literature

SARS_CoV_2 mutation literature information.

High throughput sequencing based direct detection of SARS-CoV-2 fragments in wastewater of Pune, West India.

PMID: 34688738 2022 The Science of the total environment

Result: Notably, nine mutations in the Spike region (S: L452R, S:C480R, S: E484Q, S: D614G, S: P681R, S: N801, S: D950N, S: Q1071H, S: P1140) were observed in this study (Table 1, Supplementary Table 2).

Table: D614G

Anti-Severe Acute Respiratory Syndrome Coronavirus 2 Hyperimmune Immunoglobulin Demonstrates Potent Neutralization and Antibody-Dependent Cellular Cytotoxicity and Phagocytosis Through N and S Proteins.

PMID: 34693968 2022 The Journal of infectious diseases

Abstract: METHODS: Functional characterization of anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) hyperimmune immunoglobulin (hIG) from human convalescent plasma was performed by different virus neutralization methodologies (plaque reduction, virus-induced cytotoxicity, median tissue culture infectious dose [TCID50] reduction, and immunofluorimetry) at different laboratories using geographically different SARS-CoV-2 isolates (USA [1], Italy [1], and Spain [2]; 2 containing the D614G mutation).

Abstract: Neutralization capacity against the original Wuhan SARS-CoV-2 strain and variants (D614G mutant, B.1.1.7, P.1, and B.1.351) was evaluated using a pseudovirus expressing the corresponding spike (S) protein.

A broadly cross-reactive antibody neutralizes and protects against sarbecovirus challenge in mice.

PMID: 34726473 2022 Science translational medicine

Abstract: Here, we demonstrate the neutralization of SARS-CoV; bat coronaviruses WIV-1 and RsSHC014; and SARS-CoV-2 variants D614G, B.1.1.7, B.1.351, P.1, B.1.429, B.1.526, B.1.617.1, and B.1.617.2 by a receptor binding domain (RBD)-specific human antibody, DH1047.

Distinct shifts in site-specific glycosylation pattern of SARS-CoV-2 spike proteins associated with arising mutations in the D614G and Alpha variants.

PMID: 34735575 2022 Glycobiology

Abstract: Despite maintaining an overall similar structural conformation, our mass spectrometry-based site-specific glycosylation analyses of similarly produced spike proteins with and without the D614G and Alpha variant mutations reveal a significant shift in the processing state of N-glycans on one specific NTD site.

Abstract: The Alpha variant derived from the D614G lineage is distinguished from others by having deletion mutations located right within an immunogenic supersite of the spike N-terminal domain (NTD) that make it refractory to most neutralizing antibodies directed against this domain.

Introduction: The D614G mutation in the S protein was among the first identified in the early phase of the

Identification of genotypic variants and its proteomic mutations of Brazilian SARS-CoV-2 isolates.

PMID: 34740719 2022 Virus research

Table: D614G

Discussion: Certain studies reported the increment in viral load in the upper respiratory tract (URT) of patients infected with the D614G mutant type virus.

Discussion: Several studies have implied that the D614G mutation in the spike glycoprotein of SARS-CoV-2, being closely located in the receptor-binding domain.

Analysis of SARS-COV2 spike protein variants among Iraqi isolates.

PMID: 34754982 2022 Gene reports

Abstract: The most frequently mutations occurred at the D614G (87/91), followed by S982A (50/91), and A570D (48/91), respectively.

Abstract: Twenty-two distinct mutations were identified within the spike protein regions which were: L5F, L18F, T19R, S151T, G181A, A222V, A348S, L452 (Q or M),

Introduction: A variant that carries D614G mutation in the spike protein of the virus, also known as G clade or B.1, was diagnosed during the early days of the pandemic in the north of America, and then reported in many European countries.

Reduced levels of convalescent neutralizing antibodies against SARS-CoV-2 B.1+L249S+E484K lineage.

PMID: 34780883 2022 Virus research

Introduction: In February 2020, in the early stages of the pandemic, the B.1 lineage emerged in Europe with the characteristic D614G mutation in the S protein, which distinguishes it from the A and B ancestral lineages.

Result: Regarding B.1.111 isolates, although both share the mutation pattern characteristic of the B.1.111 lineage (Spike D614G, NS3 Q57H and NSP12 P323L), several divergent genome wide mutations were observed between those isolates, for example, EPI_ISL_794659 have two additional mutations at the S protein (Spike T859I

Table: D614G

Longitudinal analysis of SARS-CoV-2 spike and RNA-dependent RNA polymerase protein sequences reveals the emergence and geographic distribution of diverse mutations.

PMID: 34801754 2022 Infection, genetics and evolution

Result: D614G has been shown to alter the conformational state of the receptor binding domain through a hinge mechanism involving its loop structure, and it is possible that A222V may have a similar effect on the conformational state.

Result: D614G quickly became the dominant variant on all continents, though its rate of establishment was much lower in Asia.

Result: D614G reached 90% prevalence in Asia during the week of June 14

Analysis of Glycosylation and Disulfide Bonding of Wild-Type SARS-CoV-2 Spike Glycoprotein.

PMID: 34817202 2022 Journal of virology

Result: Both the S1 and S2 glycoproteins of the wild-type and D614G SARS-CoV-2 strains migrated faster when expressed in the GALE/GALK2 293T cells compared with the migration of these glycoproteins expressed in 293T cells.

Result: Both the wild-type SARS-CoV-2 S glycoprotein and the prevalent D614G variant S glycoprotein were expressed in 293T cells and in the GALE/GALK2 293T cells.

Figure: (A) The wild-type SARS-CoV-2 S glycoprotein (D614, with an aspartic acid residue at 614) and the D614G variant (G614, with a glycine residue at 614) were expressed in wild-type 293T cells (wt) or in GALE/GALK2 293T cells (ko).

Reduced sensitivity of the SARS-CoV-2 Lambda variant to monoclonal antibodies and neutralizing antibodies induced by infection and vaccination.

PMID: 34818119 2022 Emerging microbes & infections

Fig

Figure: (C) Analysis of the ID50 values of neutralization reactions between the D614G reference and Lambda pseudoviruses to convalescent sera.

Figure: Analysis of the ID50 values of the neutralization reactions for vaccine-immunized sera between D614G and Lambda pseudoviruses (E, F, G, H).

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