Abstract: Simultaneously we have discussed the significant mutations related to emerging variants and immune escape, such as mutations in the RBD region (N439K, L452R, E484K, N501Y, K444R) and other parts (D614G, P681R) of the S-glycoprotein.
Introduction: Another mutation in the S-glycoprotein region is D614G, which is associated with immune escape.
Introduction: Few mutations include K417T/N, E484K, L452R,
Table: D614G
Rapid detection of the widely circulating B.1.617.2 (Delta) SARS-CoV-2 variant.
Introduction: Omicron Spike was recognized less efficiently than D614G, Alpha, Beta, Gamma, and Delta Spikes.
Introduction: Plasma from vaccinated previously infected individuals recognized more efficiently all tested Spikes (D614G, Alpha, Beta, Gamma, Delta, and Omicron) than those from naive vaccinated individuals.
Method: The plasmids encoding the SARS-CoV-2 Spike variants; D614G, B.1.1.7, B.1.351, P.1 and B.1.617.2 were previously described.
Method: To produce SARS-CoV-2 pseudoviruses, 293T cells were transfected with the lentiviral vector pNL4.3 R-E- Luc (NIH AIDS Reagent Program) and a plasmid encoding for the indicated S glycoprotein (D614G, Alpha, Beta, Gam
Characterization of the First SARS-CoV-2 Isolates from Aotearoa New Zealand as Part of a Rapid Response to the COVID-19 Pandemic.
Result: All seven SARS-CoV-2 isolates showed similar growth kinetic profiles in Vero cells, with no significant difference in the viral replication slope, regardless of the presence or absence of the D614G amino acid substitution in the spike gene (Figure 7A).
Result: Interestingly, although the SARS-CoV-2 spike neutralizing mAb was able to block the replication of all seven SARS-CoV-2 isolates, viruses carrying the D614G mutation in the spike gene (NZ3, NZ4, NZ5, and NZ7) were 2.4-fold more susceptible to the mAb than the wild-type viruses (median EC50 of 0.07 and 0.17 microg/mL, p < 0.0001, respectively; Figure 8).
Result: Perhaps the most relevant amino acid substitution, i.e., the D614G in the spike gene, was observed in the sequence of fo
Ferristatin II Efficiently Inhibits SARS-CoV-2 Replication in Vero Cells.
Result: Due to the significant differences in the RBD proteins of the Wuhan D614G and the Omicron SARS-CoV-2 variants, the latter could not be detected in cell-based ELISA using the available anti-RBD antibody, as it was raised to the RBD of original SARS-CoV-2 strain.
Result: The detection of viral antigen in cell-base ELISA found that the IC50 values for ferristatin II were 26.5 and 40.4 microM for the Wuhan D614G and Delta viruses, respectively (Figure 2A).
Result: The quantification of the virus titers in culture media confirmed that the release of the virus from the infected cells into the culture medium was inhibited by ferristatin II, starting from 25 microM concentration, and IC50 values calculated from the reduction in TCID50 titers were 24.0 and 25.2 microM for the Wuhan PMID: 35215919
2022
Viruses
5Result: This strain represents an isolate from the UK's ""first wave"" and is a representative of clade B that contains the D614G mutation in Spike (Table S1)."
Result: PHE is from clade A and does not contain the D614G substitution in Spike (Supplementary Table S1).
Result: These changes included, but were not limited to, D614G in Spike; R203K and G204K in N; and an out-of-frame deletion of five nucleotides in ORF7A, leading to its premature truncation.
Discussion: Investigation of the patterns of SARS-CoV-2 genetic diversity worldwide during outbreaks has already facilitated a genetic-based nomenclature of lineages and has
In-silico genomic landscape characterization and evolution of SARS-CoV-2 variants isolated in India shows significant drift with high frequency of mutations.
PMID: 35233173
2022
Saudi journal of biological sciences
Abstract: The most frequent non-synonymous mutation 486/546 (89.01%) occurred in the S gene (structural gene) at position 23,403 where A changed to G leading to the replacement of aspartic acid by glycine in position (D614G).
Result: The most frequent non-synonymous mutation 486/546 (89.01%) occurred in the S gene at position 23,403 where A changed to G leading to the replacement of aspartic acid by glycine in position (D614G)) Table 3 and.
Table: D614G
Discussion: A23403G (D614G) was observed in West European states in the early stage of the disease and this finding might point towards the source of infection from which it was introduced in India.
Discussion: Moreover, the present study showed that
Influenza A(H1N1)pdm09 Virus but Not Respiratory Syncytial Virus Interferes with SARS-CoV-2 Replication during Sequential Infections in Human Nasal Epithelial Cells.
Abstract: We have evaluated the interactions between SARS-CoV-2 (D614G mutant) and influenza A(H1N1)pdm09 or respiratory syncytial virus (RSV) in the nasal human airway epithelium (HAE) infected simultaneously or sequentially (24 h apart) with virus combinations.
Method: SARS-CoV-2 RNA was sequenced by MinION technology (Oxford Nanopore technologies, Oxford, UK), and the D614G substitution was detected in the spike protein.
Rapid SARS-CoV-2 Intra-Host and Within-Household Emergence of Novel Haplotypes.
Introduction: The first mutation known to improve viral fitness was Spike D614G, which was first identified in the Alpha variant and then reached a prevalence of nearly 100% globally.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Frontiers in immunology
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