Abstract: Moreover, we provide experimental evidence of the broad applicability of this assay through the multiplex detection of SARS-CoV-2 variants (D614G mutation) and direct detection of bacterial 16S rRNA.
Introduction: D614G), conferring greater infectivity and more rapid spread, have become predominant in various regions.
Introduction: Moreover, STAR was utilized for multiplex detection of the D614G mutation and N gene of SARS-CoV-2 in a single tube as well as for the direct detection of bacterial 16S rRNA without additional nucleic acid purification, thereby confirming the wide applicability of this method for nucleic acid biomarker detection.
Figure: (A) Illustration of the STAR assay for the D614G mutation in the S gene of SARS-CoV-2.
Figure: (C) Lim
Durability and Cross-Reactivity of SARS-CoV-2 mRNA Vaccine in Adolescent Children.
1Abstract: We tested the durability and cross-reactivity of anti-SARS-CoV-2 serologic responses over a six-month time course in vaccinated adolescents agains
3Introduction: Here, we quantified relative antibody responses in adolescent children immediately following the Pfizer-BioNTech mRNA vaccination and six months post-inoculation and analyzed the efficacy of the humoral response against the D614G (""wild type"") SARS-CoV-2 and latest variant of concern (VOC), Omicron."
4Method: Serological analyses were performed using an in-house enzyme-linked immunosorbent assay (ELISA) that detects IgG against the D614G (""wild type"") SARS-CoV-2 Spike, the D614G (""wild type"") Receptor-Binding Domain (RBD), or the Omicron SARS-CoV-2 VOC RBD by using the previously described method."
Characteristic analysis of Omicron-included SARS-CoV-2 variants of concern.
Introduction: And compared to the original strain of SARS-CoV-2, the D614G mutation makes the S protein more stable and more flexible, which makes the virus more infectious.
Introduction: For example, S protein of the D614G variants has a looser and wider trimer structure of RBD.
Introduction: In July 2020, it was reported that the strain with the spike protein D614G mutation in Europe is more contagious and may become the main form of the virus pandemic.
Result: In addition, Alpha, Beta, Delta, and Omicron strains had partial protruding structures at the junction of S protein and virus particles (Figure 1), which may be caused by the internal extrusion of the S p
Antigenicity comparison of SARS-CoV-2 Omicron sublineages with other variants contained multiple mutations in RBD.
Result: D614G original virus can be well neutralized by D614G, VOCs (Alpha, Beta, Gamma, Delta), and VOIs (Lambda, Mu) spike protein immunized sera, with NT50 values of 12,130, 9238, 4871, 3535,
Result: Among them, the D614G immunized serum had the strongest neutralization protection to the original D614G strain.
Result: Besides, the sera immunized with spike protein from D614G, Alpha, Delta, and Mu variants are more protective against D614G, B.1.640.1, and B.1.630 variants.
Result: Cluster analysis showed that D614G, B.1.640.1, and B.1.630 showed similar antigenicity to different immunogens (Figure 3C).
Table: D614G
Role of the Microbiome in the Pathogenesis of COVID-19.
PMID: 35433495
2022
Frontiers in cellular and infection microbiology
Abstract: Soon after the first outbreak due to the wild-type strain in December 2019, a genetic variant D614G emerged in late January to early February 2020 and became the dominant genotype worldwide.
Conclusion: The two most commonly occurring mutations, Spike_D614G and Nsp12_P314L, were structurally modeled, which showed that these mutations had the potential to enhance viral entry and replication, respectively.
Introduction: Among these, the D614G clade was the most common and was first found in late January 2020 in China, according to a study conducted by.
Introduction: Earliest samples from the USA appeared to have been derived from China and belonged to basal or L84S
Table: D614G
Prolonged shedding of infectious viruses with haplotype switches of SARS-CoV-2 in an immunocompromised patient.
PMID: 35430092
2022
Journal of infection and chemotherapy
Conclusion: When haplotype 1 was compared with Wuhan-Hu-1/2019 (GenBank accession number; MN908947), it had ten non-synonymous (ORF1a:Q2702H and S2981F, ORF1b:P314L, T1404 M, P1567L, and R2684I, S gene:D614G, N gene:R203K, G204R, and M234I) and five synonymous mutations.
Comparative genomics, evolutionary epidemiology, and RBD-hACE2 receptor binding pattern in B.1.1.7 (Alpha) and B.1.617.2 (Delta) related to their pandemic response in UK and India.
PMID: 35427787
2022
Infection, genetics and evolution
Result: Again, like the previous point AA mutation, we also evaluated the D614G mutation, which was identified as the B.1.1.7 variant.
Result: In the D614G structure, the amino acid changed from Asp614 Gly.
Result: The structural evaluation of the D614G mutation was performed, showing different forms of interactions.
Result: The structural landscape of mutation analysis revealed significant mutations, such as N501Y, D614G L452R, E484Q, and P681R, which are frequently found in these two variants.
Result: The structural landscape of significant mutations (N501Y, D614G
Pathogenicity of SARS-CoV-2 Omicron (R346K) variant in Syrian hamsters and its cross-neutralization with different variants of concern.
Method: On deep sequencing following amino acid changes were found in the isolate.(NSP5_P132H,Spike_T95I,Spike_K417N,Spike_S373P,Spike_Q493R,Spike_N969K, Spike_H655Y,Spike_N856K,N_R203K,Spike_S371L,NSP3_A1892T, PMID: 35401825
2022
Theranostics
3Introduction: For example, the D614G variant, which was first identified in July 2020, has a faster infection rate and higher viral load in the upper respiratory tract than the wild-type ""Wuhan-Hu-1"" strain."
Abstract: In addition, similar results were obtained using a SARS-CoV-2 pseudovirus neutralization assay specific for wild-type S and five prevalent S variants (D614G, B.1.1.7, B.1.351, P.1, B.1.617.2), thus demonstrating that high antibody diversity is associated with high NAb titers.
Introduction: The B.1.1.7 variant (D614G, N501Y) is more infectious and may lead to increased mortality compared to the parental strain.
Result: Consistent with the observation that the trimerized form of the D614G PMID: 35403837
2022
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Abstract: Pseudotyped and authentic virus-based assays show that COVID-HIG displays broad-spectrum neutralization effects on a wide variety of SARS-CoV-2 variants, including D614G, Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Kappa (B.1.617.1), Delta (B.1.617.2), and Omicron (B.1.1.529) in vitro.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Biosensors & bioelectronics
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