Introduction: We compare the specificity of Delta-elicited antibodies to those elicited by earlier SARS-CoV-2 variants, including the early 2020 (i.e., Wuhan-Hu-1 and D614G) and Beta variants.
Result: As expected, Delta breakthrough infection resulted in higher neutralizing titers against both D614G (by ~4.8-fold) and Delta (by ~3.8-fold) spikes than 2x BNT162b2 alone.
Result: As expected, the 2x BNT162b2 and Delta breakthrough plasmas most potently neutralized the D614G spike, and primary Delta infection plasmas most potently neutralized the Delta spike (Fig 6 and S7).
Result: Compared to the Wuhan-Hu-1 prototypical early 2020 virus, Delta has multiple mutations in the spike protein: T19R, Delt
SARS-CoV-2 Omicron variant: Immune escape and vaccine development.
Introduction: Previous studies illustrated that D614G reduces the binding affinity to ACE2 but enhances the protease cleavage of S1/S2, leading to higher transmissibility.
Introduction: Specifically, BA.1 and BA.2 display 20 identical spike mutations, which are G339D, S373P, S375F, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P68
Conformational dynamics and allosteric modulation of the SARS-CoV-2 spike.
Introduction: At the same time, the enhanced exposure of the RBM in the D614G variant led to increased sensitivity to neutralizing antibodies.
Introduction: By the summer of 2020, the SARS-CoV-2 S variant D614G (B.1 lineage) had supplanted the ancestral virus (strain Wuhan-1) worldwide, and structural analysis showed that D614G disrupts an interprotomer contact.
Introduction: Nonetheless, antibodies that target the S2 stalk further promoted the RBD-up conformation on the D614G spike.
Introduction: The D614G spike existed in an equilibrium where the RBD favors the up conformation prior to antibody binding.
Discu
Role of the Microbiome in the Pathogenesis of COVID-19.
PMID: 35433495
2022
Frontiers in cellular and infection microbiology
Abstract: Soon after the first outbreak due to the wild-type strain in December 2019, a genetic variant D614G emerged in late January to early February 2020 and became the dominant genotype worldwide.
Conclusion: The two most commonly occurring mutations, Spike_D614G and Nsp12_P314L, were structurally modeled, which showed that these mutations had the potential to enhance viral entry and replication, respectively.
Introduction: Among these, the D614G clade was the most common and was first found in late January 2020 in China, according to a study conducted by.
Introduction: Earliest samples from the USA appeared to have been derived from China and belonged to basal or L84S
Table: D614G
Split T7 promoter-based isothermal transcription amplification for one-step fluorescence detection of SARS-CoV-2 and emerging variants.
Conclusion: Finally, (v) broad applicability was verified through the multiplex detection of the SARS-CoV-2 variants (D614G mutation) as well as through the direct detection of bacterial 16S rRNA without the need for additional nucleic acid purification.
Introduction: D614G), conferring greater infectivity and more rapid spread, have become predominant in various regions.
Introduction: Moreover, STAR was utilized for multiplex detection of the D614G mutation and N gene of SARS-CoV-2 in a single tube as well as for the direct detection of bacterial 16S rRNA without additional nucleic acid purification, thereby confirming the wide applicability of this method for nucleic acid biomarker detection.
Result: 5B indicate that G at N4 minimized the background noise in the presence of the wild-type target while generating a high fluorescence sign
Comparative genomics, evolutionary epidemiology, and RBD-hACE2 receptor binding pattern in B.1.1.7 (Alpha) and B.1.617.2 (Delta) related to their pandemic response in UK and India.
PMID: 35427787
2022
Infection, genetics and evolution
Figure: (E) Molecular association between the residues in the wild-type D614G mutation.
Figure: (F) The schematic diagram shows the molecular association between the residues of wild type D614G mutation.
Figure: (G) Molecular association between the residues in the mutant type D614G mutation.
Figure: (H) The schematic diagram shows the molecular association between the residues of mutant type D614G mutation.
Figure: The diagram shows significant mutations in B.1.1.7 (N501Y, D614G, and P681R) and B.1.617.2 (N501Y, D614G, L452R, E484Q, and P681R)
Prolonged shedding of infectious viruses with haplotype switches of SARS-CoV-2 in an immunocompromised patient.
PMID: 35430092
2022
Journal of infection and chemotherapy
Conclusion: When haplotype 1 was compared with Wuhan-Hu-1/2019 (GenBank accession number; MN908947), it had ten non-synonymous (ORF1a:Q2702H and S2981F, ORF1b:P314L, T1404 M, P1567L, and R2684I, S gene:D614G, N gene:R203K, G204R, and M234I) and five synonymous mutations.
Efficacy of vaccination and previous infection against the Omicron BA.1 variant in Syrian hamsters.
Introduction: Here, in the hamster model, we addressed these issues under controlled conditions using the Omicron variant and isolates that are no longer circulating in nature (i.e., a Wuhan-like isolate and an isolate with only a D614G spike mutation).
Antigenicity comparison of SARS-CoV-2 Omicron sublineages with other variants contained multiple mutations in RBD.
Figure: (A) Heatmap of the neutralizing activity of 24 mAbs derived from infected or vaccine immunized persons against D614G and seven variant pseudoviruses.
Figure: (B) The heatmap represents the ratio of EC50 values between seven variants and D614G reference.
Figure: Darker brown indicates higher neutralizing ac
Discussion: AM180 and 9G11 lost neutralizing activity to the other seven variants except for D614G, presumably due to the E484 mutation.
Discussion: Among them, D614G, B.1.640.1, and B.1.630 formed a cluster, C.1.2 and B.1.640.2 formed a cluster, and BA.1, BA.2, and BA.3 formed a cluster.
Discussion: Our data indicated that D614G, Alpha, Delta, and Mu were similar in immunogenicity, and these sera had significantly reduced protection against Omicron sublineages (Figure S1).
Characteristic analysis of Omicron-included SARS-CoV-2 variants of concern.
Introduction: And compared to the original strain of SARS-CoV-2, the D614G mutation makes the S protein more stable and more flexible, which makes the virus more infectious.
Introduction: For example, S
Result: In addition, Alpha, Beta, Delta, and Omicron strains had partial protruding structures at the junction of S protein and virus particles (Figure 1), which may be caused by the internal extrusion of the S protein after the D614G mutation.
Result: The K417N and D614G epitope scores have almost no change, indicating that the D614G mutation is more about changing the structure of the S protein to promote the spread of the virus.
SARS_CoV_2 mutation literature information.
PMID: 
2022
bioRxiv
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