Abstract: In a subset, we also assessed in vitro neutralisation against the original variant (D614G WT) and the Beta variant.
Conclusion: We report a dramatic rise in SARS-CoV-2 seroprevalence from 18.5% in October 2020 to 64.9% in May 2021 in healthy blood donors as Malawi experienced the first and second COVID-19 epidemic waves, likely driven initially by the original variant (D614G WT) and then the beta variant.
Method: SARS-CoV-2-pseudotyped lentiviruses were prepared by co-transfecting the HEK 293T cell line with either the
Result: Following previous observations that antibodies elicited by the original variant (D614G WT) are less potent against the beta variant, we reasoned that differential neutralisation potency by SARS-CoV-2 antibodies could be used to estimate the predominance of specific variants driving seroprevalence.
SARS-CoV-2 Delta (B.1.617.2) Variant: A Unique T478K Mutation in Receptor Binding Motif (RBM) of Spike Gene.
Introduction: SARS-CoV-2 delta variant shares three common mutation sites, L452R, D614G, and P681R with SARS-CoV-2 kappa and B.1.617.3 variant (Table 1).
Introduction: The D614G mutation site exists all four SARS-CoV-2 variants of concern as well as all six SARS-CoV-2 variants of interest and alert.
Table: D614G
Figure: The common D614G in all ten variant (Table 1) was indicated by bold blue letter.
Introduction and rapid dissemination of SARS-CoV-2 Gamma Variant of Concern in Venezuela.
PMID: 34800714
2021
Infection, genetics and evolution
Table: D614G
Implication of SARS-CoV-2 Immune Escape Spike Variants on Secondary and Vaccine Breakthrough Infections.
Abstract: This article highlights the pressures that facilitate the rise of new SARS-CoV-2 variants and the key mutations of the viral spike protein - L452R, E484K, N501Y and D614G- that promote immune escape mechanism and warrant a cautionary point for clinical and public health responses in terms of re-infection, vaccine breakthrough infection and therapeutic values.
Introduction: D614G mutation is located outside of the RBD (Figure 1).
Introduction: D614G variant bypassed natural bottlenecks due to its selective advantages that lead to improved viral infectivity and transmissibility and reduced viral sensitivity to neutralising convalescent ser
Table: D614G
Mutation-Induced Long-Range Allosteric Interactions in the Spike Protein Determine the Infectivity of SARS-CoV-2 Emerging Variants.
Result: Along with the VOC-/VOI-specific mutations, we further examined the effects of different flexible RBD orientations (i.e., clockwise and anticlockwise movements of RBD) that can be caused by several mutations at hinge residues located close to the S1/S2 junction (e.g., D614G).
Table: D614G
A rigorous framework for detecting SARS-CoV-2 spike protein mutational ensemble from genomic and structural features.
PMID: 34806033
2021
Current research in structural biology
Result: Followed by the RBD, the SD1 (542-591 a.a.) and SD2 (592-681 a.a.) regions showed a similar trend in both the WT and D614G systems with an average RMSD of 0.61 +- 0.003 nm.
Result: In early pandemic, L5F substitution in the signal peptide was present, alongwith D614G mutation in the linker.
Result: In the D614G trajectory, it corresponded to a relatively compact state with significantly less distance between the NTD and RBD (5.97 nm and 5.82 nm, respectively) as shown in.
Result: Our analysis revealed increased stability of the D614G pro
Genome-wide identification and prediction of SARS-CoV-2 mutations show an abundance of variants: Integrated study of bioinformatics and deep neural learning.
PMID: 34812411
2021
Informatics in medicine unlocked
Introduction: For instance, the
Result: Furthermore, the monthly basis of sequence analysis revealed that D614G (spike protein), F106F (NSP3), P314L (NSP12b), and 5' UTR:241 mutations are at the top of the mutation analysis chart of every month from March 2020 to December 2020.
Discussion: In our large scale study, previously reported D614G, and P314L missense mutations are also identified as the most prevalent mutation in the viral genome.
Discussion: The P314L mutation in RdRp is associated with the D614G mutation and may favor the SARS-CoV-2 by enhancing its transmission ability.
The alpha/B.1.1.7 SARS-CoV-2 variant exhibits significantly higher affinity for ACE-2 and requires lower inoculation doses to cause disease in K18-hACE2 mice.
Introduction: D614G mutants show a dramatically increased identified sample frequency (IF) and increases in fitness, infectivity and fatality.
Introduction: Thus, previous research has focused on adaptive SARS-CoV-2 mutants in S, such as D614G
Method: In accordance with previous findings, we also identified LG_1 (D614G), LG_2 (L84S) and LG_3 (R203K/G204R).
Method: There is no linkage disequilibrium between D614G and R203K/G204R (rho2 = 0.043, Table S1B), suggesting the independence in evolution between R203K/G204R and D614G.
Relative Consolidation of the Kappa Variant Pre-Dates the Massive Second Wave of COVID-19 in India.
Result: Except for the D614G mutation in the Spike protein, which is the defining feature of the B.1 lineage, no mutation was found to be shared between kappa and alpha variants.
Discussion: Of the 14 non-synonymous mutations observed across the genome of all the strains belonging to the kappa variant, five are found in the spike protein (E484Q, L452R, P681R, D614G, Q1071H) and among them the first two lie in the receptor binding domain (RBD) and the third one is adjacent to the furin cleavage site (Figure 7).
SARS_CoV_2 mutation literature information.
PMID: 
2021
BMC medicine
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