Discussion: Also, D614G variant is found to be more sensitive than the WT in presence of two-pore channel (TPC) blocker tetrandrine (Figure S4).
Discussion: In summary, the enhanced infectivity of the Delta spike compared to Alpha, Beta, and D614G viruses largely results from the increased affinity of the spike protein to Ca2.
Discussion: Our study demonstrated that SARS-CoV-2 spike is a dynamic calcium sensor, and D614G mutation and evolved spike strains B.1.1.7, B.1.351, and B.1.617.2 elicit enhanced dynamic calcium sensitivity leading to fast and efficient membrane fusion for entry.
Discussion: Remarkably, the WT spike, D614G, and evolved spike variants a
The basis of mink susceptibility to SARS-CoV-2 infection.
Introduction: However, there is no data available that any of the mutations, which were expected to be crucial for
Figure: Abbreviations stand for: CT, cytoplasmic domain; D614G, mutation in the S protein; F486L, mutation in the S protein; FP, fusion peptide; HR1, heptapeptide repeat sequence 1; HR2, heptapeptide repeat sequence 2; N501T, mutation in the S protein; NDT, N-terminal domain; PRRA, polybasic cleavage site; RBD, receptor-binding domain; S1, S1 subunit of the S protein; S2, S2 subunit of the S protein; TM, transmembrane domain; Y453F, mutation in the S protein.
Emergence and phenotypic characterization of the global SARS-CoV-2 C.1.2 lineage.
Method: A subset of eleven of these samples were used to test neutralization activity against Delta (N = 11) and C.1.2 (N = 11) using the pseudovirus neutralization assay and ADCC (N = 9) activity against D614G and C.1.2.
Method: ChAdOx1 nCOV-19 (AZD1222) Vaccinees: samples from donors vaccinated with the ChAdOx1 nCOV-19 (AZD1222) vaccine were previously assessed for neutralization activity against the D614G (N = 11) and Beta variants (N = 11).
Method: HEK293T cells were transfected with 5 mug of SARS-CoV-2 wild-type variant spike (D614G), Beta, Delta or C.1.2 spike plasmids using PEI-MAX 40,000 (Polysciences) and incubated for 2 days at 37C.
Method: Janssen/Johnson and Johnson (Ad26.COV2.S) Vaccinees: samples from healthy donors vaccinated with the Janssen/Johnson and
A self-amplifying RNA vaccine protects against SARS-CoV-2 (D614G) and Alpha variant of concern (B.1.1.7) in a transmission-challenge hamster model.
Result: All SARS-CoV-2 S saRNA-vaccinated hamsters showed serum neutralizing antibody levels against D614G virus similar to or higher than those in two human convalescent sera collected more than 3 weeks after recovery from first wave infection whereas there was no S specific IgG nor neutralizing activity detected in pre immunization sera (not shown) or in sera from influenza HA vaccinated hamsters.
Result: For a subset of hamsters (7/12), there was sufficient serum volume to re-test the neutralizing activity against the Alpha VOC, and titres against this variant were not significantly different than against the D614G virus (Fig S1a).
Result: Six hamsters received 103 PFU of a D614G isolate from UK collected in summer 2020, B.1.238, and six received 103 P
Table: D614G
Phylogeography and genomic epidemiology of SARS-CoV-2 in Italy and Europe with newly characterized Italian genomes between February-June 2020.
Abstract: In conclusion, within the limitations of phylogeographical reconstruction, the estimated ancestral scenario suggests an important role of China and Italy in the w
Result: Seventeen amino acid substitutions were present in more than 10% of the Italian isolates but only one of them was in the spike protein (D614G).
Result: the analyses showed that B.1 probably originated in China and spread to several European countries reaching Italy several times, forming a large cluster which included initially 59 (around the first week of March) and finally 198 genomes, and 6 further independent introductions mainly corresponding to a group of genomes characterized only by the substitution D614G but lacking other substitutions, in particular the P314L in the RdRp identifying the clade 20A (lineage B.1, clade 19A).
Multiple SARS-CoV-2 Variants Exhibit Variable Target Cell Infectivity and Ability to Evade Antibody Neutralization.
Discussion: However, in Caco2-hACE2, 293T-hACE2, and 293
Discussion: LY-CoV555 has immune escape against almost all variants except D614G and B.1.17.
Discussion: Similar to the neutralizing activity of antibodies, LY-CoV555 had reduced binding capacity for almost all variants except D614G and B.1.17.
Discussion: Studies show that the D614G substitution leads to the enhancement of viral replication and infectivity in host cells.
Discussion: The present study shows that, compared with the D614G strain, the infectivity of most variants was changed in mammalian cells.
Discussion: showed that the fusion activity among D614G, B.1.1.7, B.1.351, P.1, B.1.617.1, and B.1.617.2 had no significant differences when spike and ACE2 were transfected at high levels.
Atorvastatin Effectively Inhibits Ancestral and Two Emerging Variants of SARS-CoV-2 in vitro.
Discussion: Consistent with this report, our data showed that ATV also inhibited D614G strain in Caco-2 (EC50 = 7.4 muM), a colorect
Discussion: In the present study, the antiviral effect of atorvastatin against SARS-CoV-2 D614G strain was identified by the treatment of Vero E6 cells at different times of infection.
Discussion: In this study, Vero E6 cells were treated with ATV for 48 h after infection, obtaining a reduction of the infectious SARS-CoV-2 D614G particles at all evaluated concentrations.
Discussion: Similar to this report, our study demonstrated that ATV inhibited D614G strain at 31.2 muM by pretreating cells for 1 h.
Discussion: The results presented in this article also showed a reduction of the intracellular D614G strain RNA after post-treatment with ATV at 31.2 and 15.6 muM.
Impact of B.1.617 and RBD SARS-CoV-2 variants on vaccine efficacy: An in-silico approach.
PMID: 35370005
2022
Indian journal of medical microbiology
Discussion: D614G, T20N, D138Y, L18F, R190S, and P26S in the NTD and K417T, E484K and N501Y in the RBD region and H655Y within the furin cleava
Discussion: The mRNA-1273 vaccine's neutralizing activity towards number of variants like B.1.351, B.1.1.7 + E484K, B.1.1.7, P.1, B.1.427/B.1.429, D614G, 20A.EU2, 20E [EU1], N439K-D614G, and previously identified mutant in Denmark mink cluster 5 were identified and found to have the same neutrality level as Wuhan-Hu-1 (D1414).
Rapid SARS-CoV-2 Intra-Host and Within-Household Emergence of Novel Haplotypes.
Introduction: The first mutation known to improve viral fitness was Spike D614G, which was first identified in the Alpha variant and then reached a prevalence of nearly 100% globally.
Landscape-Based Protein Stability Analysis and Network Modeling of Multiple Conformational States of the SARS-CoV-2 Spike D614G Mutant: Conformational Plasticity and Frustration-Induced Allostery as Energetic Drivers of Highly Transmissible Spike Variants.
PMID: 35377633
2022
Journal of chemical information and modeling
Abstract: In this study, we employed efficient and accurate coarse-grained simulations of multiple structural substates of the D614G spike trimers together with the ensemble-based mutational frustration analysis to characterize the dynamics signatures of the conformational landscapes.
Abstract: The recent structural and biophysical studies provided important evidence about multiple conformational substates of the D614G spike protein.
Abstract: The results suggest that the D614G mutant may employ a hinge-shift mechanism in which the dynamic couplings between the site of mutation and the interprotomer hinge modulate the interdomain interactions, global mobility change, and the increased stability of the open form.
Abstract: The structural and functional studies of the SARS-CoV-2
SARS_CoV_2 mutation literature information.
PMID: 
2022
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