Introduction: Delta variants have a number of characteristic mutations in their spike protein, including single-nucleotide polymorphisms (SNPs) resulting in T19R, R158G, L452R, T478 K, D614G, P681R, and D950N, which are usually chosen as the detection targets of the molecular diagnostics for Delta genotyping.
Analysis of SARS-CoV-2 variants B.1.617: host tropism, proteolytic activation, cell-cell fusion, and neutralization sensitivity.
Method: Horses were immunized using the SARS-CoV-2 RBD protein (original strain WH-1; RBD identical to the D614G reference strain) with Freund's incomplete adjuvant at an initial dose of 3 mg.
Method: Mice were immunized with purified SARS-CoV-2 plasmid comprising the D614G reference strain, B.1.351 variant, or B.1.429 variant (50 microg per mouse) at day 0.
Method: Nine samples were from patients in Beijing who had been infected with the D614G reference strain.
Figure: Data show the neutralization ID50 ratio of each variant, compared with the D614G reference strain.
Figure: Fluorescence signals of GFP were normalized to the signal of the D614G reference strain after 1 h of co-incubation; values shown indicate means +-
Fusogenicity and neutralization sensitivity of the SARS-CoV-2 Delta sublineage AY.4.2.
Result: As previously reported, the D614G and Alpha spike variants were less fusogenic than the Delta spike (Figure 2a, b, Figure S2).
Result: We previously reported using this assay that the spike protein of Alpha had the highest affinity to ACE2, followed by Delta and then by D614G.
Result: We thus analyzed the fusogenic activity of the AY.4.2 spike and compared it to the D614G, Alpha and Delta spikes.
Figure: Left Panel: Fusion was quantified by using the total GFP area/number of nuclei before normalizing to D614G for each experiment.
Figure: Statistical analysis: One-way ANOVA, each strain is compared to D614G or delta.
Design of SARS-CoV-2 Variant-Specific PCR Assays Considering Regional and Temporal Characteristics.
PMID: 35285246
2022
Applied and environmental microbiology
Result: As a result, for the Alpha variant, we identified nine mutations in the spike gene: S:Delta69/70, S:Delta144, S:N510Y, S:A570D, S:D614G, S:P681H, S:T716I, S:S982A, and S:D1118H.
Result: For the Delta variant, we identified seven mutations: S:T19R, S:Delta156/157, S<
SARS-CoV-2 infection after vaccination in Italian health care workers: a case report.
PMID: 35283546
2022
National Academy science letters. National Academy of Sciences, India
Abstract: Their genotyping performed on RNA extracts highlighted the presence of del69/70, N501Y, A570D, and 1841A > G (D614G) sequence variants, all indicative of VOC 202012/01-lineage B.1.1.7, suggesting a common source of infection.
Result: The genotyping performed first by Real-time PCR and then confirmed by direct sequencing proved the presence of del69/70, N501Y, A570D, and 1841A > G (D614G) variants, indicative of VOC 202,012/01-lineage B.1.1.7, in all samples suggesting a common source of infection.
Figure: Sections from the electropherograms showing the 69/70, N501Y, A570D, and D614G (a.
Cross-Neutralizing Breadth and Longevity Against SARS-CoV-2 Variants After Infections.
Discussion: Further follow-up will be needed to confirm whether the specific Nab titer against D614G is maintained or not.
Discussion: The neutralizing titer against D614G significantly decreased in sera of 6-8 months post onset compared to those of 1-3 months post onset ( Figure 3B ).
Discussion: The purpose of this study was to examine the longevity of Nab activity of COVID-19 convalescent sera against D614G, and their neutralizing breadth against B.1.1.7, P.1, and B.1.351.
Discussion: These observations suggested that all participants in this study were likely to be infected with D614G.
Discussion: This reason might be that weak immune-response against SARS-CoV-2 lead to the low Nab titers of 'patients without pneumonia' against PMID: 35273217
2022
Scientific reports
Method: Pre-fusion spike protein ectodomain DNA constructs were designed containing the following mutations compared to the Wuhan variant (Wuhan Hu-1; GenBank: MN908947.3): deletion of H69, V70 and Y144, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H in Alpha; L18F, D80A, D215G, L242H, R246I, K417N, E484K, N501Y, D614G and A701V in Beta;
Evolutionary history of the SARS-CoV-2 Gamma variant of concern (P.1): a perfect storm.
PMID: 35266951
2022
Genetics and molecular biology
Introduction: Currently, the WHO has identified the Gamma lineage (B.1.1.28.1, P.1 or Gamma; Nextstrain clade 20J/V3) with the following key S mutations: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, and V1176F.
Introduction: Key S mutations: L452R, D614G, P681R, +- (E484Q,
Table: D614G
A screening strategy for identifying the dominant variant of SARS-COV-2 in the fifth peak of Kurdistan- Iran population using HRM and Probe-based RT-PCR assay.
PMID: 35271889
2022
Journal of virological methods
Abstract: We screened the mutations of del69-70, E484K, E484Q, D614G, L452R, and T478K in 100 cases from SARS-COV-2 positive patients in Kurdistan- Iran population.
Introduction: D614G as a common mutation identified in most variants of SARS CoV-2 is occurred in the nucleotide sequence number 23403, leading to the change of amino acid codon from aspartate to glycine codon.
Result: 94 cases were detected in the CY5.5 channel for the mutant type of D614G and L452R mutations.
Result: Sequencing results overlapped with the results of the Primer-Probe assay, 94 samples had the D614G and L452R mutations with 5 samples encompassing
SARS_CoV_2 mutation literature information.
PMID: 
2022
Biomedicine & pharmacotherapy
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