike protein of SARS-CoV-2 enhances interaction with the mink ACE2 receptor for host adaption.
Discussion: It has been shown that the D614G substitution in the SARS-CoV-2 spike can promote virus entry into host cells and enhance infectivity as well as make the mutant virus resistant to neutralizing antibody.
Algorithm for the Quantitation of Variants of Concern for Rationally Designed Vaccines Based on the Isolation of SARS-CoV-2 Hawai'i Lineage B.1.243.
Abstract: RT-qPCR assays for the Original (D614G), Alpha and Beta variants had been previously developed and are being employed for wastewater surveillance.
Introduction: First evidence for such an evolutionary event for the SARS-CoV-2 virus occurred in April 2020, when the D614G substitution enhanced infectivity and transmission.
Introduction: The D614G mutation lineage became the dominant SARS-CoV-2 variant form.
D614G mutation and SARS-CoV-2: impact on S-protein structure, function, infectivity, and immunity.
PMID: 34755213
2021
Applied microbiology and biotechnology
Fig
Figure: D614G mutation location in the 3D model of S-glycoprotein and different conformational states of S-glycoprotein.
Figure: A 3D model of S-glycoprotein stating the location of D614G mutation.
Figure: A conceptual diagram illustrating the characteristics change of the SARS-CoV-2 variant due to the D614G mutation.
Figure: Researchers noted that D614G mutation favors the open conformation of S-glycoprotein and thus helps more interaction with the hACE2 receptor, which causes more infection and re-infection.
Figure: Schematic diagram of the location of D614G amino acids where mutation occurs in S-glycoprotein.
SARS-CoV-2 variant N.9 identified in Rio de Janeiro, Brazil.
PMID: 34755818
2021
Memorias do Instituto Oswaldo Cruz
Introduction: A novel variant of the virus emerged at the end of January 2020 in lineage B.1 presented the spike protein substitution D614G.
Changing predominant SARS-CoV-2 lineages drives successive COVID-19 waves in Malaysia, February 2020 to March 2021.
Discussion: B.1.524 viruses carry the spike mutations D614G (present in most SARS-CoV-2 viruses) and A701V, which is also found in the VOC B.1.351 and the previously designated variant of interest B.1.526 (iota).
Discussion: The nsp12 (RNA-dependent RNA polymerase) P323L mutation present in lineage B.1.524 is almost always associated with D614G; the significance of
Discussion: The mutation D614G, which has become predominant worldwide, is associated with increased cell entry, replication, and transmissibility, possibly by stabilizing the spike receptor-binding domain and enhancing binding to the host cell receptor angiotensin-converting enzyme 2.
Epidemiology of COVID-19: An updated review.
PMID: 34759999
2021
Journal of research in medical sciences
Abstract: On the other hand, among 14 detected mutations in the SARS-CoV-2 S protein that provide advantages to virus for transmission and evasion form treatment, the D614G mutation (substitution of aspartic acid [D] with glycine [G] in codon 614 was particular which could provide the facilitation of the transmission of the virus and virulence.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Clinical infectious diseases
Browser Board
Co-occurred Entities
Filtrator
ViMRT