Abstract: In addition, the neutralization efficacy of authentic SARS-CoV-2 wild type (D614G), Delta, and Omicron by sera from 2x or 3x BNT162b2-vaccinated persons was analyzed.
Result: The neutralization of authentic SARS-CoV-2 wt (with D614G mutation), Delta, and Omicron was analyzed using sera of 2x BNT162b-vaccinated and BNT162b2 boost-vaccinated individuals.
Figure: Neutralization of SARS-CoV-2 wild type (D614G), Delta, and Omicron.
Figure: Neutralization of authentic SARS-CoV-2 wt (including D614G mutation), Delta, and Omicron using sera of 2x BNT162b-vaccinated (A) and BNT162b2 boost-vaccinated (B) individuals.
Antibody evasion properties of SARS-CoV-2 Omicron sublineages.
Introduction: Among these samples, the mean serum neutralizing titres against Omicron sublineages were significantly lower than the mean titre for D614G; although the mean titre was slightly lower for BA.2, t
Introduction: a marked and significant loss of neutralizing activity of the serum against BA.1+R346K and BA.2 relative to D614G was noted, with neutralizing titres for numerous samples dropping below the limit of detection.
Method: SARS-CoV-2 variants D614G (GISAID: EPI_ISL_497840) and BA.2 (GISAID: EPI_ISL_9845731) were isolated from respiratory tract specimens of patients with COVID-19 in Hong Kong by J.F.-W.C., K.-Y.Y.
Figure: b, Fold change in IC50 values relative to D614G of neutralization of Omicron variants, as well as point mutants unique to BA.2.
Antibody escape and global spread of SARS-CoV-2 lineage A.27.
Introduction: A.27 is characterized by a mutational profile including L18F, L452R and N501Y in the S protein that combines genetic changes found in various VOCs/VOIs, while lacking the D614G substitution present in most other lineages.
Introduction: Early in the pandemic, SARS-CoV-2 acquired the S D614G substitution that has been associated with increased transmissibility and set the genetic foundation for the large number of B.1 derived lineages.
Introduction: These variants are characterized by specific patterns of concerning S mutations: apart from the D614G substitution, lineage B.1.1.7 has the N501Y amino acid substitution asso
Evolution of the SARS-CoV-2 spike protein in the human host.
Result: Our binding data on the variants and engineered constructs show that the D614G substitution is a prerequisite for the tighter receptor binding of changes in RBD, like N501Y, but do not explain how it facilitates the increase in affinity.
Result: The substitution D614G (relative to Wuhan) occurred earlier in the evolution of SARS-CoV-2, became the predominant global form of the virus and continues to be present in the Alpha and Beta variant forms of the virus.
Result: They follow D614G substitution which was acquired early in the pandemic and similarly acted to increase the spike stability.
Result: We suggest that one of effects of the more open conformation promoted by the D614G substitution is to increase the proportion o
COVID-19 outbreak in Malaysia: Decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang.
PMID: 35257681
2022
Journal of virological methods
Introduction: For the simultaneous detection of SARS-CoV-2 and its variant (D614G mutant), FAM and ROX fluorophores were attached to each probe during probe sequence design to enable target discrimination.
Introduction: In the experiment, the specificity and sensitivity of RdRp and N target primer pairs and probe sets were confirmed in the L clade, chosen to represent viruses containing the target gene without the D614G mutation.
Introduction: In this milieu, we developed a rRT-PCR detection method that simultaneously detects common SARS-CoV-2 and the SARS-CoV-2 D614 strain (a mutant with the replacement of aspartic acid with glycine at position 614 of the spike glycoprotein (S protein)), which has become a major circulating strain worldwid
Omicron variant (B.1.1.529) of SARS-CoV-2: understanding mutations in the genome, S-glycoprotein, and antibody-binding regions.
Abstract: We also evaluated mutations in the antibody-binding regions and observed some important mutations overlapping those of previous variants including N501Y, D614G, H655Y, N679K, and P681H.
Result: Mutations previously reported as important in different variants were N501Y, D614G, H655Y, N679K, and P681H.
Discussion: The significant mutations and features are N501Y (augments the binding between of S-protein and ACE2); D614G (increase infectivity); H655Y (accelerate transmission);
A structural dynamic explanation for observed escape of SARS-CoV-2 BA.2 variant mutation S371L/F.
Introduction: Altered closed versus open state occupancy also explains the expected S371L/F fitness cost, as a tradeoff between closed-state occupancy and infectivity was recently established via smFRET characterization of the altered conformational dynamics due to the D614G and Alpha variant mutations by Yang et al.
Introduction: provide a detailed analysis of loss of potency by evaluating vaccine/convalescent sera and therapeutic antibodies against pseudotyped viruses with D614G spike proteins harboring single point mutations from the variants of concern (VOCs).
Vaccine-Induced Antibody Responses against SARS-CoV-2 Variants-Of-Concern Six Months after the BNT162b2 COVID-19 mRNA Vaccination.
Abstract: Variants D614G, Alpha, and Eta are neutralized by sera of 100% of vaccinees, whereas neutralization of Delta is 3.8-fold reduced and neutralization of Beta is 5.8-fold reduced compared to D614G.
Introduction: We also analyzed the neutralization capacity of vaccinees' sera on two ancestral strains (Wuhan-like and D614G), three VOCs (Alpha, Beta, and Delta), and one VBM (Eta).
Result: At 3 months after the vaccination, all vaccinees still had neutralizing antibodies against D614G, Alpha, and Eta variants, while Beta was neutralized by 84.6% (44/52) and Delta by 96.2% (50/52) of vaccinees' sera (Table 1).
Result: des
Discussion: We observed similar neutralization titers against D614G, Alpha, and Eta, whereas neutralization of Beta and Delta was reduced or lost 6 months postvaccination.
Characterization of SARS-CoV-2 Variants B.1.617.1 (Kappa), B.1.617.2 (Delta), and B.1.618 by Cell Entry and Immune Evasion.
Introduction: For example, the D614G mutation, identified during the earlier stage of the pandemic, promotes spike binding to ACE2, leading to enhanced virus transmission.
Method: The desired mutations in the spike proteins of Kappa, Delta, and B.1.618 variants in an SARS-CoV-2 isolate, Wuhan-Hu-1, with a D614G (WT) backbone and the trVLP, were generated as previously described.
Result: To further examine the biological impact of these mutations on cell entry, we produced pseudotyped virus
Figure: Luciferase activity was determined after 2 days of infection, and data were normalized to the WT (D614G) of the individual experiment.
Figure: This experiment was repeated twice independently, and data were normalized to the WT (D614G) of the individual experiment.
Tetra-primer ARMS-PCR combined with dual-color fluorescent lateral flow assay for the discrimination of SARS-CoV-2 and its mutations with a handheld wireless reader.
Abstract: Herein, we report a low-cost, facile, and highly sensitive diagnostic platform that can simultaneously distinguish wild-type (WT) SARS-CoV-2 and its two mutations, namely, D614G and N501Y, within 2 h.
Abstract: The WT and M viruses were indicated and were strictly discriminated by the presence of a green or red band on test line 1 for the D614G site and test line 2 for the N501Y site.
Abstract: The limits of detection (LODs) for the WT and M D614G were estimated as 78.91 and 33.53 copies per muL, respectively.
SARS_CoV_2 mutation literature information.
PMID: 
2022
BMC medicine
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