Abstract: Majority of sequenced strains (N = 60, 52%) were from the European cluster consistent with the higher infectivity rates associated with the D614G mutation carried by most strains in this cluster.

---

Result: Some mutations were more frequent than others; for example, the 241C>T, 3037C>T, 14408C>T, and the 23403A>G (also known as the D614G mutation in the S protein), were each seen in 58 of the genomes (Table S3).

---

Result: The predominance of European cluster is consistent with the higher infectivity rates associated with the D614G mutation carried by most strains in this cluster (Figure S1).

---

Introduction: A recent announcement by MOH revealed that five clusters in Malaysia, namely Benteng (23 viruses), Sivagangga (4 viruses), Tawar (3 viruses), Sungai (1 virus) and Bukit Tiram (1 virus); were found to display the D614G mutation in Spike protein.

---

Introduction: Analysis of more than 28,000 S gene sequence in May 2020 revealed that the variant carrying the D614G Spike mutation became the globally dominant form of SARS-CoV-2.

---

Introduction: Hence D614G genotype is likely to be more infectious, due to higher viral loads in COVID-19 patients infected with G614 variant.

---

Result: D614G, the most prevalent mutation globally, was also the most prevalent spike mutation in viral isolates from Iran, while showing an increasing trend from mid-May, observed mostly within the [B.1.*/20A] cluster.

---

Result: Moreover, Sanger sequencing of additional 67 SARS-CoV2 positive samples confirmed the increase in D614G frequency till October, becoming the dominant mutation in the Iranian outbreak (Figure 5).

---

Result: Remarkably, co-occurrence of D614G mutation with B.4 [G1397A-T28688C-G29742T] and G11083T variants was also observed in three samples.

---

Introduction: D614G/Q613H have now become established in circulating B/A lineage viruses.

---

Introduction: Both lineages have undergone significant diversification as they expanded; with this expansion apparently linked to a key S gene mutation D614G in lineage B and Q613H in lineage A:an apparent example of convergent evolution that resulted in a more stabilized spike protein, with D614G linked to modest increase in virus transmissibility.

---

Discussion: These variants appear to have expanded following the prior introduction of stabilizing mutations into the spike protein, such as D614G and Q613H.

---