Result: D614G original virus can be well neutralized by D614G, VOCs (Alpha, Beta, Gamma, Delta), and VOIs (Lambda, Mu) spike protein immunized sera, with NT50 values of 12,130, 9238, 4871, 3535,
Result: Among them, the D614G immunized serum had the strongest neutralization protection to the original D614G strain.
Result: Besides, the sera immunized with spike protein from D614G, Alpha, Delta, and Mu variants are more protective against D614G, B.1.640.1, and B.1.630 variants.
Result: Cluster analysis showed that D614G, B.1.640.1, and B.1.630 showed similar antigenicity to different immunogens (Figure 3C).
Table: D614G
Characteristic analysis of Omicron-included SARS-CoV-2 variants of concern.
Introduction: And compared to the original strain of SARS-CoV-2, the D614G mutation makes the S protein more stable and more flexible, which makes the virus more infectious.
Introduction: For example, S protein of the D614G variants has a looser and wider trimer structure of RBD.
Introduction: In July 2020, it was reported that the strain with the spike protein D614G mutation in Europe is more contagious and may become the main form of the virus pandemic.
Result: In addition, Alpha, Beta, Delta, and Omicron strains had partial protruding structures at the junction of S protein and virus particles (Figure 1), which may be caused by the internal extrusion of the S p
Durability and Cross-Reactivity of SARS-CoV-2 mRNA Vaccine in Adolescent Children.
1Abstract: We tested the durability and cross-reactivity of anti-SARS-CoV-2 serologic responses over a six-month time course in vaccinated adolescents agains
3Introduction: Here, we quantified relative antibody responses in adolescent children immediately following the Pfizer-BioNTech mRNA vaccination and six months post-inoculation and analyzed the efficacy of the humoral response against the D614G (""wild type"") SARS-CoV-2 and latest variant of concern (VOC), Omicron."
4Method: Serological analyses were performed using an in-house enzyme-linked immunosorbent assay (ELISA) that detects IgG against the D614G (""wild type"") SARS-CoV-2 Spike, the D614G (""wild type"") Receptor-Binding Domain (RBD), or the Omicron SARS-CoV-2 VOC RBD by using the previously described method."
Safety and Immunogenicity of Inactivated COVID-19 Vaccines Among People Living with HIV in China.
Discussion: However, the GMT for the delta variant was significantly lower than the GMT for the D614G variant in PLWH, which is consistent with Chang Liu's finding that mRNA vaccines induced an antibody response to some variants, but the neutralization of the delta variant was reduced.
Discussion: SARS-CoV-2 IgG concentrations and neutralizing antibody titers against the D614G and delta variants also declined significantly in PLWH compared to HDs.
Discussion: The D614G and delta variants harbor mutations in the RBD, so neutralizing titers to the pseudotyped virus may better show the immune response to the two variants elicited by vaccination than titers of S-RBD-IgG.
Discussion: The positive rates of neut
Delta variant (B.1.617.2) of SARS-CoV-2: Mutations, impact, challenges and possible solutions.
PMID: 35507895
2022
Human vaccines & immunotherapeutics
Abstract: The enhanced transmissibility of Delta variant has been associated with critical mutations such as D614G, L452R, P681R, and T478K in the S-protein.
Whole genome sequencing of SARS-CoV2 strains circulating in Iran during five waves of pandemic.
Abstract: There were different mutations in all parts of the genomes but Spike-D614G, NSP12-P323L, N-R203K and N-G204R were the most frequent mutants in these studied viruses.
The twin-beginnings of COVID-19 in Asia and Europe-one prevails quickly.
Abstract: The first wave is a group of four mutations (C241T, C3037T, C14408T and A23403G [this being the amino acid change D614G]; all designated 0 to 1 below).
Abstract: This DG (D614G) group, fixed at the start of the pandemic, is the foundation of all subsequent waves of strains.
RBD-mRNA vaccine induces broadly neutralizing antibodies against Omicron and multiple other variants and protects mice from SARS-CoV-2 challenge.
Abstract: The vaccine induced durable antibodies that potently neutralized prototypic strain and B.1.1.7 lineage variant pseudoviruses containing N501Y or D614G mutations alone or in combination with a N439K mutation (B.1.258 lineage), with a L452R mutation (B.1.427 or B.1.429 lineage), or a L452R-E484Q double mutation (B.1.617.1 variant), although neutralizing activity against B.1.1.7 lineage variant containing 10 amino acid changes in the S protein was slightly reduced.
Biomechanical Dependence of SARS-CoV-2 Infections.
Abstract: Given the recent data highlighting the importance of alternative virulent strains, we included both the native strain identified in early 2020 and an early S protein variant (D614G) that was shown to increase the viral infectivity markedly.
Abstract: Our results show that cells on softer and sparser scaffolds, closer resembling younger lungs, exhibit higher infection rates by the WT and D614G variant.
Functional Analysis of Spike from SARS-CoV-2 Variants Reveals the Role of Distinct Mutations in Neutralization Potential and Viral Infectivity.
Introduction: This process is driven by both escape from neutralizing antibodies, where mutations like E484K, E484Q, S477N play key rol
Result: Initially, single point FCS mutations were inserted into the Wuhan wild-type (D614G) spike.
Discussion: These reports demonstrate that spike residues like D614G and N501Y are located at the distal region of the spike RBD and facilitate transitions from a closed to open state of the spike prior to ACE2 binding, enhancing the stability and affinity of the viral spike to its receptor and subsequently affecting viral spread.
SARS_CoV_2 mutation literature information.
PMID: 
2022
MedComm
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