The dynamics of circulating SARS-CoV-2 lineages in Bogor and surrounding areas reflect variant shifting during the first and second waves of COVID-19 in Indonesia.
Discussion: Although not associated with worse disease severity, the S_D614G change has been implicated in enhanced transmission and higher viral loads .
Discussion: For Delta variants, we observed 12 key amino acid changes in spike protein that were mostly similar to other studies, including S_T19R, S_T95I, S_G142D, S_E156-, S_F157-, S_R158G, S_K417N, S_L452R, S_T478K, S_D614G, S_P681R, and S_D950N .
Discussion: On the other hand, substitutions in the region encoding the spike protein in Indonesian lineages mainly consisted of thr
SARS-CoV-2-specific antibody and T-cell responses 1 year after infection in people recovered from COVID-19: a longitudinal cohort study.
Result: 21 (75%) of 28 patients with a titre of 1/10 to 1/20 (p=0 047), 21 (49%) of 43 patients with a titre of 1/20 to 1/32 (p<0 0001), and five (11%) of 44 patients with a titre of 1/32 or more (p=0 0001) lost neutralising activity to the D614G variant at 12 months.
Result: By contrast, only 68 (48%) had neutralising antibodies against D614G, 32 (23%) had neutralising antibodies against the beta variant, and 69 (49%) had neutralising antibody responses against the delta variant (all p<0 0001; figure 4A ).
Result: Moreover, the neutralising antibody titres against the D614G and delta variants were similar (p=0 42), and both were higher than those against the beta variant (p=0 036 for D614G vs beta and p=0 0019 for delta vs beta; figure 4A).
Result: The D614G (p=0 36) and beta (p=0 82) variants escape
AstraZeneca COVID-19 vaccine induces robust broadly cross-reactive antibody responses in Malawian adults previously infected with SARS-CoV-2.
Method: SARS-CoV-2-pseudotyped lentiviruses were prepared by co-transfecting the HEK 293T cell line with either the SARS-CoV-2 original spike (D614G) or the SARS-CoV-2 beta or delta spike plasmids in conjunction with a firefly luciferase encoding pNL4 lentivirus backbone plasmid.
Method: The RBD proteins were derived from the original D614G strain and the four variants of concern, namely alpha, beta, gamma, and delta.
Method: The SARS-CoV-2 original (D614G) spike and RBD proteins were expressed in human embryonic kidney (HEK) 293F suspension cells by transfecting the cells with the spike plasmid.
Figure: D Magnitude of neutralisation activity against the beta, <
High-resolution melting analysis after nested PCR for the detection of SARS-CoV-2 spike protein G339D and D796Y variations.
PMID: 35349821
2022
Biochemical and biophysical research communications
Introduction: reported the detection of D614G (nucleotide mutation: A23403G) and L452R (nucleotide mutation: T22917G) variations in the SARS-CoV-2 spike protein by post-PCR HRM analysis, respectively.
Discussion: In the case of detection for the D614G variation (mutation A23403G) reported by Gazali et al., the difference in the Tm value was 0.23 C.
Evasion of vaccine-induced humoral immunity by emerging sub-variants of SARS-CoV-2.
Introduction: As vaccine development was initiated almost immediately after the pandemic started, current major vaccines, including BNT162b2, mRNA1273 and ChAdOx1-S, are all based on the original strain without D614G.
Introduction: One of the salient mutations, spike-D614G, appeared in January 2020 and soon became ubiquitously dominant by April.
Differential neutralizing antibody responses elicited by CoronaVac and BNT162b2 against SARS-CoV-2 Lambda in Chile.
Abstract: Compared with the ancestral virus, neutralization against D614G, Alpha, Gamma, Lambda and Delta variants was reduced by between 0.93- and 4.22-fold for CoronaVac, 1.04- and 2.38-fold for BNT162b2, and 1.26- and 2.67-fold for convalescent plasma.
Mutational cascade of SARS-CoV-2 leading to evolution and emergence of omicron variant.
Abstract: Mutational analysis detected 18,261 mutations in the omicron variant, majority of which were non-synonymous mutations in spike (A67, T547K, D614G, H655Y, N679K, P681H, D796Y, N856K, Q954H), followed by RNA dependent RNA polymerase (rdrp) (A1892T, I189V, P314L, K38R, T492I, V57V), ORF6 (M19M) and PMID: 35369437
2022
Frontiers in microbiology
Result: To other sequences except 200327-84, 200328-142 and 200328-143, the D614G amino acid change in the S protein occurred by an A23403G nucleotide mutation, which confirmed to be associated with greater infectivity (Figure 2B).
Discussion: D614G mutation can be found in most of our genome sequences, which is consistent with the global variation trend of SARS-CoV-2.
Discussion: D614G, in the S protein of SARS-CoV-2, which has emerged as a predominant clade in Europe and was spreading worldwide.
Discussion: demonstrated that D614G has enhanced binding to the ACE2, increased replication in human bronchial and nasal airway epithelial cultures and in a human ACE2 knock-in mouse model, and increased replication and transmission in S
Atorvastatin Effectively Inhibits Ancestral and Two Emerging Variants of SARS-CoV-2 in vitro.
Abstract: In addition, ATV showed an antiviral effect against the D614G strain independent of the cell line (EC50 of 7.4 muM in Caco-2).
Abstract: Our study demonstrated the in vitro antiviral activity of atorvastatin against the ancestral SARS-CoV-2 D614G strain and two emerging variants (Delta and Mu), with an independent effect of the cell line.
Abstract: This compound showed antiviral activity against SARS-CoV-2 D614G strain in Vero E6 with median effective concentrations (EC50s) of 15.4, 12.1, and 11.1 muM by pre-post, pre-infection, and post-infection treatments, respectively.
Result: ATV Exhibited Antiviral Effects Against SARS-CoV-2 Result: As shown in Figure 6, ATV inhibited significantly the SARS-CoV-2 D614G strain in Caco-2 at 7.8 muM (52.7%, p = 0.03), by pre-post treatment (EC50 = 7.4 muM, SI = 8.7).
Impact of B.1.617 and RBD SARS-CoV-2 variants on vaccine efficacy: An in-silico approach.
PMID: 35370005
2022
Indian journal of medical microbiology
Discussion: D614G, T20N, D138Y, L18F, R190S, and P26S in the NTD and K417T, E484K and N501Y in the RBD region and H655Y within the furin cleavage site.
Discussion: The mRNA-1273 vaccine's neutralizing activity towards number of variants like B.1.351, B.1.1.7 + E484K, B.1.1.7, P.1, B.1.427/B.1.429, D614G, 20A.EU2, 20E [EU1], N439K-D614G, and previously identified mutant in Denmark mink cluster 5 were identified and found to have the same neutrality level as Wuhan-Hu-1
SARS_CoV_2 mutation literature information.
PMID: 
2022
PeerJ
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