Role of the Microbiome in the Pathogenesis of COVID-19.
PMID: 35433495
2022
Frontiers in cellular and infection microbiology
Abstract: Soon after the first outbreak due to the wild-type strain in December 2019, a genetic variant D614G emerged in late January to early February 2020 and became the dominant genotype worldwide.
Conclusion: The two most commonly occurring mutations, Spike_D614G and Nsp12_P314L, were structurally modeled, which showed that these mutations had the potential to enhance viral entry and replication, respectively.
Introduction: Among these, the D614G clade was the most common and was first found in late January 2020 in China, according to a study conducted by.
Introduction: Earliest samples from the USA appeared to have been derived from China and belonged to basal or L84S
Table: D614G
Antigenicity comparison of SARS-CoV-2 Omicron sublineages with other variants contained multiple mutations in RBD.
Abstract: Among them, D614G, B.1.640.1, and B.1.630 formed a cluster, C.1.2 and B.1.640.2 formed a cluster, and BA.1, BA.2, and BA.3 formed a cluster.
Method: D614G (GISAID: EPI_ISL_766872), BA.1 (GISAID: EPI_ISL_6590782.2), BA.2 (GISAID: EPI_ISL_7644798), BA.3 (GISAID: EPI_ISL_7740765), C.1.2 (GISAID: EPI_ISL_8801147), B.1.630 (GISAID: EPI_ISL_6368831), B.1.640.1 (GISAID: EPI_ISL_8013598), and B.1.640.2 (GISAID: EPI_ISL_8376567) spike protein expression plasmids are all entrusted to General Biology (Anhui) Co., Ltd.
Method: Briefly, 293T cells were first transfected with the SARS-CoV-2 spike protein expression plasmids of D614G or VOCs/VOIs (Alpha, Beta, Gamma, Delta, Lambda, Mu, and Omicron).
Method: Each guinea pig was subcutaneously immunized with 100 mug of purified spik
Characteristic analysis of Omicron-included SARS-CoV-2 variants of concern.
Discussion: In July 2020, a strain with the spike protein D614G mutation was discovered in Europe and subsequently became the main form of the virus pandemic.
Discussion: In the Alpha and Omicron strains, two key deletions, H60V70, are on the epitope, which can affect the immune escape of the S protein (Figure 4F), whereas N501Y and D614G have almost no effect on the antigen score, and the P681 mutation slightly reduces the epitope score and may have a certain impact.
Discussion: The RBD regions of Alpha, Beta, Delta, and Omicron strains are more exposed, and the junction between the variants S protein and the virion has a prominent structure, which may be caused by the D614G mutation.
Durability and Cross-Reactivity of SARS-CoV-2 mRNA Vaccine in Adolescent Children.
1Abstract: We tested the durability and cross-reactivity of anti-SARS-CoV-2 serologic responses over a six-month time course in vaccinated adolescents agains
3Introduction: Here, we quantified relative antibody responses in adolescent children immediately following the Pfizer-BioNTech mRNA vaccination and six months post-inoculation and analyzed the efficacy of the humoral response against the D614G (""wild type"") SARS-CoV-2 and latest variant of concern (VOC), Omicron."
4Method: Serological analyses were performed using an in-house enzyme-linked immunosorbent assay (ELISA) that detects IgG against the D614G (""wild type"") SARS-CoV-2 Spike, the D614G (""wild type"") Receptor-Binding Domain (RBD), or the Omicron SARS-CoV-2 VOC RBD by using the previously described method."
The twin-beginnings of COVID-19 in Asia and Europe-one prevails quickly.
Abstract: The first wave is a group of four mutations (C241T, C3037T, C14408T and A23403G [this being the amino acid change D614G]; all designated 0 to 1 below).
Abstract: This DG (D614G) group, fixed at the start of the pandemic, is the foundation of all subsequent waves of strains.
Biomechanical Dependence of SARS-CoV-2 Infections.
Abstract: Given the recent data highlighting the importance of alternative virulent strains, we included both the native strain identified in early 2020 and an early S protein variant (D614G) that was shown to increase the viral infectivity markedly.
Abstract: Our results show that cells on softer and sparser scaffolds, closer resembling younger lungs, exhibit higher infection rates by the WT and D614G variant.
RBD-mRNA vaccine induces broadly neutralizing antibodies against Omicron and multiple other variants and protects mice from SARS-CoV-2 challenge.
Abstract: The vaccine induced durable antibodies that potently neutralized prototypic strain and B.1.1.7 lineage variant pseudoviruses containing N501Y or D614G mutations alone or in combination with a N439K mutation (B.1.258 lineage), with a L452R mutation (B.1.427 or B.1.429 lineage), or a L452R-E484Q double mutation (B.1.617.1 variant), although neutralizing activity against B.1.1.7 lineage variant containing 10 amino acid changes in the S protein was slightly reduced.
Safety and Immunogenicity of Inactivated COVID-19 Vaccines Among People Living with HIV in China.
Discussion: However, the GMT for the delta variant was significantly lower than the GMT for the D614G variant in PLWH, which is consistent with Chang Liu's finding that mRNA vaccines induced an antibody response to some variants, but the neutralization of the delta variant was reduced.
Discussion: SARS-CoV-2 IgG concentrations and neutralizing antibody titers against the D614G and delta variants also declined significantly in PLWH compared to HDs.
Discussion: The D614G and delta variants harbor mutations in the RBD, so neutralizing titers to the pseudotyped virus may better show the immune response to the two variants elicited by vaccination than titers of S-RBD-IgG.
Discussion: The positive rates of neut
Whole genome sequencing of SARS-CoV2 strains circulating in Iran during five waves of pandemic.
Abstract: There were different mutations in all parts of the genomes but Spike-D614G, NSP12-P323L, N-R203K and N-G204R were the most frequent mutants in these studied viruses.
Delta variant (B.1.617.2) of SARS-CoV-2: Mutations, impact, challenges and possible solutions.
PMID: 35507895
2022
Human vaccines & immunotherapeutics
Abstract: The enhanced transmissibility of Delta variant has been associated with critical mutations such as D614G, L452R, P681R, and T478K in the S-protein.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Frontiers in cellular and infection microbiology
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