Introduction: The spike protein D614G mutation, for example, has been implicated in more effective transmission, although the actual impact may be through fixation in an expanding lineage rather than conferring increased transmissibility per se.
Method: Identification of S-gene D614G mutation in Basel sequences.
Method: Viral load (Ct-value) of patients that carried lineages with a mutated S-D614G gene (N = 274) were compared to patients that carried the ancestral allele (N = 12) using a Mann-Whitney U test.
Result: Among our samples, we found no significant difference in viral loads between patients with and without the S-D614G mutation (z = -0.881, p = 0.38).
Result: The
Multiple SARS-CoV-2 variants escape neutralization by vaccine-induced humoral immunity.
Figure: (A and B) Pseudovirus neutralization (pNT50) is plotted for all individuals that received one dose (bottom panels) or two full doses (upper panels) of either the BNT162b2 (A) or mRNA-1273 (B) vaccines for each of the following SARS-CoV-2 pseudoviruses: wild-type, D614G, B.1.1.7, B.1.1.298, B.1.429, P.2, P.1, and three variants of the B.1.351 lineage denoted as B.1.351 v1, v2, and v3.
Figure: (B) Representative pseudovirus neutralization curves are shown for an individual >=7 days out from the second dose of BNT162b2 vaccine comparing wild-type SARS-CoV-2 pseudovirus to the following variant pseudoviruses: D614G (pink); B.1.1.7 (purple); B.1.1.298 (blue); B.1.429 (green); P.2 (yellow); P.1 (orange); B.1.351 v1, v2, and v3 (red); SARS-CoV (brown); and WIV1-CoV (black).
Figure: (B) World map depicting the locations where the variants of these lineages we
Global Geographic and Temporal Analysis of SARS-CoV-2 Haplotypes Normalized by COVID-19 Cases During the Pandemic.
Result: How these effects occur at the structural level remains unclear, although some hypotheses have been put forward: (1) We think that there is no evidence for hydrogen-bond between D614 and T859 mentioned by, and distances between D614 and T859 are too long for a hydrogen bond (Supplementary Figure S13B), (2) distances between Q613 and T859 (Supplementary Figure S13C) could be reduced by increased flexibility due to D614G substitution, forming a stabilizing hydrogen bond, and (3) currently available structures do not show salt-bridges between D614 and R646 as proposed by; Supplementary Figure S13D).
Result: It has been suggested that the D614G change in the S1 domain that results from the A23403G mutation gener
Result: analyzed the entropy of variation of these two mutations (D614G and P323L) until May.
SARS-CoV-2 Genome from the Khyber Pakhtunkhwa Province of Pakistan.
Result: The 20A/G clades are characterized by D614G mutation in spike protein, suggesting increased transmissibility but not pathogenicity.
Result: The most common mutations detected in the Pakistani isolates were NSP6_L37F (5), spike_D614G, NSP12_P323L (4), and NS3_Q57H (4).
Result: The variant D614G in S protein detected in the current study is more commonly present in European isolates, such as those from Spain, Belgium, France, Italy, Switzerland, and the Netherlands, and appears more severe and fatal, accounting for a huge death toll (); Germany, Kuwait, an
COVID-19 outbreak in Malaysia: Decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang.
PMID: 33750399
2021
Theoretical biology & medical modelling
Conclusion: Our findings show a link between the molecular-level mutation activity of SARS-CoV-2 and population-level COVID-19 transmission to provide further evidence for a positive association between the D614G substitution and Rt.
Result: Hence, the changing dynamics of Rt are likely associated with the key mutations that are solely contributed to by the D614G substitution.
Result: However, we noticed that since (roughly) April 15, 2020, the prevalence of the D614G substitution increased, but Rt remained constant.
Result: In other words, the D614G substitution is considered a key mutation and is likely dominant in accounting for the changes in COVID-19 transmissibility due to a mutation at the molecular level.
Result: Previous analysis implie
Higher infectivity of the SARS-CoV-2 new variants is associated with K417N/T, E484K, and N501Y mutants: An insight from structural data.
PMID: 33755190
2021
Journal of cellular physiology
Introduction: Novel mutations in the spike protein of B.1.1.7 (deletion 69-70, 144 and substitution K417N, K417T, E484K, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H, and many others) might have altered the SARS-CoV-2 ability to transmit and infect.
Result: Comparative genomics inspection of genomes obtained from the UK, South Africa, Brazil, and other parts of the world revealed that the spike protein had acquired multiple crucial mutations including (K417N, K417T, E48
Whole-genome sequencing of SARS-CoV-2 reveals the detection of G614 variant in Pakistan.
Result: According to GISAID clade nomenclature (https://www.gisaid.org/references/statements-clarifications/clade-and-lineage-nomenclature-aids-in-genomic-epidemiology-of-active-hcov-19-viruses/), Pakistani strains NIH-44905 and NIH-HAS001 belong to S clade having characteristic genetic markers C8782T, T28144C, and NS8-L84S whereas strains NIH-45090, NIH-45143, and NIH-45579 belong to GH clade having marker variations C241T, C3037T, A23403G, G25563T, S-D614G, and NS3-Q57H.
Result: Five missense mutations were common in all the strains, three in
Pan-India novel coronavirus SARS-CoV-2 genomics and global diversity analysis in spike protein.
Abstract: The study suggests that SARS-CoV-2 genomes from India share consensus with global trends with respect to D614G as most prevalent mutational event (81.66% among 2525 Indian isolates).
Result: Among all the variations, twelve (L5F, L8V, L18F, R21I
Discussion: It was noted that D614G mutant strains in India increased rapidly during eight months of the pandemic and is present in more than 81% of the genome sequences reported from India.
Discussion: The recent studies by Korber et al and WHO collaborating study in China demonstrated D614G strains are 10-fold more infectious than the original Wuhan-1 strain.
Discussion: Yet even in such states, the association with D614G could not be found.
The N501Y spike substitution enhances SARS-CoV-2 transmission.
Introduction: The first dominant mutation observed in SARS-CoV-2 encoded the spike protein D614G substitution, which enhances viral replication in human airway epithelial cells and viral transmission in animal models.
Result: These mutations were engineered into the USA-WA1/2020 strain containing the D614G spike substitution that has become dominant worldwide due to its increased transmission efficiency; the resulting SARS-CoV-2 strain [USA-WA1/2020-G614, hereafter called wild-type (wt)], was generated using site-directed mutagenesis of a cDNA clone.
Figure: a, The reverse genetic construction design of all the individual and combined mutations on the wt D614G backbone.
Figure: b, The location of all 8 UK B.1.1.7 substitutions and D614G on the SARS-CoV-2
Chimeric spike mRNA vaccines protect against Sarbecovirus challenge in mice.
Abstract: Chimeric spike mRNA vaccines efficiently neutralized D614G, UK B.1.1.7., mink cluster five, and the South African B.1.351 variant of concern.
Method: Full-length SARS-CoV-2 Seattle, SARS-CoV-2 D614G, SARS-CoV-2 B.1.351, SARS-CoV, WIV-1, and RsSHC014 viruses were designed to express nanoluciferase (nLuc) and were recovered via reverse genetics as described previously.
Result: 2C) and SARS-CoV-2 NTD (Fig 2D), mice immunized with chimeras 1-4 in the prime and boost generated similar magnitude binding antibodies to SARS-CoV-2 D614G compared to mice immunized with the
Discussion: Consistent with previous studies that measured efficacy of mRNA-LNP vaccines for SARS-CoV-2 vaccines in mice, our monovalent SARS-CoV-2 vaccine elicited robust neutralizing antibody titers to the SARS-CoV-2 D614G variant.
SARS_CoV_2 mutation literature information.
PMID: 
2021
PLoS pathogens
Browser Board
Co-occurred Entities
Filtrator
ViMRT