Introduction: And compared to the original strain of SARS-CoV-2, the D614G mutation makes the S protein more stable and more flexible, which makes the virus more infectious.
Introduction: For example, S protein of the D614G variants has a looser and wider trimer structure of RBD.
Introduction: In July 2020, it was reported that the strain with the spike protein D614G mutation in Europe is more contagious and may become the main form of the virus pandemic.
Result: In addition, Alpha, Beta, Delta, and Omicron strains had partial protruding structures at the junction of S protein and virus particles (Figure 1), which may be caused by the internal extrusion of the S p
Durability and Cross-Reactivity of SARS-CoV-2 mRNA Vaccine in Adolescent Children.
1Abstract: We tested the durability and cross-reactivity of anti-SARS-CoV-2 serologic responses over a six-month time course in vaccinated adolescents agains
3Introduction: Here, we quantified relative antibody responses in adolescent children immediately following the Pfizer-BioNTech mRNA vaccination and six months post-inoculation and analyzed the efficacy of the humoral response against the D614G (""wild type"") SARS-CoV-2 and latest variant of concern (VOC), Omicron."
4Method: Serological analyses were performed using an in-house enzyme-linked immunosorbent assay (ELISA) that detects IgG against the D614G (""wild type"") SARS-CoV-2 Spike, the D614G (""wild type"") Receptor-Binding Domain (RBD), or the Omicron SARS-CoV-2 VOC RBD by using the previously described method."
Comparative Evaluation of Six SARS-CoV-2 Real-Time RT-PCR Diagnostic Approaches Shows Substantial Genomic Variant-Dependent Intra- and Inter-Test Variability, Poor Interchangeability of Cycle Threshold and Complementary Turn-Around Times.
Abstract: We comparatively assessed the variability of Ct values generated by six diagnostic approaches by testing serial dilutions of well-characterized isolates of 10 clinically most relevant SARS-CoV-2 genomic variants: Alpha, Beta, Gamma, Delta, Eta, Iota, Omicron, A.27, B.1.258.17, and B.1 with D614G mutation.
Introduction: The main aim of this study was to comparatively assess the variability of Ct values generated by six previously thoroughly evaluated rtRT-PCR assays and platforms by testing serial dilutions of well-characterized SARS-CoV-2 isolates of the clinically most relevant genomic variants (Alpha, Beta, Gamma, Delta, Eta, Iota, Omicron, A.27, B.1.258.17, and B.1 with D614G mutation).
Discussion: In addition, variant B.1.258.17 as the main lineage during the second wave, variant A.27 as a rare variant, with mutation N501Y, and variant B.1 (with
Genome Profiling of SARS-CoV-2 in Indonesia, ASEAN and the Neighbouring East Asian Countries: Features, Challenges and Achievements.
Result: All B.1.466.2 and B.1.470 variants in Indonesia carried the D614G spike protein mutation, as do the other VoCs (Table 3).
Result: As well as a B.1.1 variant, all 12 genomes share common mutations in the spike protein D614G and NSP12_P323L (Table S1).
Result: In Indonesia, this P323L mutation was found in all B.1.466.2, B.1.1.7, and B.1.617.2 variants (Table 3) and was co-present with the D614G mutation in many genomes in the world.
Highly Thermotolerant SARS-CoV-2 Vaccine Elicits Neutralising Antibodies against Delta and Omicron in Mice.
Result: For monomeric formulations the fold reduction values were 2.5 and 14.4 and for trimeric 3.0 and 16.5, to Delta and Omicron, respectively, compared to VIC31-D614G.
Result: Neutralisation assays were performed using VIC31-D614G, Delta and Omicron SARS-CoV-2 variants.
Result: The mean antibody titres to VIC31-D614G following immunisation with the vaccine formulations comprising antigens presented as monomers (Vaccines 1-3) showed a statistically non-significant (p < 0.1) increase when compared to the trimers (Vaccines 4-6) (Figure 3J-L; Table 3).
Result: The reduction in neutralisation of Omicron was more pronounced, ranging from a 10.1- to 22.0-fold decrease for the six vaccine formulations, when compared to VIC31-D614G and was statistically significant (p < 0.01) for all.
Result: There was a reduction in neutralising tit
Functional Analysis of Spike from SARS-CoV-2 Variants Reveals the Role of Distinct Mutations in Neutralization Potential and Viral Infectivity.
Introduction: This process is driven by both escape from neutralizing antibodies, where mutations like E484K, E484Q, S477N play key rol
Result: Initially, single point FCS mutations were inserted into the Wuhan wild-type (D614G) spike.
Discussion: These reports demonstrate that spike residues like D614G and N501Y are located at the distal region of the spike RBD and facilitate transitions from a closed to open state of the spike prior to ACE2 binding, enhancing the stability and affinity of the viral spike to its receptor and subsequently affecting viral spread.
Increased resistance of SARS-CoV-2 Lambda variant to antibody neutralization.
Result: Analysis of two other Class 3 antibodies, REGN10987 and S309, showed that neither recognized the L452 and F490 residuals directly but each still neutralized the Lambda variant with similar potencies as those against D614G-WT.
Result: Compared with the WT strain (D614G-WT), both Lambda variant and L452Q/F490S mutated virus reduced the susceptibility by approximately 50%.
Result: SARS-CoV-2 Lambda variant harbored G75V, T76I, R246N, and Delta246-252 mutations in the N terminal domain, L452Q and F490S in the RBD, D614G and T859N
SARS-CoV-2 mutations acquired during serial passage in human cell lines are consistent with several of those found in recent natural SARS-CoV-2 variants.
PMID: 35474907
2022
Computational and structural biotechnology journal
Introduction: For example, the D614G and P681R mutations in the spike protein were associated with increased viral replication in human lung epithelial cells and enhanced viral pathogenicity, respectively.
Discussion: Following the noticeable increase in the D614G mutation in April 2020, the Omicron variant has acquired over 30 mutations, including those found in the Alpha, Beta, Gamma, and Delta variants.
Discussion: However, in this experiment, key natural mutations such as D614G were not observed, which could be due to the specific host cells used or the in vitro culture system.
Safety and Immunogenicity of Inactivated COVID-19 Vaccines Among People Living with HIV in China.
Discussion: However, the GMT for the delta variant was significantly lower than the GMT for the D614G variant in PLWH, which is consistent with Chang Liu's finding that mRNA vaccines induced an antibody response to some variants, but the neutralization of the delta variant was reduced.
Discussion: SARS-CoV-2 IgG concentrations and neutralizing antibody titers against the D614G and delta variants also declined significantly in PLWH compared to HDs.
Discussion: The D614G and delta variants harbor mutations in the RBD, so neutralizing titers to the pseudotyped virus may better show the immune response to the two variants elicited by vaccination than titers of S-RBD-IgG.
Discussion: The positive rates of neut
Biomechanical Dependence of SARS-CoV-2 Infections.
Abstract: Given the recent data highlighting the importance of alternative virulent strains, we included both the native strain identified in early 2020 and an early S protein variant (D614G) that was shown to increase the viral infectivity markedly.
Abstract: Our results show that cells on softer and sparser scaffolds, closer resembling younger lungs, exhibit higher infection rates by the WT and D614G variant.
SARS_CoV_2 mutation literature information.
PMID: 
2022
MedComm
Browser Board
Co-occurred Entities
Filtrator
ViMRT