Method: Pymol mutagenesis wizard was used to model Q675H and D614G for both wild-type (wt) and mutant systems.
Result: In particular, in B.1.438.1, B.1.438.2, B.1.438.3, and B.1.1.385 lineages, Q675H and D614G are the sole mutations located in the spike protein.
Prediction of SARS-CoV-2 Variant Lineages Using the S1-Encoding Region Sequence Obtained by PacBio Single-Molecule Real-Time Sequencing.
Abstract: Seven compounds reduced weight loss and promoted weight regain of hamsters infected not only with the original strain but also the D614G variant.
A Potent and Protective Human Neutralizing Antibody Against SARS-CoV-2 Variants.
Method: The fold change of the mutant spike relative to WT D614G in binding or neutralization was calculated by simple division of the respective IC50 or MFI values.
Figure: (C-E) The relative mean fluorescence intensity (MFI) of mAbs or ACE2 binding was determined by comparing the total MFI in the selected gate between the spike variants and WT D614G.
Figure: The IC50 of antibodies against WT D614G are listed in (A).
Figure: Values indicate the fold changes in half-maximal inhibitory concentrations (IC50) (A) and the mean fluorescence intensity (MFI) relative to that of wild-type (WT) D614G (B).
SARS-CoV-2 Variants: Mutations and Effective Changes.
PMID: 34975266
2021
Biotechnology and bioprocess engineering
Abstract: Certain mutations (D614G, E484K, N501Y, K417N, L452R and P681R) have appeared across several different strains, often accompanied by others that may be complementary working together to confer increased infectivity, fitness, or resistance to neutralization.
SARS-CoV-2 Omicron neutralization by therapeutic antibodies, convalescent sera, and post-mRNA vaccine booster.
Abstract: A booster vaccination significantly increased titers against Omicron to levels comparable to those seen against the ancestral (D614G) variant after two immunizations.
Introduction: We compared the magnitude of neutralization escape by Omicron to D614G (referred to as wild type, WT) and Delta SARS-CoV-2 variants to help inform public health decisions and offer further data toward correlate of protection research.
Discussion: Here, using the same lentiviral pseudovirus neutralization platform we measured the change in potency of 24 clinical-stage therapeutic antibodies against Omicron compared to WT (D614G) and compared neutralizing antibodies in sera from two well-characterized cohorts of subjects in prospective clinical studies.
Emergence of Drift Variants That May Affect COVID-19 Vaccine Development and Antibody Treatment.
Conclusion: The highly prevalent 23403A>G (p.D614G) variant in the European population ma
Discussion: A recent report corroborated our findings of high prevalence of D614G in Europe.
Discussion: However, given the small sample size, it is hard to ascertain whether D614G is the dominant strain in these countries.
Discussion: In addition to the Netherlands, Switzerland, and France, our data indicate that the D614G sub-strain is frequently detected in Brazil, Finland, and Belgium.
Discussion: Intriguingly, in our data the D614G variant was detected only in 2 out of 151 Chinese patients analyzed.
Discussion: Notably, these two samples do not share common variants besides p.D614G.
A Novel Synonymous Mutation of SARS-CoV-2: Is This Possible to Affect Their Antigenicity and Immunogenicity?
Abstract: These two subtypes were divided by a novel synonymous mutation of D614G.
Introduction: If the mutation of D614G plays a crucial role in the positive selection process, SARS-CoV-2b will be the dominant type of SARS-CoV-2 in the future.
Introduction: Since no amino acid changes were found in this area other than the change of D614G, it is believed that this amino acid change alters the conformation of these immunogenic determinants; consequently, this region is expected to no longer act as a B-cell epitope in SARS-CoV-2b.
Introduction: The results of the antigenic index analysis showed severely reduced indexes of amino acids 615-617 in the SARS-CoV-2b strains compared to SARS-CoV-2a; it is predicted that the change of D614G affects the antigenicity of this region (Figure 1F).
Molecular Detection of SARS-CoV-2 Infection in FFPE Samples and Histopathologic Findings in Fatal SARS-CoV-2 Cases.
PMID: 32451533
2020
American journal of clinical pathology
Table: D614G
Could the D614G substitution in the SARS-CoV-2 spike (S) protein be associated with higher COVID-19 mortality?
PMID: 32464271
2020
International journal of infectious diseases
Introduction: For example, a substantial number of strains with the S-D614G variant are from the countries Belgium, Spain, Italy, France, Netherlands, and Switzerland that top the death toll (Figure 1B) (https://www.worldometers.info/coronavirus/; last accessed May 2, 2020); while Germany and Kuwait, with a lower death toll, constitute most strains with the wild-type 614D at S (Figure 1B).
Introduction: Overall, our observation speculates that the S-D614G strains may be more
Figure: The mutation observed at the S protein of the SARS-COV-2, D614G in white color, may create conformational changes mimicking the open status and facilitate the cleavage domain's exposure to proteases FURIN or TMPRSS2 and could be sufficient to speed up the cleavage.
SARS_CoV_2 mutation literature information.
PMID: 
2021
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