Abstract: Our results indicated that the mean neutralization ED50 of these sera against Omicron decreased to 66, which is about 8.4-folds compared to the D614G reference strain (ED50 = 556), whereas the neutralization activity of other VOC and VOI pseudotyped viruses decreased only about 1.2-4.5-folds.
Introduction: The neutralization sensitivity of PV-Omicron against serum samples from a panel of COVID-19 convalescent patients was tested, using PV-D614G (S protein from SARS-CoV-2 D614G strain) as the reference.
Result: The ED50 of Alpha, Beta, and Gamma is reduced about 1.2, 2.8, and 1.6-fold, respectively, compared to the reference strain PV-
Figure: The neutralization ED50 and ratio compared to the reference strain D614G was also displayed as indicated.
Rapid and accurate detection of SARS-CoV-2 mutations using a Cas12a-based sensing platform.
Result: D614G detection is thus more suitable for samples when SARS-CoV-2 viral load is <10-14 M (approximately 6000 copies/muL), and appropriate dilution of samples should be considered for higher viral loads (Table S5).
Result: All samples carried the D614G mutation.
Result: Because CRISPR/Cas12a has been reported to differentiate SNPs with single-base resolution, we used Cas12a to identify SARS-CoV-2 N501Y, D614G, and 69/70 deletion mutations; these are the key mutations of SARS-CoV-2 VOC (Table S1).
Result: However, 40 min is the most advisable for D614G detection.
Result: However, significant differences in fluorescence intensities between 614-crRNA-W and 614-crRNA-M were noted, and this slight activation had no impact on D614G discrimination.
A comprehensive overview of identified mutations in SARS CoV-2 spike glycoprotein among Iranian patients.
Introduction: They reported that S mutation D614G increases infectivity.
Result: The shared characteristic feature of these three clades is S_D614G mutation, which may increase the infectivity of SARS-CoV-2.
Result: There were also three sequences carrying D138Y+ S477N+ D614G triple mutations sampled from shiraz and Tehran.
Table: D614G
Discussion: Also, there were two other sequences sampled from Shiraz in January 2021, which had five specific mutations as indicator of the Alpha variant (D614G, H69del, N501Y, V70del, Y144del) and were cluste
Emergence of a novel SARS-CoV-2 Pango lineage B.1.1.526 in West Bengal, India.
PMID: 34896696
2022
Journal of infection and public health
Discussion: D614G, the first mutation appeared in the S glycoprotein, was detected in Germany in January 2020 and became the dominant mutation in all the circulating strains worldwide by June 2020.
Discussion: Based on non-synonymous mutational analysis, here we revealed the emergence of a novel SARS-CoV-2 lineage B.1.1.526 harboring 11 coexisting mutations in seven different genes including D614G, P681H and V1230L in the S glycoprotein.
Discussion: Hamster infected with the D614G variant produces higher infectious virus titers in nasal washes and the trachea, supporting the role of D614G mutation in high transmissibility.
Discussion: However, D614G does not al
Efficacy of mRNA, adenoviral vector, and perfusion protein COVID-19 vaccines.
Introduction: A regular genome sequence is essential for viral specimens, particularly in the global context of a pandemic, since it aids to identify any emerging SARS-CoV-2 genetic strains, most importantly, with the development of the worldwide prevalence of D614G strain, which was linked with enhanced disease transmission however could not produce severe disease, the genetic transition was negligible at first.
Introduction: Consequently, ten mutations in the spike protein of B.1.617.2 strain, including T19R, (G142D*), 156/157del, R158G, L452R, T478K, D614G, P681R, and D950N were identified.
Introduction: In the PMID: 34921776
2022
Cell host & microbe
Method: The Beta virus used in these studies contained the following mutations: D80A, D215G, L242-244 deleted, K417N, E484K, N501Y, D614G, A701V.|mg
Result: However, Alpha also contains the mutation D614G, which is not found in Victoria/Wuhan, we therefore went back and tested neutralization of a version of the early pandemic virus (B.1) containing the additional D614G mutation, using 17 Alpha sera.
Figure: (G) FRNT50 titers of 17 Alpha convalescent sera against Alpha and B.1 (D614G), analysis used the Wilcoxon matched-pairs signed rank sum test, and two-tailed p values were calculated; geometric means are indicated above each column.
Pierce into Structural Changes of Interactions Between Mutated Spike Glycoproteins and ACE2 to Evaluate Its Potential Biological and Therapeutic Consequences.
PMID: 34931119
2022
International journal of peptide research and therapeutics
Result: According to the results of the multiple sequence alignment, the mutations of the EPI_ISL_601443 variant were as follows: H69 deletion, V70 deletion, Y144 deletion, N501Y substitution, A570D substitution, D614G substitution, P681H substitution, T716I substitution, S982A substitution, and D1118H substitution.
Mutation profile of SARS-CoV-2 genome in a sample from the first year of the pandemic in Colombia.
PMID: 34933126
2022
Infection, genetics and evolution
Abstract: In the latter, we identified mutations E484K, L18F, and D614G.
Result: The mutations p.Lys1191Asp (K1191D), p.Glu484Lys (E484K), p.Leu18Phe (L18F), p.Pro26Ser (P26S), p.His655Tyr (H655Y), p.Asp614Gly (D614G), that along with other nucleotide substitutions constitute some of the VOC an
Enhanced fitness of SARS-CoV-2 variant of concern Alpha but not Beta.
Introduction: Beyond S(D614G), Alpha has 18 further mutations in its genome compared with the progenitor, with two deletions and six substitutions within the S gene.
Introduction: Here we investigate the fitness of Alpha and Beta VOCs relative to wt-S614G, the predominant parental strain containing the S(D614G) substitution:in relevant primary airway culture systems in vitro, and in ferrets, Syrian hamsters and two mouse models expressing human ACE2:to assess specific advantages in replication and transmission and to evaluate the effects of Alpha S mutations alone in vivo.
Introduction: In March 2020, a single amino acid change in the S protein at position 614 (S( PMID: 34939673
2022
Journal of medical virology
Abstract: Noteworthily, the receptor binding domain (RBD) region of S protein of all the isolates harbored similar substitutions including K417N, E484K, and N501Y that signify the Beta, while D614G, D215G, D80A, A67V, L18F, and A701V substitutions were commonly found in the non-RBD region of Spike proteins.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Emerging microbes & infections
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