Result: A query of all GISAID sequences from Neovison vison viruses revealed that 95.4% had mutations D614G and N501T.
Result: One of the two mink from farm A had mutations at T85I-NSP2, S1205L-NSP3, G37E-NSP9, P323L-NSP12, T91M-NSP15, D614G-spike, N501T-spike, Q57H-NS3, H182Y-NS3, and T205I-N
Common cardiac medications potently inhibit ACE2 binding to the SARS-CoV-2 Spike, and block virus penetration and infectivity in human lung cells.
Discussion: An additional limitation is that while we discovered that the alpha/beta/gamma, [D614G] mutation in the S1 protein, which is at some distance from the RBD, raises the KD for ACE2 binding to the RBD, the present data do not reveal the mechanism.
Discussion: By contrast, the potency of digitoxin and digoxin in the in vitro ACE2:RBD binding assays depended on the mutations present in the RBD, and on the presence of the [D614G] mutation in the S1 domain.
Discussion: However, it has been reported that the alpha/beta/gamma, [D614G] mutation increases spike flexibility, and spike density by 4-fivefold.
Discussion: Nonetheless, the inhibition constants f
Sequencing of SARS-CoV-2 in local transmission cases through Oxford Nanopore MinION platform from Karachi Pakistan.
PMID: 34780358
2021
Journal of infection in developing countries
Abstract: All the isolates belonged to GH clade and shared missense mutation D614G in spike protein linked to increased transmission rate worldwide.
Abstract: Another spike protein mutation A222V coexisted with D614G in the virus from the second wave of COVID-19.
Infection, recovery and re-infection of farmed mink with SARS-CoV-2.
Result: These methods were used to detect the D614G mutation, which arose in the first months of the pandemic and has become dominant worldwide as a consequence of providing higher infectivity to the virus.
CRISPR-Cas12a-Based Detection for the Major SARS-CoV-2 Variants of Concern.
Method: The SARS-CoV-2 target sequences include (i) the wild-type (WT) gene fragment of S protein (S; nucleotides [nt] 21,563 to 25,384; GenBank accession number MN908947); (ii) the mutant gene fragments of S protein, including mutations L5F, D80A, D215G, R246I, K417N, L452R/Q, Y453F, T478K, E484Q/K, N501Y, Result: Finally, crRNA-S-614D was used to distinguish the D614G mutation.
Table: D614G
A Novel Strategy for the Detection of SARS-CoV-2 Variants Based on Multiplex PCR-Mass Spectrometry Minisequencing Technology.
Abstract: Using the nucleic acid sequence of a SARS-CoV-2 nonvariant and a synthetic SARS-CoV-2 variant-carrying plasmid, a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) method based on the single-base mass probe extension of multiplex PCR amplification products was established to detect 9 mutation types in 7 mutated sites (HV6970del, N501Y, K417N, P681H, D614G, E484K, L452R, E484Q, and P681R) in the receptor-binding domain of the spike protein of SARS-CoV-2 variants.
Table: D614G
Discussion: For example, D614G and
Re-emergence of Gamma-like-II and emergence of Gamma-S:E661D SARS-CoV-2 lineages in the south of Brazil after the 2021 outbreak.
Discussion: Although the results show an increase in infectivity close to 20-fold for the D614G/L452R protein, the increase in infectivity for D614G/W152C was fourfold when compared to the S protein containing only the D614G mutation.
Discussion: Among the main mutations common between the two groups, we highlight those that occur in the S protein: S:E484K, S:N501Y and S:D614G.
Discussion: For example, the D614G substitution enhanced viral stability and infectivity, and the E484 mutation in the re
On the association between SARS-COV-2 variants and COVID-19 mortality during the second wave of the pandemic in Europe.
PMID: 34790342
2021
Journal of market access & health policy
Introduction: Besides newly identified mutations, it cannot be ignored that SARS-COV-2 variants developed during the first wave, such as 20A (mutation S:D614G), developed in February 2020 and dominant during the previous wave, can also have impact on the mortality during the second wave.
Result: A significant negative correlation between the average proportion of 20A (S:D614G) variant and second-wave deaths peak height was observed for the period from start of the se
Table: D614G
Discussion: The proportion of 20A (mutation S:D614G) variant, firstly observed in Europe during the 1st wave of the pandemic (February 2020), was found to be negatively associated with the deaths peak height considering the pre-peak phase of the second wave.
Conformational dynamics and allosteric modulation of the SARS-CoV-2 spike.
Abstract: Here, using single-molecule Forster resonance energy transfer (smFRET) imaging we report the effects of ACE2 and antibody binding on the conformational dynamics of S from the Wuhan-1 strain and the B.1 variant (D614G).
Abstract: We find that D614G modulates the energetics of the RBD position in a manner similar to ACE2 binding.
SARS_CoV_2 mutation literature information.
PMID: 
2021
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