High-resolution melting analysis after nested PCR for the detection of SARS-CoV-2 spike protein G339D and D796Y variations.
PMID: 35349821
2022
Biochemical and biophysical research communications
Introduction: reported the detection of D614G (nucleotide mutation: A23403G) and L452R (nucleotide mutation: T22917G) variations in the SARS-CoV-2 spike protein by post-PCR HRM analysis, respectively.
Discussion: In the case of detection for the D614G variation (mutation A23403G) reported by Gazali et al., the difference in the Tm value was 0.23 C.
AstraZeneca COVID-19 vaccine induces robust broadly cross-reactive antibody responses in Malawian adults previously infected with SARS-CoV-2.
Abstract: The anti-RBD IgG antibodies from these vaccinated individuals were broadly cross-reactive against multiple VOCs and had neutralisation potency against original D614G, beta, and delta variants.
Figure: D Magnitude of neutralisation activity against the beta, D614G, and delta variants in pre- and post-vaccination sera.
Figure: E Number of individuals with neutralisation activity against Beta or D614G or Delta variants in pre- and post-vaccination sera.
Figure: Magnitude of neutralisation activity against the original variant (D614G) in first wave sera with varying concentrations of A anti-Spike IgG and B anti-RBD IgG antibodies.
Figure: The Spike and PMID: 35345417
2022
The Lancet. Microbe
Figure: (A) Neutralising antibody titres against the original SARS-CoV-2 strain from Wuhan, China (IPBCAMS-WH-01/2019, number EPI_ISL_402123), and the D614G, beta (B.1.351), and delta (B.1.617.2) variants in 141 patients.
Figure: Humoral and cellular immune responses to the original SARS-CoV-2 strain, and the D614G, beta, and delta variants, in recovered patients 12 months after infection.
Discussion: Both the D614G and the delta variants escape from the neutralising antibodies against the original strain, an effect that depends on neutralising antibody titres.
Discussion: However, higher neutralising antibody titres against the original strain contributed to the protection from infection of D614G and delta variants in vitro.
Discussion: However, the D614G variant enhances infectivity mainly
The dynamics of circulating SARS-CoV-2 lineages in Bogor and surrounding areas reflect variant shifting during the first and second waves of COVID-19 in Indonesia.
Discussion: Although not associated with worse disease severity, the S_D614G change has been implicated in enhanced transmission and higher viral loads .
Discussion: For Delta variants, we observed 12 key amino acid changes in spike protein that were mostly similar to other studies, including S_T19R, S_T95I, S_G142D, S_E156-, S_F157-, S_R158G, S_K417N, S_L452R, S_T478K, S_D614G, S_P681R, and S_D950N .
Discussion: On the other hand, substitutions in the region encoding the spike protein in Indonesian lineages mainly consisted of thr
E-Volve: understanding the impact of mutations in SARS-CoV-2 variants spike protein on antibodies and ACE2 affinity through patterns of chemical interactions at protein interfaces.
Introduction: The Beta variant has non-synonymous spike mutations such as LAL 242-244 deletion, D80A, D215G, E484K, N501Y, A701V, L18F, R246I, K417N, and D614G.
Circulation of SARS-CoV-2 Variants among Children from November 2020 to January 2022 in Trieste (Italy).
Result: Briefly, in all sequences (32/32, 100%), the D614G substitution was detected, lineage B.1.
Result: Finally, 1/32 sequence was the Omicron strain, with the following mutations in the RBD domain: T22882G (N440K), G22898A (G446S), G22992A (S477N), C22995A (T478K), A23013C (E484A), A23040G (Q493R), G23048A (G496S), A23055G (Q498R),
Neutralisation Hierarchy of SARS-CoV-2 Variants of Concern Using Standardised, Quantitative Neutralisation Assays Reveals a Correlation With Disease Severity; Towards Deciphering Protective Antibody Thresholds.
Introduction: As the pandemic progressed, a number of single amino acid mutations in the Spike protein were detected, such as D614G and A222V.
Introduction: Referred to as Cluster 5 or B.1.1.298, several different groups of mutations were identified, with the most abundant population containing missense and deletion mutations on the Spike; 69/70del, Y453F and D614G.
Introduction: The D614G mutation was found to increase the density of Spike protein on virions and infectivity.
Table: D614G
Functional analysis of polymorphisms at the S1/S2 site of SARS-CoV-2 spike protein.
3Introduction: The D614G exchange increases the percentage of S proteins present in the ""open"" conformation required for efficient ACE2 binding and viruses bearing this exchange show accelerated transmission kinetics in animal models."
Introduction: Nevertheless, mutations in SARS-CoV-2 have been detected and viruses with a D614G exchange became dominant early in the pandemic.
Discussion: Why mutant S686G showed enhanced interaction with ACE2 is at present unclear but might be due to its RBD adopting a conformation that may favour ACE2 binding, which would be a similar effect as reported for mutation D614G that is also located outside of the RBD.
Differential neutralizing antibody responses elicited by CoronaVac and BNT162b2 against SARS-CoV-2 Lambda in Chile.
Abstract: Compared with the ancestral virus, neutralization against D614G, Alpha, Gamma, Lambda and Delta variants was reduced by between 0.93- and 4.22-fold for CoronaVac, 1.04- and 2.38-fold for BNT162b2, and 1.26- and 2.67-fold for convalescent plasma.
Delta Variant with P681R Critical Mutation Revealed by Ultra-Large Atomic-Scale Ab Initio Simulation: Implications for the Fundamentals of Biomolecular Interactions.
3Introduction: In addition to the P681R mutation, the Delta variant also contains a D614G mutation, which promotes the RBD of the S-protein in an ""open"" conformation, making its binding with the ACE2 receptor easier, as well as enhancing the protease cleavage at the S1/
Abstract: Here, using ultra large-scale ab initio computational modeling, we study the P681R and D614G mutations in the SD2-FP domain, including the effect of double mutation, and compare the results with the wild type.
Abstract: Together with the earlier reported mutation D614G in the same domain, it offers an excellent instance to investigate the nature of mutations and how they affect the interatomic interactions in the spike protein.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Biochemical and biophysical research communications
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