Abstract: Based on previous reports and our observations, we can conclude that the occurrence of one of two mutations
Figure: Abbreviations stand for: CT, cytoplasmic domain; D614G, mutation in the S protein; F486L, mutation in the S protein; FP, fusion peptide; HR1, heptapeptide repeat sequence 1; HR2, heptapeptide repeat sequence 2; N501T, mutation in the S protein; NDT, N-terminal domain; PRRA, polybasic cleavage site; RBD, receptor-binding domain; S1, S1 subunit of the S protein; S2, S2 subunit of the S protein; TM, transmembrane domain; Y453F, mutation in the S protein.
Dynamic Ca(2+) sensitivity stimulates the evolved SARS-CoV-2 spike strain-mediated membrane fusion for enhanced entry.
Result: A previous study reported that D614G allows more open conformation of the RBD domain of spike, which may facilitate ACE2 binding.
Result: Although our experiments do not measure direct binding of spike and receptor/co-receptor (just consequences of the binding) and receptor engagement is only one interpretation, a D614G spike might be more prone to triggering as a result of binding, or the mutant spike might be more likely to engage the target membrane as a result of the same triggering.
Result: Both the Alpha and Beta spike variants evolved to be more sensitive to calcium concentration dynamics and show higher fusion activity compared to PMID: 35398519
2022
Clinical immunology (Orlando, Fla.)
Introduction: Even in the early stage of the pandemic, the D614G mutation increased and replaced the previous strain within a few months; however, since this mutation was outside the RBD, it did not h
Result: Because variant strains such as Alpha variant have been reported in Japan since January 2021, and because this study used samples from patients who had been infected by COVID-19 before that time, all patients were thought to be infected with the Wuhan-hu-1 strain or the strain with D614G.
Discussion: There are several limitations in this study: first, because most of the participants were infected by the Wuhan-hu-1 strain with or without D614G mutation, we cannot assess immune evasion among other strains, such as the neutralization titers of the Delta variant after infection with the Alpha variant.
Inhibitor screening using microarray identifies the high capacity of neutralizing antibodies to Spike variants in SARS-CoV-2 infection and vaccination.
Abstract: In addition, similar results were obtained using a SARS-CoV-2 pseudovirus neutralization assay specific for wild-type S and five prevalent S variants (D614G, B.1.1.7, B.1.351, P.1, B.1.617.2), thus demonstrating that high antibody diversity is associated with high NAb titers.
Result: Consistent with the observation that the trimerized form of the D614G S protein has an increased binding affinity to ACE2, we also found the D614G mutation modestly improved the binding of S-ACE2 in this study.
Result: Like the convalescent COVID-19 patients, the D614G variant did not alter the neutralizing effect of the NAbs compared to wild-type S1+S2, S2, and PMID: 35403837
2022
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Abstract: Pseudotyped and authentic virus-based assays show that COVID-HIG displays broad-spectrum neutralization effects on a wide variety of SARS-CoV-2 variants, including D614G, Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Kappa (B.1.617.1), Delta (B.1.617.2), and Omicron (B.1.1.529) in vitro.
Pathogenicity of SARS-CoV-2 Omicron (R346K) variant in Syrian hamsters and its cross-neutralization with different variants of concern.
Method: On deep sequencing following amino acid changes were found in the isolate.(NSP5_P132H,Spike_T95I,Spike_K417N,Spike_S373P,Spike_Q493R,Spike_N969K, Spike_H655Y,Spike_N856K,N_R203K,Spike_S371L,NSP3_A1892T, PMID: 35419205
2022
Evolution, medicine, and public health
Abstract: Methodology: We performed experimental evolution with two strains of SARS-CoV-2, one carrying the originally described spike protein (CoV-2-D) and another carrying the D614G mutation that has spread worldwide (CoV-2-G).
Conclusion: Assuming that the D614G genotype is better adapted than its ancestor in Vero cells, this observation is consistent with the hypothesis that fitter genotypes adapt at a slower pace.
Conclusion: Here, we followed the evolution in cells of two strains of SARS-CoV-2: one with the original spike protein (CoV-2-D) and one carrying the D614G mutation (CoV
Result: This D614G mutation in the spike protein emerged early in the pandemic, increased the infectivity of the virus and became prevalent worldwide.
Efficacy of vaccination and previous infection against the Omicron BA.1 variant in Syrian hamsters.
Introduction: Here, in the hamster model, we addressed these issues under controlled conditions using the Omicron variant and isolates that are no longer circulating in nature (i.e., a Wuhan-like isolate and an isolate with only a D614G spike mutation).
Split T7 promoter-based isothermal transcription amplification for one-step fluorescence detection of SARS-CoV-2 and emerging variants.
Conclusion: Finally, (v) broad applicability was verified through the multiplex detection of the SARS-CoV-2 variants (D614G mutation) as well as through the direct detection of bacterial 16S rRNA without the need for additional nucleic acid purification.
Introduction: D614G), conferring greater infectivity and more rapid spread, have become predominant in various regions.
Introduction: Moreover, STAR was utilized for multiplex detection of the D614G mutation and N gene of SARS-CoV-2 in a single tube as well as for the direct detection of bacterial 16S rRNA without additional nucleic acid purification, thereby confirming the wide applicability of this method for nucleic acid biomarker detection.
Result: 5B indicate that G at N4 minimized the background noise in the presence of the wild-type target while generating a high fluorescence sign
Comparative genomics, evolutionary epidemiology, and RBD-hACE2 receptor binding pattern in B.1.1.7 (Alpha) and B.1.617.2 (Delta) related to their pandemic response in UK and India.
PMID: 35427787
2022
Infection, genetics and evolution
Abstract: First, we served comparative genomics, such as genome sequence submission patterns, mutational landscapes, and structural landscapes of significant mutations (N501Y, D614G, L452R, E484Q, and P681R).
Abstract: The structural pattern was analyzed in the N501Y, D614G L452R, E484Q, and P681R mutations.
Result: Again, like the previous point AA mutation, we also evaluated the D614G mutation, which was identified as the B.1.1.7 variant.
Result: In the D614G structure, the amino acid changed from Asp614 Gly.|mg
SARS_CoV_2 mutation literature information.
PMID: 
2022
Journal of applied genetics
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