Method: The S gene mutation plasmids (plasmid 1, containing the HV69-70del, K417N, E484K, N501Y, D614G, and P681H mutations, and plasmid 2, containing the L452R, E484Q, and P681R mutations) of SARS-CoV-2 variants (Alpha, Beta, Iota, Epsilon, Gamma, and Delta) were synthesized by Sangon Biotech (Shanghai, China).
Method: The S-F1/R1 amplification product contained one mutation type (HV69-70del), the S-F2/R2 amplification product contained five mutation types (K417N, E484K, E484Q, N501Y, and L452R), and
Re-emergence of Gamma-like-II and emergence of Gamma-S:E661D SARS-CoV-2 lineages in the south of Brazil after the 2021 outbreak.
Discussion: Although the results show an increase in infectivity close to 20-fold for the D614G/L452R protein, the increase in infectivity for D614G/W152C was fourfold when compared to the S protein containing only the D614G mutation.
Discussion: Among the main mutations common between the two groups, we highlight those that occur in the S protein: S:E484K, S:N501Y and S:D614G.
Discussion: For example, the D614G substitution enhanced viral stability and infectivity, and the E484 mutation in the re
On the association between SARS-COV-2 variants and COVID-19 mortality during the second wave of the pandemic in Europe.
PMID: 34790342
2021
Journal of market access & health policy
Introduction: Besides newly identified mutations, it cannot be ignored that SARS-COV-2 variants developed during the first wave, such as 20A (mutation S:D614G), developed in February 2020 and dominant during the previous wave, can also have impact on the mortality during the second wave.
Result: A significant negative correlation between the average proportion of 20A (S:D614G) variant and second-wave deaths peak height was observed for the period from start of the se
Table: D614G
Discussion: The proportion of 20A (mutation S:D614G) variant, firstly observed in Europe during the 1st wave of the pandemic (February 2020), was found to be negatively associated with the deaths peak height considering the pre-peak phase of the second wave.
Conformational dynamics and allosteric modulation of the SARS-CoV-2 spike.
Abstract: Here, using single-molecule Forster resonance energy transfer (smFRET) imaging we report the effects of ACE2 and antibody binding on the conformational dynamics of S from the Wuhan-1 strain and the B.1 variant (D614G).
Abstract: We find that D614G modulates the energetics of the RBD position in a manner similar to ACE2 binding.
SARS-CoV-2 exposure in Malawian blood donors: an analysis of seroprevalence and variant dynamics between January 2020 and July 2021.
Abstract: In a subset, we also assessed in vitro neutralisation against the original variant (D614G WT) and the Beta variant.
Method: SARS-CoV-2-pseudotyped lentiviruses were prepared by co-transfecting the HEK 293T cell line with either the SARS-CoV-2 original spike (D614G) or the SARS-CoV-2 beta spike (L18F, D80A, D215G, K417N, E484K
Result: Following previous observations that antibodies elicited by the original variant (D614G WT) are less potent against the beta variant, we reasoned that differential neutralisation potency by SARS-CoV-2 antibodies could be used to estimate the predominance of specific variants driving seroprevalence.
SARS-CoV-2 Delta (B.1.617.2) Variant: A Unique T478K Mutation in Receptor Binding Motif (RBM) of Spike Gene.
Introduction: SARS-CoV-2 delta variant shares three common mutation sites, L452R, D614G, and P681R with SARS-CoV-2 kappa and B.1.617.3 variant (Table 1).
Introduction: The D614G mutation site exists all four SARS-CoV-2 variants of concern as well as all six SARS-CoV-2 variants of interest and alert.
Table: D614G
Figure: The common D614G in all ten variant (Table 1) was indicated by bold blue letter.
Introduction and rapid dissemination of SARS-CoV-2 Gamma Variant of Concern in Venezuela.
PMID: 34800714
2021
Infection, genetics and evolution
Table: D614G
Implication of SARS-CoV-2 Immune Escape Spike Variants on Secondary and Vaccine Breakthrough Infections.
Abstract: This article highlights the pressures that facilitate the rise of new SARS-CoV-2 variants and the key mutations of the viral spike protein - L452R, E484K, N501Y and D614G- that promote immune escape mechanism and warrant a cautionary point for clinical and public health responses in terms of re-infection, vaccine breakthrough infection and therapeutic values.
Introduction: D614G mutation is located outside of the RBD (Figure 1).
Introduction: D614G variant bypassed natural bottlenecks due to its selective advantages that lead to improved viral infectivity and transmissibility and reduced viral sensitivity to neutralising convalescent ser
Table: D614G
Mutation-Induced Long-Range Allosteric Interactions in the Spike Protein Determine the Infectivity of SARS-CoV-2 Emerging Variants.
Result: Along with the VOC-/VOI-specific mutations, we further examined the effects of different flexible RBD orientations (i.e., clockwise and anticlockwise movements of RBD) that can be caused by several mutations at hinge residues located close to the S1/S2 junction (e.g., D614G).
Table: D614G
A rigorous framework for detecting SARS-CoV-2 spike protein mutational ensemble from genomic and structural features.
PMID: 34806033
2021
Current research in structural biology
Result: Followed by the RBD, the SD1 (542-591 a.a.) and SD2 (592-681 a.a.) regions showed a similar trend in both the WT and D614G systems with an average RMSD of 0.61 +- 0.003 nm.
Result: In early pandemic, L5F substitution in the signal peptide was present, alongwith D614G mutation in the linker.
Result: In the D614G trajectory, it corresponded to a relatively compact state with significantly less distance between the NTD and RBD (5.97 nm and 5.82 nm, respectively) as shown in.
SARS_CoV_2 mutation literature information.
PMID: 
2021
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