literature

SARS_CoV_2 mutation literature information.

Evolutionary history of the SARS-CoV-2 Gamma variant of concern (P.1): a perfect storm.

PMID: 35266951 2022 Genetics and molecular biology

Introduction: Currently, the WHO has identified the Gamma lineage (B.1.1.28.1, P.1 or Gamma; Nextstrain clade 20J/V3) with the following key S mutations: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, and V1176F.

Introduction: Key S mutations: L452R, D614G, P681R, +- (E484Q, Q107H,

A screening strategy for identifying the dominant variant of SARS-COV-2 in the fifth peak of Kurdistan- Iran population using HRM and Probe-based RT-PCR assay.

PMID: 35271889 2022 Journal of virological methods

Result: 94 cases were detected in the CY5.5 channel for the mutant type of D614G and L452R mutations.

Result: Sequencing results overlapped with the results of the Primer-Probe assay, 94 samples had the D614G and L452R mutations with 5 samples encompassing Del69/70.

Result: The 5 samples had detected by the Primer-Probe UK variant had the same melting curves that differed from 89 cases containing D614G, L452R, and T478K mutations.

A SARS-CoV-2 Wuhan spike virosome vaccine induces superior neutralization breadth compared to one using the Beta spike.

PMID: 35273217 2022 Scientific reports

Abstract: In addition, neutralizing activity against the D614G, Alpha and Delta variants was also significantly lower after Beta spike vaccination than after Wuhan spike vaccination.

Method: Pre-fusion spike protein ectodomain DNA constructs were designed containing the following mutations compared to the Wuhan variant (Wuhan Hu-1; GenBank: MN908947.3): deletion of H69, V70 and Y144, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H in Alpha; L18F, D80A, D215G, L242H,

COVID-19 outbreak in Malaysia: Decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang.

PMID: 35273335 2022 Communications biology

Introduction: Experimental data from human lung epithelium and animal models revealed that the D614G substitution increased virus infectivity and transmissibility as compared to an original D614 strain.

Introduction: From January 2020, viruses carrying the spike D614G mutation emerged in several countries.

Introduction: In June, D614G SARS-CoV-2 lineage B.1 became the dominant form of circulating virus worldwide and replaced the initial SARS-CoV-2 strains related to the outbreak in Wuhan, China.

SARS-CoV-2 Mutations and Their Impact on Diagnostics, Therapeutics and Vaccines.

PMID: 35273977 2022 Frontiers in medicine

Introduction: D614G in the RBM domain has shown to increase the S-protein density on the viral surface, thereby enhancing infectivity.

Introduction: And cilgavimab had lower activity against N501Y+D614G mutants, including B.1.429 (carrying L452R), B.1.617.2 (carrying L452R + T478K) and B.1.351 (carrying K417N + E484K + N501Y).

Introduction: Both P4715L and P323L was observed along with S protein D614G mutation, suggesting a co-evolutionary pattern.

Potential inhibitor for blocking binding between ACE2 and SARS-CoV-2 spike protein with mutations.

PMID: 35279013 2022 Biomedicine & pharmacotherapy

Discussion: Beta variant contains nine mutations in SARS-CoV-2 spike protein: D614G, Delta242-Delta244, R246I, K417N, E484K, N501Y, and A701V.

Cross-Neutralizing Breadth and Longevity Against SARS-CoV-2 Variants After Infections.

PMID: 35281007 2022 Frontiers in immunology

Discussion: Further follow-up will be needed to confirm whether the specific Nab titer against D614G is maintained or not.

Discussion: The neutralizing titer against D614G significantly decreased in sera of 6-8 months post onset compared to those of 1-3 months post onset ( Figure 3B ).

Discussion: The purpose of this study was to examine the longevity of Nab activity of COVID-19 convalescent sera against D614G, and their neutralizing breadth against B.1.1.7, P.1, and B.1.351.

Discussion: These observations suggested that all participants in this study were likely to be infected with D614G.

Discussion: This reason might be that weak immune-response against SARS-CoV-2 lead to the low Nab titers of 'patients without pneumonia' against

PMID: 35283546 2022 National Academy science letters. National Academy of Sciences, India

Abstract: Their genotyping performed on RNA extracts highlighted the presence of del69/70, N501Y, A570D, and 1841A > G (D614G) sequence variants, all indicative of VOC 202012/01-lineage B.1.1.7, suggesting a common source of infection.

Result: The genotyping performed first by Real-time PCR and then confirmed by direct sequencing proved the presence of del69/70, N501Y, A570D, and 1841A > G (D614G) variants, indicative of VOC 202,012/01-lineage B.

Figure: Sections from the electropherograms showing the 69/70, N501Y, A570D, and D614G (a., b., c., d.) associated with SARS-CoV-2 Alfa Variant B.1.1.7.

Design of SARS-CoV-2 Variant-Specific PCR Assays Considering Regional and Temporal Characteristics.

PMID: 35285246 2022 Applied and environmental microbiology

Result: As a result, for the Alpha variant, we identified nine mutations in the spike gene: S:Delta69/70, S:Delta144, S:N510Y, S:A570D, S:D614G, S:P681H, S:T716I, S:S982A, and S:D1118H.

Result: For the Delta variant, we identified seven mutations: S:T19R, S:Delta156/157, S<

Fusogenicity and neutralization sensitivity of the SARS-CoV-2 Delta sublineage AY.4.2.

PMID: 35290827 2022 EBioMedicine

Introduction: The spike protein of Delta contains 9 mutations, when co

Method: The D614G spike plasmid was generated by introducing the mutation into the Wuhan reference strain via Q5 site-directed mutagenesis (NEB).

Result: As previously reported, the D614G and Alpha spike variants were less fusogenic than the Delta spike (Figure 2a, b, Figure S2).

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