SARS-CoV-2 mutations acquired during serial passage in human cell lines are consistent with several of those found in recent natural SARS-CoV-2 variants.
PMID: 35474907
2022
Computational and structural biotechnology journal
Introduction: For example, the D614G and P681R mutations in the spike protein were associated with increased viral replication in human lung epithelial cells and enhanced viral pathogenicity, respectively.
Discussion: Following the noticeable increase in the D614G mutation in April 2020, the Omicron variant has acquired over 30 mutations, including those found in the Alpha, Beta, Gamma, and Delta variants.
Discussion: However, in this experiment, key natural mutations such as D614G were not observed, which could be due to the specific host cells used or the in vitro culture system.
Safety and Immunogenicity of Inactivated COVID-19 Vaccines Among People Living with HIV in China.
Discussion: However, the GMT for the delta variant was significantly lower than the GMT for the D614G variant in PLWH, which is consistent with Chang Liu's finding that mRNA vaccines induced an antibody response to some variants, but the neutralization of the delta variant was reduced.
Discussion: SARS-CoV-2 IgG concentrations and neutralizing antibody titers against the D614G and delta variants also declined significantly in PLWH compared to HDs.
Discussion: The D614G and delta variants harbor mutations in the RBD, so neutralizing titers to the pseudotyped virus may better show the immune response to the two variants elicited by vaccination than titers of S-RBD-IgG.
Discussion: The positive rates of neut
Biomechanical Dependence of SARS-CoV-2 Infections.
Abstract: Given the recent data highlighting the importance of alternative virulent strains, we included both the native strain identified in early 2020 and an early S protein variant (D614G) that was shown to increase the viral infectivity markedly.
Abstract: Our results show that cells on softer and sparser scaffolds, closer resembling younger lungs, exhibit higher infection rates by the WT and D614G variant.
RBD-mRNA vaccine induces broadly neutralizing antibodies against Omicron and multiple other variants and protects mice from SARS-CoV-2 challenge.
Abstract: The vaccine induced durable antibodies that potently neutralized prototypic strain and B.1.1.7 lineage variant pseudoviruses containing N501Y or D614G mutations alone or in combination with a N439K mutation (B.1.258 lineage), with a L452R mutation (B.1.427 or B.1.429 lineage), or a L452R-E484Q double mutation (B.1.617.1 variant), although neutralizing activity against B.1.1.7 lineage variant containing 10 amino acid changes in the S protein was slightly reduced.
The twin-beginnings of COVID-19 in Asia and Europe-one prevails quickly.
Abstract: The first wave is a group of four mutations (C241T, C3037T, C14408T and A23403G [this being the amino acid change D614G]; all designated 0 to 1 below).
Abstract: This DG (D614G) group, fixed at the start of the pandemic, is the foundation of all subsequent waves of strains.
Whole genome sequencing of SARS-CoV2 strains circulating in Iran during five waves of pandemic.
Abstract: There were different mutations in all parts of the genomes but Spike-D614G, NSP12-P323L, N-R203K and N-G204R were the most frequent mutants in these studied viruses.
Delta variant (B.1.617.2) of SARS-CoV-2: Mutations, impact, challenges and possible solutions.
PMID: 35507895
2022
Human vaccines & immunotherapeutics
Abstract: The enhanced transmissibility of Delta variant has been associated with critical mutations such as D614G, L452R, P681R, and T478K in the S-protein.
COVID-19 outbreak in Malaysia: Decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang.
Introduction: The objective of our study was to determine in CoronaVac-vaccinated people in Chile, with a complete two-dose schedule, the levels of neutralizing antibodies against D614G, alpha, and gamma variants.
Method: Neutralization assays were done es
Figure: GMTs and 95% CIs are indicated: D614G (75.1), alpha (18.5), gamma (10.05) variant.
Figure: Neutralization assays with D614G (lineage B, blue circles), alpha (lineage B.1.1.7, red circles) and gamma (lineage P.1, green circles) variants were done.
Figure: Neutralization assays with infectious D614G (lineage B), alpha (lineage B.1.1.7) and gamma (lineage P.1) variants were done.
Figure: Patterns of neutralizing antibody titers of 44 CoronaVac-vaccinated human sera against infectious D614G, alpha and gamma SARS-CoV-2 variants.
Assessment of the binding interactions of SARS-CoV-2 spike glycoprotein variants.
PMID: 34545316
2022
Journal of pharmaceutical analysis
Method: SARS-CoV-2 (2019-nCoV) spike S1 rabbit monoclonal antibody (mAb1), SARS-CoV-2 (2019-nCoV) spike neutralizing rabbit monoclonal antibody (mAb2), and recombinant proteins corresponding to the original SARS-CoV-2 spike RBD; original S1; spike S1 variant D614G; and RBD protein variants N501Y, N439K, Y453F, and E84K were purchased from Sino Biological (Wayne, PA, USA).
Result: Such finding is consistent with that of recent studies where the increased infectivity of viruses with the D614G mutation was not found to be explained by a greater ACE2 binding activity.
Analyzing the effect of mutations in SARS-CoV2 papain-like protease from Saudi isolates on protein structure and drug-protein binding: Molecular modelling and dynamics studies.
PMID: 34548835
2022
Saudi journal of biological sciences
Introduction: Some of these mutations have relatively high incidences, such as a non-synonymous mutation (D614G) in the virus's S protein.
Discussion: Furthermore, D614G, due to the higher flexibility, gains an increase in its thermostability as compared to the WT virus.
Discussion: This is of substantial importance because earlier reports show that spike protein substitution to glycine in D614G mutation increases the flexibility due to the shorter side chain allowing a more efficient cleavage of S protein subunits, which might explain the improved ACE binding affinity.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Computational and structural biotechnology journal
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