Abstract: Here we show that changes in the inter-protomer energetics due to the D614G substitution favor a higher population of infection-capable (open) states.
Abstract: The COVID-19 pandemic underwent a rapid transition with the emergence of a SARS-CoV-2 variant that carried the amino acid substitution D614G in the Spike protein that became globally prevalent.
SARS-CoV-2 mRNA vaccine design enabled by prototype pathogen preparedness.
Abstract: Here we show that mRNA-1273 induces potent neutralizing antibody responses to both wild-type (D614) and D614G mutant2 SARS-CoV-2 as well as CD8+ T cell responses, and protects against SARS-CoV-2 infection in the lungs and noses of mice without evidence of immunopathology.
Introduction: Pseudovirus neutralizing activity in 1 mug mRNA-1273-immunized mice was si
Figure: Comparisons between D614 and D614G were made by two-sided Mann-Whitney test within each study, and no significance was detected.
Figure: Sera were collected 2 weeks post-boost and assessed for neutralizing antibodies against homotypic SARS-CoV-2_D614 pseudovirus (filled circles) or SARS-CoV-2_D614G (unfilled circles).
Figure: mRNA-1273 elicits robust pseudovirus neutralizing antibody responses to SARS-CoV-2_D614G.
Genome Sequences of Six SARS-CoV-2 Strains Isolated in Morocco, Obtained Using Oxford Nanopore MinION Technology.
Abstract: Mutation analysis revealed the presence of the spike D614G mutation in all six genomes, which is widely present in several genomes around the world.
Introduction: All the genomes shared four mutations, namely, two synonymous (F924F and L4715L), one nonsynonymous (D614G), and one intergenic (241C>T).
Introduction: Only one nonsynonymous mutation was detected (D614G) in the spike protein, which is known as the most prevalent variant worldwide, and it is also associated with the emergence of clade A2, which includes all Moroccan strains sequenced in this study.
Comprehensive Analyses of SARS-CoV-2 Transmission in a Public Health Virology Laboratory.
Abstract: Complete SARS-CoV-2 genomes were sequenced and mutation analyses showed inclusion of all samples to clades 20B and 20C, possessing the spike mutation D614G.
Introduction: For example, using Nextstrain's nomenclature, there are currently five major clades: 19A (the root clade) and 19B, and clades 20A, B and C that are widespread in Europe and include a mutation in the spike protein, D614G, that is associated with increased infectivity and higher viral loads.
Discussion: Depending upon whether these mutations are beneficial for the virus, they may survive and be transmitted over time, for example, as occurred with the emerging clade 20-associated mutation A23403G/D614G that changed aspartate (acidic group) to glycine (aliphatic group) in the COVID-19 outbreak in Malaysia: Decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang.
PMID: 32779780
2020
Journal of computational chemistry
Abstract: D614G spike glycoprotein (sgp) mutation in rapidly spreading severe acute respiratory syndrome coronavirus-2 (SARS-COV-2) is associated with enhanced fitness and higher
Introduction: A major finding from a recent study is guanine-to-adenine transition in position 23,403 in the Wuhan reference strain leading to aspartate-to-glycine mutation in spike glycoprotein residue 614 (D614G).
Introduction: Notably, at 75 ns, the G614-T859 inter-residue distance further increased to ~2.5 nm and was maintained till about 135 ns but moved back to its initial state of 1.0 nm at the end of the simulation; thus, providing the first evidence in support of S1 shedding from viral-membrane-bound S2 following D614G mutation (Figure 1a).
Geographic and Genomic Distribution of SARS-CoV-2 Mutations.
Abstract: In particular, clade G, prevalent in Europe, carries a D614G mutation in the Spike protein, which is responsible for the initial interaction of the virus with the host human cell.
Introduction: According to data from the public database of the Global Initiative on Sharing All Influenza Data (GISAID), three major clades of SARS-CoV-2 can be identified (Forster et al.,), that have been subsequently named as clade G (variant of the spike protein S-D614G), clade V (variant of the ORF3a coding protein NS3-G251), and clade S (variant ORF8-L84S).
Table: D614G
Discussion: The prevalent
Characterizing SARS-CoV-2 mutations in the United States.
Result: Among them, 3 mutations are synonymous ones (i.e., 3037C>T-(F106F), 8782C>T-(S76S), and 18060C>T-(L7L)) and 8 mutations are the missense mutations (i.e., 14408C>T-(P323L), 23403A>G-(D614G), 25563G>T-(Q57H), 1059C>T-(T85I), 28144T>C-(L84S), 17858A>G-(Y541C), 17747C>T-(P504L), and
COVID-19 outbreak in Malaysia: Decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang.
Introduction: 3B), but further analyses would be required to assess whether the D614G mutation has an effect on the way the S protein changes its conformation after interaction with ACE2.
Introduction: A comparison against the previous set of genomes collected for our phylogenetic and molecular dating analysis revealed that for samples submitted during the period from March 17-30, 2020, the D614G clade became increasingly prevalent worldwide, expanding from 22 to 42 countries.
Introduction: Accordingly, it was not possible to draw any conclusions regarding the clinical phenotype of the D614G clade and there is no evidence at this time to suggest this clade is associated with any differences in disease phenotype.
Introduction: Although the mutation appears to be clade-specific, we noted that D614G also
Molecular Epidemiology Analysis of SARS-CoV-2 Strains Circulating in Romania during the First Months of the Pandemic.
Result: These mutations, C3037T, C14408T (P323L in Nsp12) and A23403G (D614G in S), were present in all Romanian sequences.
Result: When looking into the GISAID database mutational statistics, which uses hCoV-19/Wuhan/WIV04/2019 EPI_ISL_402124 as a reference strain (COVsurver tool), both P323L and D614G mutations are present in a high percentage (61.7%/61.8%, n = 33480 and n = 33537).
Discussion: A subsequent study reported that A23403G (D614G in S) is becoming prevalent worldwide and associated with severe disease cases.
miRNA target prediction might explain the reduced transmission of SARS-CoV-2 in Jordan, Middle East.
Abstract: The exciting findings here that the nucleotide substitution 1841A > G at the viral genomic RNA level, which is an amino acid substation D614G at the spike protein level showed a change in the predicted miRNA sequence from hsa-miR-4793-5p to hsa-miR-3620-3p with an increase in the target score from 91 to 92.
Result: The interesting finding here that the 1841A > G, and D614G showed a change in the predicted miRNA and an increase in the target score from 91 to 92 (hsa-miR-4793-5p to hsa-miR-3620-3p).
SARS_CoV_2 mutation literature information.
PMID: 
2020
bioRxiv
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