Abstract: Moreover, we provide experimental evidence of the broad applicability of this assay through the multiplex detection of SARS-CoV-2 variants (D614G mutation) and direct detection of bacterial 16S rRNA.
Introduction: D614G), conferring greater infectivity and more rapid spread, have become predominant in various regions.
Introduction: Moreover, STAR was utilized for multiplex detection of the D614G mutation and N gene of SARS-CoV-2 in a single tube as well as for the direct detection of bacterial 16S rRNA without additional nucleic acid purification, thereby confirming the wide applicability of this method for nucleic acid biomarker detection.
Figure: (A) Illustration of the STAR assay for the D614G mutation in the S gene of SARS-CoV-2.
Figure: (C) Lim
Efficacy of vaccination and previous infection against the Omicron BA.1 variant in Syrian hamsters.
Introduction: Here, in the hamster model, we addressed these issues under controlled conditions using the Omicron variant and isolates that are no longer circulating in nature (i.e., a Wuhan-like isolate and an isolate with only a D614G spike mutation).
Mutation rate of SARS-CoV-2 and emergence of mutators during experimental evolution.
PMID: 35419205
2022
Evolution, medicine, and public health
Conclusion: Assuming that the D614G genotype is better adapted than its ancestor in Vero cells, this observation is consistent with the hypothesis that fitter genotypes adapt at a slower pace.
Conclusion: Here, we followed the evolution in cells of two strains of SARS-CoV-2: one with the original spike protein (CoV-2-D) and one carrying the D614G mutation (CoV-2-G), a variant that emerged in the early phase of the pandemic and soon became prevalent.
Conclusion: The two genomic backgrounds shared several de novo mutations, suggesting that the D614G mutation on the spike protein did not
Result: This D614G mutation in the spike protein emerged early in the pandemic, increased the infectivity of the virus and became prevalent worldwide.
Pathogenicity of SARS-CoV-2 Omicron (R346K) variant in Syrian hamsters and its cross-neutralization with different variants of concern.
Method: On deep sequencing following amino acid changes were found in the isolate.(NSP5_P132H,Spike_T95I,Spike_K417N,Spike_S373P,Spike_Q493R,Spike_N969K, Spike_H655Y,Spike_N856K,N_R203K,Spike_S371L,NSP3_A1892T, PMID: 35403837
2022
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Abstract: Pseudotyped and authentic virus-based assays show that COVID-HIG displays broad-spectrum neutralization effects on a wide variety of SARS-CoV-2 variants, including D614G, Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Kappa (B.1.617.1), Delta (B.1.617.2), and Omicron (B.1.1.529) in vitro.
Inhibitor screening using microarray identifies the high capacity of neutralizing antibodies to Spike variants in SARS-CoV-2 infection and vaccination.
3Introduction: For example, the D614G variant, which was first identified in July 2020, has a faster infection rate and higher viral load in the upper respiratory tract than the wild-type ""Wuhan-Hu-1"" strain."
Abstract: In addition, similar results were obtained using a SARS-CoV-2 pseudovirus neutralization assay specific for wild-type S and five prevalent S variants (D614G, B.1.1.7, B.1.351, P.1, B.1.617.2), thus demonstrating that high antibody diversity is associated with high NAb titers.
Introduction: The B.1.1.7 variant (D614G, N501Y) is more infectious and may lead to increased mortality compared to the parental strain.
Result: Consistent with the observation that the trimerized form of the D614G PMID: 35346184
2022
BMC medicine
Method: SARS-CoV-2-pseudotyped lentiviruses were prepared by co-transfecting the HEK 293T cell line with either the SARS-CoV-2 original spike (D614G) or the SARS-CoV-2 beta or delta spike plasmids in conjunction with a firefly luciferase encoding pNL4 lentivirus backbone plasmid.
Method: The RBD proteins were derived from the original D614G strain and the four variants of concern, namely alpha, beta, gamma, and delta.
Method: The SARS-CoV-2 original (D614G) spike and RBD proteins were expressed in human embryonic kidney (HEK) 293F suspension cells by transfecting the cells with the spike plasmid.
Figure: D Magnitude of neutralisation activity against the beta, <
High-resolution melting analysis after nested PCR for the detection of SARS-CoV-2 spike protein G339D and D796Y variations.
PMID: 35349821
2022
Biochemical and biophysical research communications
Introduction: reported the detection of D614G (nucleotide mutation: A23403G) and L452R (nucleotide mutation: T22917G) variations in the SARS-CoV-2 spike protein by post-PCR HRM analysis, respectively.
Discussion: In the case of detection for the D614G variation (mutation A23403G) reported by Gazali et al., the difference in the Tm value was 0.23 C.
Evasion of vaccine-induced humoral immunity by emerging sub-variants of SARS-CoV-2.
Introduction: As vaccine development was initiated almost immediately after the pandemic started, current major vaccines, including BNT162b2, mRNA1273 and ChAdOx1-S, are all based on the original strain without D614G.
Introduction: One of the salient mutations, spike-D614G, appeared in January 2020 and soon became ubiquitously dominant by April.
SARS-CoV-2-specific antibody and T-cell responses 1 year after infection in people recovered from COVID-19: a longitudinal cohort study.
Result: 21 (75%) of 28 patients with a titre of 1/10 to 1/20 (p=0 047), 21 (49%) of 43 patients with a titre of 1/20 to 1/32 (p<0 0001), and five (11%) of 44 patients with a titre of 1/32 or more (p=0 0001) lost neutralising activity to the D614G variant at 12 months.
Result: By contrast, only 68 (48%) had neutralising antibodies against D614G, 32 (23%) had neutralising antibodies against the beta variant, and 69 (49%) had neutralising antibody responses against the delta variant (all p<0 0001; figure 4A ).
Result: Moreover, the neutralising antibody titres against the D614G and delta variants were similar (p=0 42), and both were higher than those against the beta variant (p=0 036 for D614G vs beta and p=0 0019 for delta vs beta; figure 4A).
Result: The D614G (p=0 36) and beta (p=0 82) variants escape
SARS_CoV_2 mutation literature information.
PMID: 
2022
Biosensors & bioelectronics
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