Introduction: For example, the D614G mutation, identified during the earlier stage of the pandemic, promotes spike binding to ACE2, leading to enhanced virus transmission.
Method: The desired mutations in the spike proteins of Kappa, Delta, and B.1.618 variants in an SARS-CoV-2 isolate, Wuhan-Hu-1, with a D614G (WT) backbone and the trVLP, were generated as previously described.
Result: To further examine the biological impact of these mutations on cell entry, we produced pseudotyped virus
Figure: Luciferase activity was determined after 2 days of infection, and data were normalized to the WT (D614G) of the individual experiment.
Figure: This experiment was repeated twice independently, and data were normalized to the WT (D614G) of the individual experiment.
Vaccine-Induced Antibody Responses against SARS-CoV-2 Variants-Of-Concern Six Months after the BNT162b2 COVID-19 mRNA Vaccination.
Abstract: Variants D614G, Alpha, and Eta are neutralized by sera of 100% of vaccinees, whereas neutralization of Delta is 3.8-fold reduced and neutralization of Beta is 5.8-fold reduced compared to D614G.
Introduction: We also analyzed the neutralization capacity of vaccinees' sera on two ancestral strains (Wuhan-like and D614G), three VOCs (Alpha, Beta, and Delta), and one VBM (Eta).
Result: At 3 months after the vaccination, all vaccinees still had neutralizing antibodies against D614G, Alpha, and Eta variants, while Beta was neutralized by 84.6% (44/52) and Delta by 96.2% (50/52) of vaccinees' sera (Table 1).
Result: des
Discussion: We observed similar neutralization titers against D614G, Alpha, and Eta, whereas neutralization of Beta and Delta was reduced or lost 6 months postvaccination.
A structural dynamic explanation for observed escape of SARS-CoV-2 BA.2 variant mutation S371L/F.
Introduction: Altered closed versus open state occupancy also explains the expected S371L/F fitness cost, as a tradeoff between closed-state occupancy and infectivity was recently established via smFRET characterization of the altered conformational dynamics due to the D614G and Alpha variant mutations by Yang et al.
Introduction: provide a detailed analysis of loss of potency by evaluating vaccine/convalescent sera and therapeutic antibodies against pseudotyped viruses with D614G spike proteins harboring single point mutations from the variants of concern (VOCs).
Omicron variant (B.1.1.529) of SARS-CoV-2: understanding mutations in the genome, S-glycoprotein, and antibody-binding regions.
Abstract: We also evaluated mutations in the antibody-binding regions and observed some important mutations overlapping those of previous variants including N501Y, D614G, H655Y, N679K, and P681H.
Result: Mutations previously reported as important in different variants were N501Y, D614G, H655Y, N679K, and P681H.
Discussion: The significant mutations and features are N501Y (augments the binding between of S-protein and ACE2); D614G (increase infectivity); H655Y (accelerate transmission);
COVID-19 outbreak in Malaysia: Decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang.
PMID: 35257681
2022
Journal of virological methods
Introduction: For the simultaneous detection of SARS-CoV-2 and its variant (D614G mutant), FAM and ROX fluorophores were attached to each probe during probe sequence design to enable target discrimination.
Introduction: In the experiment, the specificity and sensitivity of RdRp and N target primer pairs and probe sets were confirmed in the L clade, chosen to represent viruses containing the target gene without the D614G mutation.
Introduction: In this milieu, we developed a rRT-PCR detection method that simultaneously detects common SARS-CoV-2 and the SARS-CoV-2 D614 strain (a mutant with the replacement of aspartic acid with glycine at position 614 of the spike glycoprotein (S protein)), which has become a major circulating strain worldwid
Evolution of the SARS-CoV-2 spike protein in the human host.
Result: Our binding data on the variants and engineered constructs show that the D614G substitution is a prerequisite for the tighter receptor binding of changes in RBD, like N501Y, but do not explain how it facilitates the increase in affinity.
Result: The substitution D614G (relative to Wuhan) occurred earlier in the evolution of SARS-CoV-2, became the predominant global form of the virus and continues to be present in the Alpha and Beta variant forms of the virus.
Result: They follow D614G substitution which was acquired early in the pandemic and similarly acted to increase the spike stability.
Result: We suggest that one of effects of the more open conformation promoted by the D614G substitution is to increase the proportion o
Travel ban effects on SARS-CoV-2 transmission lineages in the UAE as inferred by genomic epidemiology.
Abstract: The study comprehensively characterized the genomic aspects of the virus and its spread within the UAE and identified that the prevalence shift of the D614G mutation was due to the later introductions of the G-variant associated with international travel, rather than higher local transmissibility.
Result: In addition to the above-mentioned D614G mutation, we observe four cases of a nearby E583D mutation, that could be of diagnostic or clinical relevance (transmissibility and severity).
Result: Prevalence shift of D614G variants.
Result: We also observed a shift of prevalence for strains with D614G mutations in the viral Spike (Figure: A shift of prevalence of strains with D614G mutations.
Rapidly Identifying New Coronavirus Mutations of Potential Concern in the Omicron Variant Using an Unsupervised Learning Strategy.
Abstract: To build an investigative framework, we have applied an unsupervised machine learning approach to 4296 Omicron viral genomes collected and deposited to GISAID as of December 14, 2021, and have identified a core haplotype of 28 polymutants (A67V, T95I, G339D, R346K, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H,
"VE607 Stabilizes SARS-CoV-2 Spike In the ""RBD-up"" Conformation and Inhibits Viral Entry."
Abstract: The IC 50 values are in the low micromolar range for pseudoparticles derived from SARS-CoV-2 Wuhan/D614G as well as from variants of concern (Alpha, Beta, Gamma, Delta and Omicron), suggesting that VE607 has potential for the development of drugs against SARS-CoV-2 infections.
Coronavirus Genomes and Unique Mutations in Structural and Non-Structural Proteins in Pakistani SARS-CoV-2 Delta Variants during the Fourth Wave of the Pandemic.
Result: The most common non-synonymous and indel mutations, present in all complete genome samples were spike_T478K, spike_T19R, spike_L452R, spike_F157del, spike_E156G, spike_P681R, spike_D614G, spike_R158del, and spike_G142D (Table 3).
SARS_CoV_2 mutation literature information.
PMID: 
2022
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