Result: After the post-infection treatment, curcumin exerted an antiviral effect against SARS-CoV-2 D614G strain at 10 microg/mL of 84.4%, p = 0.0095, and at 5 microg/mL of 31.7%, p = 0.0095 (Figure 5).
Result: As can be seen from Figure 6, the viral titer of D614G strain was significantly reduced through co-treatment strategy (incubation of curcumin with the virus prior to infection) at 10 (92%, p = 0.004), 5 (60.4%, p = 0.004), and 2.5 microg/mL (39.3%, p = 0.004) of curcumin (Figure 6).
Result: By pre-post infection treatment (cells treatment with curcumin, prior and post to infection with SARS-CoV-2) curcumin exerted antiviral activity of 99.0% (p = 0.0095), 51.3% (p = 0.0095), 22.2% (p = 0.0095), and 27.8% (p = 0.0095) against SARS-CoV-2 D614G strain at concentrations of 10, 5, 2.5, and 1.25 microg/mL, respectively (Figure 3).
Result: Curcumin Inhibited SARS-CoV-2
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021.
Result: The D614G and P323L occurred in China on 24 January 2020 and in the U.K.
Result: The B.1 and its sub-lineages that carry both D614G in spike and P323L in nsp12 substitutions have become the dominant variants across the world.
Result: We found amino acid substitutions in Spike (D614G), N (D377Y, P67S, P199L), NS3 (G172V, Q57H), NS8 (S24L) NSP2 (T85I
High Individual Heterogeneity of Neutralizing Activities against the Original Strain and Nine Different Variants of SARS-CoV-2.
Method: Eleven patients were infected by spike D614G-harbouring B lineage strains that spread during the first wave of COVID-19 infections in France, 9 were infected by the Marseille-4/B.1.160 variant, 10 were infected by the Alpha variant, and 12 were infected by the Beta/B.1.351.2 variant.
"Macrophages and Monocytes: ""Trojan Horses"" in COVID-19."
Abstract: We aimed to explore whether variants of SARS-CoV-2 (Chinese-derived strain (D614, lineage A), Italian strain PV10734 (D614G, lineage B.1.1) and Alpha strain (lineage B.1.1.7)) were able to infect monocytes (MN) and monocyte-derived macrophages (MDM) and whether these infected cells may, in turn, be vectors of infection.
Method: SARS-CoV-2 strains, including the wild type Chinese-derived strain (D614, lineage A), Italian strain PV10734 (D614G, lineage B.1.1) and Alpha variant (lineage B.1.1.7), were isolated at 33 C from infected patients' nasal swabs in the permissive VERO E6 (VERO C1008 (Vero 76, clone E6, Vero E6; ATCC1CRL-1586TM) cell line.
The Evolutionary Landscape of SARS-CoV-2 Variant B.1.1.519 and Its Clinical Impact in Mexico City.
Discussion: Importantly, they also inferred that the Pro323Leu mutation occurred on an Asp614Gly background.
Discussion: They identified that the Pro323Leu mutation in the RNA-dependent RNA polymerase led to the rapid spread of the virus, rather than the previously reported Asp614Gly mutation in the spike glycoprotein.
Antibody Titer Kinetics and SARS-CoV-2 Infections Six Months after Administration with the BNT162b2 Vaccine.
Abstract: Neutralization activity against the D614G, B.1.1.7, and B.1.351 variants were also analyzed.
Method: Three clinical isolates of SARS-CoV-2 were obtained and propagated in Vero E6 cells as previously described: D614G (hCoV-19/Italy/UniSR1/2020; GISAID Accession ID: EPI_ISL_413489), B.1.1.7 (Alpha) (19/Italy/LOM-UniSR7/2021; GSAID Accession ID: EPI_ISL_1924880), B.1.351 (Beta) (hCoV-19/Italy/LOM-UniSR6/2021, GISAID Accession ID: EPI_ISL_1599180).
Result: The highest AUC was observed for the D614G variant followed by the B.1.1.7 and the B.1.351 (Figure 4).
Result: We also evaluated the neutralizing activity at T2 and T3 of the 48 collected sera (diluted to 1:80 and 1:160) against three SARS-CoV-2 strains isolated in our laboratory and belonging to the variants D614G, B.1.1.7, and B.1.351 (hCoV-19/Italy/LOM-UniSR-1/2020; hCoV-19/I
Phylogenetic and full-length genome mutation analysis of SARS-CoV-2 in Indonesia prior to COVID-19 vaccination program in 2021.
PMID: 34840498
2021
Bulletin of the National Research Centre
Result: In this study, D614G mutation was detected in 103 Indonesian SARS-CoV-2 isolates.
Discussion: An antibody escape is considered as another mutation mechanism like the upcoming form of D614G which can be accelerated by an antigenic drift.
Discussion: Based on these recent studies, the virus virulence and the increase of viral loads in COVID-19 patients characterize the occurrence of D614G mutation (Korber et al.; Zhang et al.), while, based on the current available information, the infectivity as well as the receptor binding, fusion activation, or ADE enhancement can be influenced by D614G mutation in several ways (Ulrich et al.; Wang and Zand.; Nidom et al.).
Discussion: Besides, recent publications show that one of the most notable amino acid mutations is D61
Codon usage, phylogeny and binding energy estimation predict the evolution of SARS-CoV-2.
PMID: 34841034
2021
One health (Amsterdam, Netherlands)
Abstract: Second, the host-specific D614G mutation becomes prevalent starting from March 2020.
Introduction: Another amino acid variation of the spike p
Result: Notably, D614G is recorded for SARS-CoV and other human CoVs since 2004.
Result: The D614G mutation and its relationship with the reservoirs.
Result: The first noteworthy mutation that became prevalent as early as mid-2020 is D614G.
Figure: B) Diffusion of D614D/E/N/G substitution in bat, porcine and human coronavirus.
Discussion: The D614G is present in data referred to humans, for both SARS-CoV-2 and SARS-CoV.
Discussion: This confirms D614G as a good marker to track viral evolution.
A next generation sequencing (NGS) analysis to reveal genomic and proteomic mutation landscapes of SARS-CoV-2 in South Asia.
PMID: 34841355
2021
Current research in microbial sciences
Abstract: While analyzing transmission source in terms of geolocation, the largest clustered group from the South Asian countries was based on the G-clade (D614G) (81.7%; 335/410 samples), tracing the inception and transmission of SARS-CoV-2 infections in the South Asian countries from European regions.
Conclusion: In this study, through analyzing the patterns and localizations of both genomic and proteomic mutations in SARS-CoV-2 isolates fro
Result: In this case, we used one of the most common and well-studied D614G mutation found in the spike protein as a control agent of this analysis.
Result: The largest clustered group of the samples was found based on the G-clade (D614G), containing 81.7% (335/410 samples) of the total sample count.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Diagnostics (Basel, Switzerland)
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