Discussion: The least negative impact on neutralization was seen for Iota variant, which is reasonable since this isolate has very few changes in the spike protein (T95I, D253G and D614G) compared to the wild type WA1 isolate.
Prior infection with SARS-CoV-2 boosts and broadens Ad26.COV2.S immunogenicity in a variant-dependent manner.
Figure: (A) Neutralization of the SARS-CoV-2 D614G pseudovirus by plasma pre- and post-vaccination from participants with no prior infection (green, n = 19) and those infected in the first (blue, n = 20) and second waves (red, n = 19).
Figure: (B) Cross-reactive ADCC activity 28 days post-vaccina
Discussion: However, although Beta infection resulted in the preserved neutralization of D614G, we, like others, noted significant loss of activity against Delta.
Discussion: However, several studies have now characterized the neutralizing activity against VOCs, including Delta, which now dominates globally and shows approximately 4- to 6-fold reduced sensitivity to convalescent plasma, in comparison with D614G.
Discussion: Prior exposure to D614G resulted in reduced titers against both Beta and Delta, consistent with previous studies.
Emerging SARS-CoV-2 variants expand species tropism to murines.
Result: Our results showed that permissibility to mouse cells was not due to the D614G mutation as B.1 did not replicate in the nasal turbinate and lung of the inoculated mice.
Result: Since B.1.1.7, B.1.351, and P.3 all shares both the D614G and N501Y mutation in spike, we included B.1 as a control virus, which carries the D614G but not the N501Y mutation, to exclude the possibility that D614G conferred infectivity to mice.
Biological Significance of the Genomic Variation and Structural Dynamics of SARS-CoV-2 B.1.617.
Introduction: Some of the aa changes or similar ones in B.1.617 were also identified in other circulating lineages: D614G was also found in B.1 lineage, L452R in B.1.526 (Iota), and P681H in B.1.1.7 (Alpha).
Introduction: Though the amino acid (aa) changes of G142D, L452R, D614G, and P681R occurring in the
Result: We found that the B.1.617 viruses and H1 were distinguished by four non-synonymous mutations: U1355G (L452R), G1450C (E484Q), A1841G (D614G), and C2042G (P681R).
Genomic epidemiology and the role of international and regional travel in the SARS-CoV-2 epidemic in Zimbabwe: a retrospective study of routinely collected surveillance data.
Abstract: 151 (97%) of 156 had the Asp614Gly mutation in the spike protein.
Result: 151 (97%) of 156 samples contained the Asp614Gly mutation.
Result: We established the distribution and frequency of the Asp614Gly variant in SARS-CoV-2 genomes from Zimbabwe.
Discussion: The spike protein is thought to be important for entry of the virus into host cells and Asp614Gly has been implicated in increased cell entry.
Discussion: There has been considerable interest in the emergence of a variant lineage of SARS-CoV-2 with a non-synonymous mutation resulting in substitution of 614Gly in which a glycine residue was replaced by an aspartate residue at position 614 (Asp614Gly) of the spike protein.
Result: Consistent with the global trend, a high frequency of the viruses (140, 93.33%) harbored the D614G amino acid change (conferred by a SNP at nucleotide 23403) in the spike protein.
High-Throughput Next-Generation Sequencing Respiratory Viral Panel: A Diagnostic and Epidemiologic Tool for SARS-CoV-2 and Other Viruses.
Abstract: The clade for pangolin lineage B that conta
Result: Through our Nextstrain thread, it was observed that the clade for pangolin lineage B contains certain distant variants, including P4715L in ORF1ab, Q57H in ORF 3a, and S84L in ORF8 covarying with the D614G spike protein mutation, which were found to be the most prevalent in the early phase of the pandemic in the state of Georgia.
Discussion: Through our Nextstrain thread, we found that the clade for pangolin lineage B that contains certain distant variants covarying with the D614G spike protein mutation had become increasingly prevalent at the early phase of the pandemic in the state of Georgia.
SARS-CoV-2 variant with mutations in N gene affecting detection by widely used PCR primers.
Result: hCoV-19/Finland/FinD796H/2021 had a very uncommon combination of the S protein mutations, as only 0,04% (535/1204022) of complete genome sequences deposited to Gisaid at 23.4.2021 had a similar combination (S:T95I, S: 144del, S:E484K, S:D614G, S:P681H, and S:D796D&H).
Temporal-Geographical Dispersion of SARS-CoV-2 Spike Glycoprotein Variant Lineages and Their Functional Prediction Using in Silico Approach.
Result: A proportion of 99% of those novel variants contained the S-D614G mutation together with another 7 +- 5 amino acid variations within the S protein.
Result: A total of 139 unique S protein variants of beta (B.1.351) were clustered, with the majority carrying 7 consensus amino acid variations (D80A, D215G, K417N, E484K, N501Y, D614G, and A701V) and 1 deletion (amino acid 242).
Result: Among the amino acid mutations found in circulating SARS-CoV-2 variants, E484Q/K, P681H/R/L/S, PMID: 34702899
2021
Scientific reports
Discussion: A correlation of the amino acid substituted D614G associated with the G-clades and case fatality in the West African countries can only be identified at a marginal level of r = 0.28 (Supplementary Table 1).
Discussion: The D614G amino acid substitution which was the most relevant change in SARS-CoV-2 in spring 2020 became prevalent in 2021 and was boosted by continuously acquired new mutations agglomerating into new variants.
Discussion: The question if that correlates with the severity of the disease still needs to be addressed, Brufsky infers it from the higher mortality at the East Coast of USA with predominantly D614G-carrying G-clades compared to the West Coast with the predominant early clades.|mg
Discussion: found significant correlations between the percentage of D614G and case-fatality on a country by country basis.
SARS_CoV_2 mutation literature information.
PMID: 
2021
EBioMedicine
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