Abstract: It is evident that the D614G mutation in spike glycoprotein and P4715L in RdRp is the important determinant of SARS-CoV-2 evolution since its emergence.
Discussion: D614G mutation in spike is the dominant pandemic form that may indicate a fitness advantage and related to severe reduced antigenic specificity.
Discussion: However, careful examination of the cryoEM structure predicts that the interactions with the neighbouring protomer due to D614G mutation is disrupted in absence of negatively charged/hydrogen bond accepting group from the side chain of non-polar glycine.
Discussion: However, it can be anticipated that the replacement of negatively charged Asp into non-polar Gly in D614G<
D614G mutation alters SARS-CoV-2 spike conformational dynamics and protease cleavage susceptibility at the S1/S2 junction.
Abstract: Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide.
Abstract: Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.
Decline of Humoral Responses against SARS-CoV-2 Spike in Convalescent Individuals.
Abstract: Similarly, we observed a significant decrease in the capacity of convalescent plasma to neutralize pseudoparticles bearing wild-type SARS-CoV-2 S or its D614G variant.
Introduction
Figure: (A) Pseudoviral particles coding for the luciferase reporter gene and bearing SARS-CoV-2 S glycoprotein or its D614G counterpart, SARS-CoV S glycoprotein, or VSV-G glycoprotein were used to infect 293T-ACE2 cells.
Figure: (D to F) Cell surface staining of 293T cells expressing full-length Spike (S) from different HCoVs, including (D) SARS-CoV-2 or its D614G counterpart; (E) SARS-CoV; and (F) OC43, NL63, and 229E with plasma samples recovered at baseline (6 weeks after the onset of symptoms) and 1 month later.
Geographical Distribution of Genetic Variants and Lineages of SARS-CoV-2 in Chile.
Abstract: Complete genome analysis of 141 viral samples from different regions of Chile revealed a predominance of variant D614G like in Europe and the USA and the major presence of lineage B.1.
Introduction: The genetic variants are located in the nucleotide positions 23,403, 26,144, and 28,144 based on the reference sequence NC_045512.2, and the variant's name is represented by a capital letter that corresponds to the amino acid substitution product of the SNP G: Spike:D614G, V: NS3:G251V, S: ORF8:L84S, respectively, and O for other strains that keep some of the nucleotide as the reference strain on that genome position that cannot be assigned to the previous described clades.
Therapeutically Targeted Destabilization of the Quaternary Structure of the Spike Protein in the Dominant G614 Strain of SARS-CoV-2.
Abstract: The 'D614G' mutation (Aspartate-to-Glycine change at position 614) of the SARS-CoV-2 spike protein has been speculated to adversely affect the efficacy of most vaccines and countermeasures that target this glycoprotein, necessitating frequent vaccine matching.
Abstract: We additionally demonstrate that the putative elastase cleavage site introduced
Introduction: This mutation has resulted in a number of articles and preprints postulating that isolates containing this 'D614G' mutation have a structural advantage, including as a better substrate to the S1 furin cleavage domain, and are associated with an increase in: (a) transmissibility and viral loads; (b) transduction of human cells; (c) pathogenicity and case fatality.
Figure: A D614G mutation is expected to disrupt both these interactions.
Structural impact on SARS-CoV-2 spike protein by D614G substitution.
Abstract: Substitution for aspartic acid by glycine at position 614 in the spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the ongoing pandemic, appears to facilitate rapid viral spread.
Coding-Complete Genome Sequences of 23 SARS-CoV-2 Samples from the Philippines.
Abstract: Mutation analysis showed the presence of the D614G mutation in the spike protein in 22 of 23 genomes.
Introduction: Four amino acid substitutions (ORF1b-P314L, S-D614G, N-R203K, and N-G204R) unique to clade GR (both lineages B.1.1 and B.1.1.28) were seen in all our sequences in this clade.
Introduction: From the same set of data, lineage B.1.1 containing D614G was found in a sample collected on 25 June 2020.
Introduction: None of the Philippine SARS-CoV-2 sequences in GISAID (accessed 22 August 2020) with a collection date before 25 June 2020 contained the
1Abstract: The SARS-CoV-2 does not appear to present a mutational ""hot spot"" as only the D614G mutation has been identified from clinical isolates."
3Introduction: Although human Coronaviruses frequently contain ""hot spots"" for non-synonymous amino acid replacements affecting host tropism/adaptation, resistance to neutralizing antibodies and immune evasion, only a single high-frequency non-synonymous mutation (D614G) has been identified from clinical SARS-CoV-2 isolates."
Introduction: Furthermore, it has been demonstrated that the D614G mutation in the SARS-CoV-2 S protein reduced S1 shedding and increased infectivity.
COVID-19 neutralizing antibodies predict disease severity and survival.
Abstract: In addition to neutralization of wild-type SARS-CoV-2, patient sera were also able to neutralize the recently emerged SARS-CoV-2 mutant D614G, suggesting protection from reinfection by this strain.
Introduction: One mutation, D614G, has rapidly become the predominant transmitted variant by outcompeting wildtype infections.
Result: Given the importance of humoral immunity in preventing most viral infections, the recent emergence of a mutation in the SARS-CoV-2 spike protein (D614G) has raised concerns for the potential for convalescent patients to become re-infected.
Result: To det
Discussion: We found that one such mutation, D614G, which has now spread and become a dominant strain worldwide, does not affect the neutralizing ability of patient sera, reducing concerns for re-infection.
SARS_CoV_2 mutation literature information.
PMID: 
2020
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