Result: Additionally, in March when the D614G mutation became predominant in Japan, there was an increase of cough searches much greater than the increase of searches of fever that continued in April (Fig 4F).
Result: Because we observed that symptom order changes by viral variant between the initial outbreak in China and the subsequent outbreak in the USA and in Japan and studies link the D614G mutation with changes in disease pathology, we hypothesized that these changes in likely symptom order are not due to comorbidities of the patients.
Result: Furthermore, the magnitudes of these changes indicate that more people were searching these terms in March 2020 than the previous year, as the number of D614G patients increased throughout Japan.
Result: Given the evidence that the D614G variant affects symptom order, we tested the effect of
Structural analysis of receptor binding domain mutations in SARS-CoV-2 variants of concern that modulate ACE2 and antibody binding.
Result: Additionally, the positioning of K31 within ACE2 is shifted relative to the D614G spike, adopting a position within pi-cation bonding distance to Y489 within the RBD (Figure S4M).
Result: Analysis of the D614G + N501Y + E484K mutant
Figure: (B) Area under the curve (AUC) fold changes in ELISA binding assays relative to D614G alone for Ab1, Ab8, CR3022, S309, and S2M11.
Figure: (B) Relative fold change differences in S protein-ACE2 affinity (Kd) relative to D614G alone.
Figure: All constructs contain the D614G mutation as background, and this was defined as the wild-type construct throughout the study.
Case Report: Genomic Characteristics of the First Known Case of SARS-CoV-2 Imported From Spain to Sichuan, China.
Discussion: Although it has been found that SARS-CoV-2 with the D614G mutation in the spike protein can replicate and spread faster than strains without this mutation, interestingly, the P323L mutation has been reported to co-evolve with D614G worldwide, this adaptation of the virus might strengthen SARS-CoV-2 G614 strain replication rates and infectivity.
Discussion: In summary, we sequenced and reported the first case of mutations in the genes encoding the spike (D614G) and Nsp12 (P323L) proteins of SARS-CoV-2 in Sichuan Province, China.
Discussion: These changes translated to four amino acid changes in three proteins: spike (D614G
Heterologous prime-boost immunizations with chimpanzee adenoviral vectors elicit potent and protective immunity against SARS-CoV-2 infection.
Method: pS-B.1.1.7 and pS-B.1.351 plasmids were constructed with mutant S genes expressing the spike protein of the B.1.1.7 variant (GenBank: QQH18545.1, containing the H69, V70, and Y145 deletions and N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H mutations) and B.1.351 variant (GenBank: QRI43207.1, containing the L242, A243, and L244 deletions and L18F, D80A, D215G, S305T, K417N, E484K, N501Y, D614G
Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein Elicits Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants.
Introduction: The Alpha (B.1.1.7) variant encodes an S protein with nine mutations (del 69-70, Del 144, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H), of which N501Y is in the receptor-binding domain (RBD).
Introduction: The Beta (B.1.351) variant encodes an S protein with nine mutations (L18F, D80A, D215G, Del 241-243, K417N, E484K, N501Y, D614G and
Booster of mRNA-1273 Strengthens SARS-CoV-2 Omicron Neutralization.
Introduction: A post-boost improvement in neutralization also was seen for Beta (3.4- and 2.6-fold less susceptible, respectively, than D614G).
Introduction: A second subset of COVE samples having moderate titers against D614G was pre-selected to avoid potential bias of high titer sera.
Introduction: Alpha and Delta were modest neutralization escape variants, being 2-3 fold less susceptible than D614G to neutralization by mRNA-1273 vaccine-induced antibodies and having little impact on mRNA-1273 vaccine efficacy.
Introduction: An approximate 12-fold improvement in Omicron neutralization was seen after the 50 mug boost such that the variant was now only 6.5- and 4.2-fold less neutralization-susceptible, respectively, than D614G (Fig 1).
Introduction: Both laboratories assayed D614G and Beta as
The fatty acid site is coupled to functional motifs in the SARS-CoV-2 spike protein and modulates spike allosteric behaviour.
PMID: 34934478
2021
Computational and structural biotechnology journal
Abstract: Simulations of the D614G mutant show differences in behaviour between these c
Conclusion: Simulations of the D614G spike show that this mutation affects communication between the FA site and the FPPR and the S2' cleavage site.
Conclusion: The D614G mutant shows reduced response of the FPPR and a slower rate of signal propagation to the S2' cleavage site compared to the wild-type protein (Movie 2).
Conclusion: These results indicate that the D614G mutation affects the allosteric behaviour and the response to LA of the spike, which may be related to the changes in viral fitness associated with this mutation.
Figure: Response of the wild-type and D614G spike to LA removal.
The Development of SARS-CoV-2 Variants: The Gene Makes the Disease.
PMID: 34940505
2021
Journal of developmental biology
Abstract: Some concerning mutations associated with an impact on viral fitness have been described in the Spike protein, such as D614G, N501Y, E484K, K417N/T, L452R, and P681R, among others.
Abstract: The first known mutation associated with higher transmissibility, D614G, was detected in early 2020.
Introduction: A new clade bearing mutation D614G, called A2a or Clade G, identified in February 2020, became the founder of the B1 lineage and spread globally.
Introduction: Chen and colleagues reported that sera from BNT162b2-vaccinated individuals showed decreased neutralizing potency against Alpha (2-fold), E484K +
A Conservative Replacement in the Transmembrane Domain of SARS-CoV-2 ORF7a as a Putative Risk Factor in COVID-19.
Abstract: Well-characterized mutations in the spike protein, such as D614G, N439K, Delta69-70, E484K, or N501Y, are currently defining specific variants; however, some less studied mutations outside the spike region, such as p.
Insights into the Binding of Receptor-Binding Domain (RBD) of SARS-CoV-2 Wild Type and B.1.620 Variant with hACE2 Using Molecular Docking and Simulation Approaches.
SARS_CoV_2 mutation literature information.
PMID: 
2021
PLoS computational biology
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