Method: Horses were immunized using the SARS-CoV-2 RBD protein (original strain WH-1; RBD identical to the D614G reference strain) with Freund's incomplete adjuvant at an initial dose of 3 mg.
Method: Mice were immunized with purified SARS-CoV-2 plasmid comprising the D614G reference strain, B.1.351 variant, or B.1.429 variant (50 microg per mouse) at day 0.
Method: Nine samples were from patients in Beijing who had been infected with the D614G reference strain.
Figure: Data show the neutralization ID50 ratio of each variant, compared with the D614G reference strain.
Figure: Fluorescence signals of GFP were normalized to the signal of the D614G reference strain after 1 h of co-incubation; values shown indicate means +-
RT-LAMP assay for rapid detection of the R203M mutation in SARS-CoV-2 Delta variant.
Introduction: Delta variants have a number of characteristic mutations in their spike protein, including single-nucleotide polymorphisms (SNPs) resulting in T19R, R158G, L452R, T478 K, D614G, P681R, and D950N, which are usually chosen as the detection targets of the molecular diagnostics for Delta genotyping.
The SARS-CoV-2 Delta variant induces an antibody response largely focused on class 1 and 2 antibody epitopes.
Introduction: We compare the specificity of Delta-elicited antibodies to those elicited by earlier SARS-CoV-2 variants, including the early 2020 (i.e., Wuhan-Hu-1 and D614G) and Beta variants.
Result: As expected, Delta breakthrough infection resulted in higher neutralizing titers against both D614G (by ~4.8-fold) and Delta (by ~3.8-fold) spikes than 2x BNT162b2 alone.
Result: As expected, the 2x BNT162b2 and Delta breakthrough plasmas most potently neutralized the D614G spike, and primary Delta infection plasmas most potently neutralized the Delta spike (Fig 6 and S7).
Result: Compared to the Wuhan-Hu-1 prototypical early 2020 virus, Delta has multiple mutations in the spike protein: T19R, Delt
2'-O-Methyl modified guide RNA promotes the single nucleotide polymorphism (SNP) discrimination ability of CRISPR-Cas12a systems.
Conclusion: Secondly, HBV genotyping and SARS-CoV-2 D614G mutant biosensing platforms were established to validate the high specificity and versatility of the Cas12a system.
Result: 2'-OMe modifications of gRNA increased the specificity for the SARS-CoV-2 D614G mutant detection.
Result: Accordingly, we designed one unmodified gRNA complementary to the SARS-CoV-2 D614G mutant (ugRNA-D614G).
Result: For target DNA at 1 nM and 0.1 nM, the final DF values of mgRNA-D614G-3' (1.43 +- 0.08 and 1.8 +- 0.1 for 1 nM and 0.1 nM, respectively) were abo
Result: all unmodified and modified gRNAs-D614G possessed high specificity even in the complex sample, demonstrating the stability of the Cas12a based D614G mutant biosensing platform.
Reduced DMPC and PMPC in lung surfactant promote SARS-CoV-2 infection in obesity.
Abstract: DMPC and PMPC markedly inhibited wild type and D614G mutant SARS-CoV-2 infection in HEK293T-ACE2 and Vero-E6 cells.
Abstract: Lipid extract from BALF of trimyristin-treated obese mice mitigated the elevated wild type and D614G mutant SARS-CoV-2 infection.
Result: D614G, the common mutation of the SARS-CoV-2 Spike protein in alpha, beta, gamma, and delta SARS-CoV-2 variants, has been reported to enhance virus replication and transmission.
Result: DMPC and PMPC also potently inhibited infection of SARS-CoV-2 pseudovirus harboring Spike D614G mutation in HEK293T-ACE2 and Vero E6 cells.
Result: Importantly, trimyristin treatment also inhibited
Interaction Analysis of the Spike Protein of Delta and Omicron Variants of SARS-CoV-2 with hACE2 and Eight Monoclonal Antibodies Using the Fragment Molecular Orbital Method.
PMID: 35312321
2022
Journal of chemical information and modeling
Introduction: The S protein mutations in the alpha variant are DeltaH69/DeltaV70, Delta144/144, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H.
Introduction: The mutations of the S protein in the Delta variant are T19R, E156G, Delta157/158, L452R, T478K, D614G, P681R, and D950N.
Clinico-Genomic Analysis Reiterates Mild Symptoms Post-vaccination Breakthrough: Should We Focus on Low-Frequency Mutations?
Introduction: One such VOC, B.1.617.2, also known as the Delta variant and first identified in India in October 2020, is characterized by mutations T19R, G142D, Delta157-158, R158G, L452, T478K, D614G, P681R, and D950N in the spike protein.
Table: D614G
SARS-CoV-2 Omicron variant: Immune escape and vaccine development.
Introduction: Previous studies illustrated that D614G reduces the binding affinity to ACE2 but enhances the protease cleavage of S1/S2, leading to higher transmissibility.
Introduction: Specifically, BA.1 and BA.2 display 20 identical spike mutations, which are G339D, S373P, S375F, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P68
Conformational dynamics and allosteric modulation of the SARS-CoV-2 spike.
Introduction: At the same time, the enhanced exposure of the RBM in the D614G variant led to increased sensitivity to neutralizing antibodies.
Introduction: By the summer of 2020, the SARS-CoV-2 S variant D614G (B.1 lineage) had supplanted the ancestral virus (strain Wuhan-1) worldwide, and structural analysis showed that D614G disrupts an interprotomer contact.
Introduction: Nonetheless, antibodies that target the S2 stalk further promoted the RBD-up conformation on the D614G spike.
Introduction: The D614G spike existed in an equilibrium where the RBD favors the up conformation prior to antibody binding.
SARS_CoV_2 mutation literature information.
PMID: 
2022
EBioMedicine
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