Introduction: In context to the same, here we assessed the antigenic propensity of the epitope encompassing the D614G mutation considering its high frequency and the segment being earlier reported as immune-dominant peptide in SARS-CoV.
Introduction: There are a lot of variations observed in spike protein sequence, a major variation in spike protein is a non-synonymous D614G mutation (d-Aspartate, G-Glycine) which has received special attention by several groups due to its dominance.
Resu
Result: A total of 34 nonsynonymous mutations were observed, with the spike D614G having the highest occurrence in 2400 genomes followed by the nsp12 P4715L mutation observed in 2254 genomes (Figure 1A).
Production, Titration, Neutralisation, Storage and Lyophilisation of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Lentiviral Pseudotypes.
Abstract: This protocol details a rapid and reliable method for the production and titration of high-titre viral pseudotype particles with the SARS-CoV-2 spike protein (and D614G or other variants of concern, VOC) on a lentiviral vector core, and use for neutralisation assays in target cells expressing angiotensin-converting enzyme 2 (ACE2) and transmembrane serine protease 2 (TMPRSS2).
Characterization of altered genomic landscape of SARS-CoV-2 variants isolated in Saudi Arabia in a comparative in silico study.
PMID: 34866979
2021
Saudi journal of biological sciences
Abstract: The most frequently changed nucleotide was C3037T (silent mutation) and A23403G (D614G), each of which occurred in 57 variants out of 58 followed by C14408T (P4715L) and C241T (5'UTR) which were found in 56 and 55 variants respectively.
Result: Our analysis showed that the most frequently changed nucleotide were C3037T (silent mutation) and A23403G (D614G) each of which occurred in 57 variants out of 58, followed by C14408T (P4715L) and C241T (5'UTR) which were found in 56
Site-Specific Glycosylation Patterns of the SARS-CoV-2 Spike Protein Derived From Recombinant Protein and Viral WA1 and D614G Strains.
Abstract: Comparison of the whole cell-derived WA1 and D614G spike proteins revealed that N-glycosites local to the mutation site appeared to be more readily detected, hinting that these sites are more exposed to glycosylation machinery.
Abstract: Moreover, recombinant HEK293-derived S1 was occupied almost completely with complex glycan, while both WA1 and D614G derived from the Vero E6 cell whole virus were predominantly high-mannose glycans.
Abstract: The Vero cell-derived spike from the WA1 strain and a D614G variant was analyzed.
C
Conclusion: Glycan modification at most N-glycosites is very similar between WA1 and D614G and primarily high-mannose, with significant differences at N343.
Arterial and Venous Thrombosis Complicated in COVID-19: A Retrospective Single Center Analysis in Japan.
PMID: 34869681
2021
Frontiers in cardiovascular medicine
Discussion: Recently, several reports described the promotion of thrombus formation via the activation of platelets, the induction of tissue factor, or PAI-1 using virus particle of the Wuhan strain or recombinant S1 protein with D614G single mutation.
SARS-CoV-2 genetic variations associated with COVID-19 pathogenicity.
Introduction: the mutation D614G in the virus spike protein), which tended to be found together, were reported to show significant positive correlations with death, and to be found more frequently in severe cases than mild cases.
Table: D614G
Mutational landscape of SARS-CoV-2 genome in Turkey and impact of mutations on spike protein structure.
Introduction: One of the first variations that spread vigorously across countries was Asp614Gly at the spike protein, with this mutation showing higher viral loads than the reference virus from Wuhan, China.
Discussion: A Consurf analysis indicated that all the mutations were located in variable regions of the protein except for Asp614Gly.
Discussion: A comparison of surface hydrophobicity of the trimeric spike reference protein with the Asp614Gly mutation over the simulation trajectory indicated surface hydrophobicity changed for both proteins.
Discussion: On the other hand, the Asp614Gly missense mutation, which has been widely detected in different regions of the world, was seen in two (ACUTG-1 and ACUTG-5) out of the genome sequences of 13 patients in our cohort.|
N-glycosylation profiles of the SARS-CoV-2 spike D614G mutant and its ancestral protein characterized by advanced mass spectrometry.
Conclusion: The results of this study revealed that the single D614G substitution had impacts on the glycosylation of the spike protein and half of the N-glycosylation sequons in the S showed a difference in the distribution of various glycan forms between the S-614D and S-614G variants.
Conclusion: We analyzed the N-glycosylation profiles of the SARS-CoV-2 spike D614G variant and its ancestor protein using advanced EThcD mass spectrometry.
Introduction: from molecular dynamic (MD) studies on the variant sequence and was supported by a recent cryo-EM study of S-
Table: D614G
Reduced neutralization of SARS-CoV-2 B.1.617 variant by convalescent and vaccinated sera.
Abstract: Our results showed that, compared to D614G and B.1.1.7 variants, B.1.617 shows enhanced viral entry and membrane fusion, as well as more resistant to antibody neutralization.
Analysis of the ARTIC Version 3 and Version 4 SARS-CoV-2 Primers and Their Impact on the Detection of the G142D Amino Acid Substitution in the Spike Protein.
Introduction: bearing resemblance to previous evolutionary sweeps, including the D614G substitution in 2020, B.1.1.7 (Alpha) last fall and winter, and Delta this spring and summer (GISAID acknowledgment table can be found at doi: https://doi.org/10.1101/2021.09.27.461949).
SARS_CoV_2 mutation literature information.
PMID: 
2021
Frontiers in genetics
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