Inhibitor screening using microarray identifies the high capacity of neutralizing antibodies to Spike variants in SARS-CoV-2 infection and vaccination.
3Introduction: For example, the D614G variant, which was first identified in July 2020, has a faster infection rate and higher viral load in the upper respiratory tract than the wild-type ""Wuhan-Hu-1"" strain."
Abstract: In addition, similar results were obtained using a SARS-CoV-2 pseudovirus neutralization assay specific for wild-type S and five prevalent S variants (D614G, B.1.1.7, B.1.351, P.1, B.1.617.2), thus demonstrating that high antibody diversity is associated with high NAb titers.
Introduction: The B.1.1.7 variant (D614G, N501Y) is more infectious and may lead to increased mortality compared to the parental strain.
Result: Consistent with the observation that the trimerized form of the D614G PMID: 35403837
2022
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Abstract: Pseudotyped and authentic virus-based assays show that COVID-HIG displays broad-spectrum neutralization effects on a wide variety of SARS-CoV-2 variants, including D614G, Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Kappa (B.1.617.1), Delta (B.1.617.2), and Omicron (B.1.1.529) in vitro.
Pathogenicity of SARS-CoV-2 Omicron (R346K) variant in Syrian hamsters and its cross-neutralization with different variants of concern.
Method: On deep sequencing following amino acid changes were found in the isolate.(NSP5_P132H,Spike_T95I,Spike_K417N,Spike_S373P,Spike_Q493R,Spike_N969K, Spike_H655Y,Spike_N856K,N_R203K,Spike_S371L,NSP3_A1892T, PMID: 35419205
2022
Evolution, medicine, and public health
Conclusion: Assuming that the D614G genotype is better adapted than its ancestor in Vero cells, this observation is consistent with the hypothesis that fitter genotypes adapt at a slower pace.
Conclusion: Here, we followed the evolution in cells of two strains of SARS-CoV-2: one with the original spike protein (CoV-2-D) and one carrying the D614G mutation (CoV-2-G), a variant that emerged in the early phase of the pandemic and soon became prevalent.
Conclusion: The two genomic backgrounds shared several de novo mutations, suggesting that the D614G mutation on the spike protein did not
Result: This D614G mutation in the spike protein emerged early in the pandemic, increased the infectivity of the virus and became prevalent worldwide.
Efficacy of vaccination and previous infection against the Omicron BA.1 variant in Syrian hamsters.
Introduction: Here, in the hamster model, we addressed these issues under controlled conditions using the Omicron variant and isolates that are no longer circulating in nature (i.e., a Wuhan-like isolate and an isolate with only a D614G spike mutation).
Split T7 promoter-based isothermal transcription amplification for one-step fluorescence detection of SARS-CoV-2 and emerging variants.
Abstract: Moreover, we provide experimental evidence of the broad applicability of this assay through the multiplex detection of SARS-CoV-2 variants (D614G mutation) and direct detection of bacterial 16S rRNA.
Introduction: D614G), conferring greater infectivity and more rapid spread, have become predominant in various regions.
Introduction: Moreover, STAR was utilized for multiplex detection of the D614G mutation and N gene of SARS-CoV-2 in a single tube as well as for the direct detection of bacterial 16S rRNA without additional nucleic acid purification, thereby confirming the wide applicability of this method for nucleic acid biomarker detection.
Figure: (A) Illustration of the STAR assay for the D614G mutation in the S gene of SARS-CoV-2.
Figure: (C) Lim
Comparative genomics, evolutionary epidemiology, and RBD-hACE2 receptor binding pattern in B.1.1.7 (Alpha) and B.1.617.2 (Delta) related to their pandemic response in UK and India.
PMID: 35427787
2022
Infection, genetics and evolution
Result: Again, like the previous point AA mutation, we also evaluated the D614G mutation, which was identified as the B.1.1.7 variant.
Result: In the D614G structure, the amino acid changed from Asp614 Gly.
Result: The structural evaluation of the D614G mutation was performed, showing different forms of interactions.
Result: The structural landscape of mutation analysis revealed significant mutations, such as N501Y, D614G L452R, E484Q, and P681R, which are frequently found in these two variants.
Result: The structural landscape of significant mutations (N501Y, D614G
Prolonged shedding of infectious viruses with haplotype switches of SARS-CoV-2 in an immunocompromised patient.
PMID: 35430092
2022
Journal of infection and chemotherapy
Conclusion: When haplotype 1 was compared with Wuhan-Hu-1/2019 (GenBank accession number; MN908947), it had ten non-synonymous (ORF1a:Q2702H and S2981F, ORF1b:P314L, T1404 M, P1567L, and R2684I, S gene:D614G, N gene:R203K, G204R, and M234I) and five synonymous mutations.
Role of the Microbiome in the Pathogenesis of COVID-19.
PMID: 35433495
2022
Frontiers in cellular and infection microbiology
Abstract: Soon after the first outbreak due to the wild-type strain in December 2019, a genetic variant D614G emerged in late January to early February 2020 and became the dominant genotype worldwide.
Conclusion: The two most commonly occurring mutations, Spike_D614G and Nsp12_P314L, were structurally modeled, which showed that these mutations had the potential to enhance viral entry and replication, respectively.
Introduction: Among these, the D614G clade was the most common and was first found in late January 2020 in China, according to a study conducted by.
Introduction: Earliest samples from the USA appeared to have been derived from China and belonged to basal or L84S
Table: D614G
Antigenicity comparison of SARS-CoV-2 Omicron sublineages with other variants contained multiple mutations in RBD.
Result: D614G original virus can be well neutralized by D614G, VOCs (Alpha, Beta, Gamma, Delta), and VOIs (Lambda, Mu) spike protein immunized sera, with NT50 values of 12,130, 9238, 4871, 3535,
Result: Among them, the D614G immunized serum had the strongest neutralization protection to the original D614G strain.
Result: Besides, the sera immunized with spike protein from D614G, Alpha, Delta, and Mu variants are more protective against D614G, B.1.640.1, and B.1.630 variants.
Result: Cluster analysis showed that D614G, B.1.640.1, and B.1.630 showed similar antigenicity to different immunogens (Figure 3C).
SARS_CoV_2 mutation literature information.
PMID: 
2022
Theranostics
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