Abstract: The immune response to IN delivery of this SC2-spike DNA vaccine transported on a modified gold-chitosan nanocarrier shows a strong and consistent surge in antibodies (IgG, IgA, and IgM) and effective neutralization of pseudoviruses expressing S proteins of different SC2 variants (Wuhan, beta, and D614G).
Conclusion: The antibody response results in high levels of IgG and IgA, which show a strong neutralizing effect against pseudoviruses expressing different spike variants of SC2 (Wuhan, D614G and beta mutant).
Method: QHD43416.1; with
Figure: The relative inhibition in infectivity was performed against lentiviral particles engineered with (e) S protein SC2-Wuhan, (f) SC2-beta mutant, and (g) SC2-D614G mutant variants.
Characterization of SARS-CoV-2 Variants N501Y.V1 and N501Y.V2 Spike on Viral Infectivity.
PMID: 34722330
2021
Frontiers in cellular and infection microbiology
Result: Cathepsins inhibitor E64d treatment absolutely inhibited the infection of WT, D614G, N501Y.V1 and N501Y.V2 RBD pseudoviruses into 293T-hACE2 cells ( Figure 5A and Supplementary Figures S4A, B ), indicating that CatB/L is the dominant proteases required for priming of SARS-CoV-2 S protein in TMPRSS2- cells.
Result: Compared with D614G, N501Y.V2 pseudovirus had significant difference in the infection efficiency of 293T-hACE2-TMPRSS2, Caco2 and Caco2-hACE2 cells.
Result: Compared with WT pseudovirus, the viral infectivities of D614G and HV69-70 deletion were increased in all three cells.
Result: Compared with WT, pseudovirirus bearing S protein with
UK B.1.1.7 (Alpha) variant exhibits increased respiratory replication and shedding in nonhuman primates.
Introduction: In our current study, the AGM intranasal model of SARS-CoV-2 infection was used to assess differences between a contemporary progenitor SARS-CoV-2 variant (D614G), which was circulating in the summer of 2020, and the B.1.1.7 (Alpha) VOC that emerged from the D614G variant in the UK in late 2020.
Introduction: These variants were selected for direct comparison as they represent the two dominant SARS-CoV-2 variants circulating worldwide in late 2020 (D614G) and early 2021 (B.1.1.7).
Discussion: A recent study examining SARS-CoV-2 variants in the rhesus macaque model showed no difference in viral replication nor disease between the D614G and B.1.1.7 variants.
Discussion: B.1.1.7 replicated at higher levels in the respiratory tract resulting in lesions that were both more numerous and
Association between prognostic factors and the outcomes of patients infected with SARS-CoV-2 harboring multiple spike protein mutations.
Abstract: Besides the D614G mutation, we found L5F (18.8%), V213A (18.8%), and S689R (8.3%).
Result: All viruses contained the D614G variant, except one isolate.
Result: Besides the D614G mutation, the most common mutation in the S protein was L5F (18.8%), V213A (18.8%), and S689R (8.3%) (Table 3).
Discussion: A large-scale analysis of the COVID-19 Genomics UK consortium demonstrated that although D614G mutation is associated with higher viral loads, it is not associated with clinical severity and fatality of COVID-19<
Genomic surveillance reveals the detection of SARS-CoV-2 delta, beta, and gamma VOCs during the third wave in Pakistan.
Discussion: This mutation along with D614G had been found to decrease the sensitivity of convalescent sera and thus more likely to evade immune responses.
Discussion: This mutation in combination with D614G had demonstrated increased infectivity and enhanced transmissibility.
Insights on the SARS-CoV-2 genome variability: the lesson learned in Brazil and its impacts on the future of pandemics.
Abstract: From 720 SARS-CoV-2 genome sequences, we found few sites under positive selection pressure, such as the D614G (98.5 %) in the spike, that has replaced the old variant; the V1167F in the spike (41 %), identified in the P.2 variant that emerged from Brazil during the period of analysis; and I292T (39 %) in the N protein.
Introduction: Among them, the variants P.1 and P.2, first isolated in Brazil poss
Discussion: Another point to take into consideration is that the substitution D614G in the Spike protein was identified in almost 100 % of Brazilian sequences, in both periods, and, according to Zhang and coworkers, it replaced the D614-carrying virus becoming the dominant circulating strain worldwide.
A non-ACE2 competing human single-domain antibody confers broad neutralization against SARS-CoV-2 and circulating variants.
PMID: 34732694
2021
Signal transduction and targeted therapy
Result: During the circulation of SARS-CoV-2, the notable mutation D614G became the first dominant variant and replaced the ancestral spike.
Result: In align with this, the binding affinity of n3113.1-Fc with D614G spike showed no difference with the wild-type spike (WT S) (5.5 nM for WT S and 5.3 nM for D614G S.
Result: N3113.1-Fc neutralized pseudoviruses loaded with a spike of WT-D614G and Alpha variant in indiscriminate potent (IC50 of 0.07 mug/ml and 0.12 mug/ml for WT-D614G and Alpha, respectively.
Result: We generated a D614G mutation into the prefusion-stabilized spike for cryo-EM structure determination.
Emerging Severe Acute Respiratory Syndrome Coronavirus 2 Mutation Hotspots Associated With Clinical Outcomes and Transmission.
Introduction: The D614G variant in the S glycoprotein was associated with increased transmissibility, infectivity, and viral loads but not with disease severity.
Result: D614G increased from 74.6% to 99.9%; T265I, Q57H, R203K, and G204R started to increase in March and gradually decreased in July (Figure 3D).
Discussion: D614G and S194L were found to be associated with severe outcomes with high frequency worldwide.
Discussion: The D614G variant, which was found to occur in the S protein, has been proved to enhance infection and transmission.
Discussion: The SNV frequency of
Analysis of 329,942 SARS-CoV-2 records retrieved from GISAID database.
PMID: 34735950
2021
Computers in biology and medicine
Discussion: D614G is considered to be more infectious than the ancestral form but not associated with increased disease severity.
Discussion: For example, D614G is often considered together with P323L.
Discussion: Some researchers suggest the inability of D614G to cause viral success when presented alone.
Discussion: The most frequent mutation in the analyzed genes was a mutation in the S gene - A23403G (D614G), which was found in 94.15% of all studied genomes and in 99.9% of genomes uploaded in December 2020.
Discussion: The only interesting message was an article stating that this mutation co-mutates with infectivity-enhancing S protein mutations, such as
Discussion: After March 2020, the D614G clade became increasingly prevalent worldwide, expanding from 22 to 42 countries with GH (D614G + Q57H in the NS3) dominant in North America (59.0%).
Discussion: Our data from March showed that most of the SARS-CoV-2 had mutation particularly in D614G that may be D614G variant previously described.
Discussion: Our study found B.1.1.7 variant with mutation mainly in D614G, N501Y and del 69-70 was prevalent in all five months predominantly in April and May.
Discussion: The
Discussion: The emergence of new variants was led by the global rise of the D614G mutation in the Wuhan strain identified in China and Germany.
SARS_CoV_2 mutation literature information.
PMID: 
2021
ACS nano
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