5Result: As the control of this experiment, the pseudoviruses with the S protein of the D614G-bearing isolate (B.1 lineage), which spread in the early pandemic (i.e., during early 2020 and before the emergence of VOCs/VOIs), were prepared, and this is referred to as ""parental virus"" in this study."
Method: Plasmids expressing the S proteins of the Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Delta (B.1.617.2), and Lambda (C.37) variants and the point mutants were generated by site-directed overlap extension PCR using pC-SARS2-S D614G as the template and the following primers listed in Table S6.
Result: However, the lower infectivity of the Alpha variant compared to the D614G-bearing early pandemic virus, analyzed by pseudovirus assay (Figure 2A), is consistent with a pr
Comparative mutational analysis of SARS-CoV-2 isolates from Pakistan and structural-functional implications using computational modelling and simulation approaches.
PMID: 34968862
2022
Computers in biology and medicine
Conclusion: The data revealed that the D614G mutation particularly contributes to the enhanced infectivity.
Method: The spike mutants include N74K, D614G, V622F, Q677H, S943T, D115G with the human receptor ACE2 using the HAWKDOCK server.
Table: D614G
Figure: (A) Among the Structural proteins,
Figure: (a, b, c) Superimposed structure of wild-type spike protein (green) with D614G-V622F-S943F (red), and D614G-Q677H (orange) and.
Computational modelling of potentially emerging SARS-CoV-2 spike protein RBDs mutations with higher binding affinity towards ACE2: A structural modelling study.
PMID: 34979405
2022
Computers in biology and medicine
Introduction: These variants, such as B.1.17 (Alpha variant) harbouring a mutation N501Y reported in the UK, D614G variant, lineage B.1.1.207 with P681H replacement, lineage B.1.351 (Beta) with E484K mutation reported in South Africa (B.1.351) and P.1 variant reported in Brazil.
Genotype-phenotype correlation identified a novel SARS-CoV-2 variant possibly linked to severe disease.
PMID: 33506644
2022
Transboundary and emerging diseases
Abstract: Majority of sequenced strains (N = 60, 52%) were from the European cluster consistent with the higher infectivity rates associated with the D614G mutation carried by most strains in this cluster.
Result: Some mutations were more frequent than others; for example, the 241C>T, 3037C>T, 14408C>T, and the 23403A>G (also known as the D614G mutation in the S protein), were each seen in 58 of the genomes (Table S3).
Result: The predominance of European cluster is consistent with the higher infectivity rates associated with the D614G mutation carried by most strains in this cluster (Figure S1).
Discussion: Most of those strains (58/60) carried the D614G
COVID-19 outbreak in Malaysia: Decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang.
Introduction: A recent announcement by MOH revealed that five clusters in Malaysia, namely Benteng (23 viruses), Sivagangga (4 viruses), Tawar (3 viruses), Sungai (1 virus) and Bukit Tiram (1 virus); were found to display the D614G mutation in Spike protein.
Introduction: Analysis of more than 28,000 S gene sequence in May 2020 revealed that the variant carrying the D614G Spike mutation became the globally dominant form of SARS-CoV-2.
Introduction: Hence D614G genotype is likely to be more infectious, due to higher viral loads in COVID-19 patients infected with G614 variant.
Introduction: Nevertheless, it has been observed that the spike D614G subs
SARS-CoV-2 outbreak in Iran: The dynamics of the epidemic and evidence on two independent introductions.
PMID: 33835709
2022
Transboundary and emerging diseases
Discussion: Monitoring the spike mutations in Iranian SARS-CoV-2 genomes revealed no novel mutations in this genomic region, while determined two commonly known mutations, T22I and D614G.
Discussion: Notably, in accordance with the increasing [B.1*/20A] clade among the viral isolates of the Iranian epidemic as of mid-May, the D614G variant is dominating in this population.
Discussion: The increase in frequency of D614G mutation and B.1* lineages from mid-May onwards predicts a rapid viral transmission followed by considerable change in the composition of viral lineages circulating in the country.
Discussion: Variation tracking of SARS-CoV-2 has shown that some mutations:such as P323L and D614G:are distributed globally, while some ot
Spike protein cleavage-activation mediated by the SARS-CoV-2 P681R mutation: a case-study from its first appearance in variant of interest (VOI) A.23.1 identified in Uganda.
Introduction: D614G/Q613H have now become established in circulating B/A lineage viruses.
Introduction: Both lineages have undergone significant diversification as they expanded; with this expansion apparently linked to a key S gene mutation D614G in lineage B and Q613H in lineage A:an apparent example of convergent evolution that resulted in a more stabilized spike protein, with D614G linked to modest increase in virus transmissibility.
Discussion: These variants appear to have expanded following the prior introduction of stabilizing mutations into the spike protein, such as D614G and Q613H.
Molecular definition of severe acute respiratory syndrome coronavirus 2 receptor-binding domain mutations: Receptor affinity versus neutralization of receptor interaction.
Discussion: Earlier research proposed an association of the S:D614G with mortality; however, this might be difficult to interpret owing to this substitution's early global dominance.
Discussion: The spike amino acid changes that were most common after S: D614G included N501Y and P681H, with a notable increase in diversity compared with March 2020.
Discussion: This lineage shows amino acid substitutions NSP12:P323L, N:S194L,S:D614G, and S:P681H in >90% of
SARS-CoV-2 Genome from the Khyber Pakhtunkhwa Province of Pakistan.
Result: The 20A/G clades are characterized by D614G mutation in spike protein, suggesting increased transmissibility but not pathogenicity.
Result: The most common mutations detected in the Pakistani isolates were NSP6_L37F (5), spike_D614G, NSP12_P323L (4), and NS3_Q57H (4).
Result: The variant D614G in S protein detected in the current study is more commonly present in European isolates, such as those from Spain, Belgium, France, Italy, Switzerland, and the Netherlands, and appears more severe and fatal, accounting for a huge death toll (); Germany, Kuwait, an
SARS_CoV_2 mutation literature information.
PMID: 
2022
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