Method: The mutant D614G SARS-CoV-2 Spike gene was obtained from Addgene (158075).
Result: D614G, the common mutation of the SARS-CoV-2 Spike protein in alpha, beta, gamma, and delta SARS-CoV-2 variants, has been reported to enhance virus replication and transmission.
Result: DMPC and PMPC also potently inhibited infection of SARS-CoV-2 pseudovirus harboring Spike D614G mutation in HEK293T-ACE2 and Vero E6 cells.
Result: Importantly, trimyristin treatment also inhibited D614G mutant SARS-CoV-2 infection.
Discussion: Importantly, DMPC and PMPC inhibit both wild-type and D614G mutant pseudotyped SARS-CoV-2 infe
Interaction Analysis of the Spike Protein of Delta and Omicron Variants of SARS-CoV-2 with hACE2 and Eight Monoclonal Antibodies Using the Fragment Molecular Orbital Method.
PMID: 35312321
2022
Journal of chemical information and modeling
Introduction: The S protein mutations in the alpha variant are DeltaH69/DeltaV70, Delta144/144, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H.
Introduction: The mutations of the S protein in the Delta variant are T19R, E156G, Delta157/158, L452R, T478K, D614G, P681R, and D950N.
The SARS-CoV-2 Delta variant induces an antibody response largely focused on class 1 and 2 antibody epitopes.
Introduction: We compare the specificity of Delta-elicited antibodies to those elicited by earlier SARS-CoV-2 variants, including the early 2020 (i.e., Wuhan-Hu-1 and D614G) and Beta variants.
Result: As expected, Delta breakthrough infection resulted in higher neutralizing titers against both D614G (by ~4.8-fold) and Delta (by ~3.8-fold) spikes than 2x BNT162b2 alone.
Result: As expected, the 2x BNT162b2 and Delta breakthrough plasmas most potently neutralized the D614G spike, and primary Delta infection plasmas most potently neutralized the Delta spike (Fig 6 and S7).
Result: Compared to the Wuhan-Hu-1 prototypical early 2020 virus, Delta has multiple mutations in the spike protein: T19R, Delt
SARS-CoV-2 Omicron variant: Immune escape and vaccine development.
Introduction: Previous studies illustrated that D614G reduces the binding affinity to ACE2 but enhances the protease cleavage of S1/S2, leading to higher transmissibility.
Introduction: Specifically, BA.1 and BA.2 display 20 identical spike mutations, which are G339D, S373P, S375F, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P68
Conformational dynamics and allosteric modulation of the SARS-CoV-2 spike.
Introduction: At the same time, the enhanced exposure of the RBM in the D614G variant led to increased sensitivity to neutralizing antibodies.
Introduction: By the summer of 2020, the SARS-CoV-2 S variant D614G (B.1 lineage) had supplanted the ancestral virus (strain Wuhan-1) worldwide, and structural analysis showed that D614G disrupts an interprotomer contact.
Introduction: Nonetheless, antibodies that target the S2 stalk further promoted the RBD-up conformation on the D614G spike.
Introduction: The D614G spike existed in an equilibrium where the RBD favors the up conformation prior to antibody binding.
Coronavirus Genomes and Unique Mutations in Structural and Non-Structural Proteins in Pakistani SARS-CoV-2 Delta Variants during the Fourth Wave of the Pandemic.
Result: The most common non-synonymous and indel mutations, present in all complete genome samples were spike_T478K, spike_T19R, spike_L452R, spike_F157del, spike_E156G, spike_P681R, spike_D614G, spike_R158del, and spike_G142D (Table 3).
Neutralisation Hierarchy of SARS-CoV-2 Variants of Concern Using Standardised, Quantitative Neutralisation Assays Reveals a Correlation With Disease Severity; Towards Deciphering Protective Antibody Thresholds.
Introduction: As the pandemic progressed, a number of single amino acid mutations in the Spike protein were detected, such as D614G and A222V.
Introduction: Referred to as Cluster 5 or B.1.1.298, several different groups of mutations were identified, with the most abundant population containing missense and deletion mutations on the Spike; 69/70del, Y453F and D614G.
Introduction: The D614G mutation was found to increase the density of Spike protein on virions and infectivity.
Table: D614G
Functional analysis of polymorphisms at the S1/S2 site of SARS-CoV-2 spike protein.
3Introduction: The D614G exchange increases the percentage of S proteins present in the ""open"" conformation required for efficient ACE2 binding and viruses bearing this exchange show accelerated transmission kinetics in animal models."
Introduction: Nevertheless, mutations in SARS-CoV-2 have been detected and viruses with a D614G exchange became dominant early in the pandemic.
Discussion: Why mutant S686G showed enhanced interaction with ACE2 is at present unclear but might be due to its RBD adopting a conformation that may favour ACE2 binding, which would be a similar effect as reported for mutation D614G that is also located outside of the RBD.
Circulation of SARS-CoV-2 Variants among Children from November 2020 to January 2022 in Trieste (Italy).
Result: Briefly, in all sequences (32/32, 100%), the D614G substitution was detected, lineage B.1.
Result: Finally, 1/32 sequence was the Omicron strain, with the following mutations in the RBD domain: T22882G (N440K), G22898A (G446S), G22992A (S477N), C22995A (T478K), A23013C (E484A), A23040G (Q493R), G23048A (G496S), A23055G (Q498R),
Delta Variant with P681R Critical Mutation Revealed by Ultra-Large Atomic-Scale Ab Initio Simulation: Implications for the Fundamentals of Biomolecular Interactions.
3Introduction: In addition to the P681R mutation, the Delta variant also contains a D614G mutation, which promotes the RBD of the S-protein in an ""open"" conformation, making its binding with the ACE2 receptor easier, as well as enhancing the protease cleavage at the S1/
Abstract: Here, using ultra large-scale ab initio computational modeling, we study the P681R and D614G mutations in the SD2-FP domain, including the effect of double mutation, and compare the results with the wild type.
Abstract: Together with the earlier reported mutation D614G in the same domain, it offers an excellent instance to investigate the nature of mutations and how they affect the interatomic interactions in the spike protein.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Metabolism
Browser Board
Co-occurred Entities
Filtrator
ViMRT